Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1728 and Rru_A1729 overlap by 4 nucleotides Rru_A1729 and Rru_A1730 are separated by 129 nucleotides Rru_A1730 and Rru_A1731 are separated by 20 nucleotides
Rru_A1728: Rru_A1728 - ATP-dependent DNA helicase RecG (NCBI), at 2,022,753 to 2,024,864
_A1728
Rru_A1729: Rru_A1729 - Protein of unknown function DUF502 (NCBI), at 2,024,861 to 2,025,637
_A1729
Rru_A1730: Rru_A1730 - nuclease (NCBI), at 2,025,767 to 2,026,294
_A1730
Rru_A1731: Rru_A1731 - hypothetical protein (NCBI), at 2,026,315 to 2,026,647
_A1731
Position (kb)
2024
2025
2026 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2024.082 kb on - strand, within Rru_A1728 at 2024.547 kb on + strand, within Rru_A1728 at 2025.198 kb on + strand, within Rru_A1729 at 2025.198 kb on + strand, within Rru_A1729 at 2025.429 kb on + strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.540 kb on + strand, within Rru_A1729 at 2025.549 kb on - strand, within Rru_A1729 at 2025.700 kb on + strand at 2025.705 kb on + strand at 2025.705 kb on + strand at 2025.705 kb on + strand at 2025.706 kb on - strand at 2025.710 kb on + strand at 2025.711 kb on - strand at 2025.917 kb on + strand, within Rru_A1730 at 2026.034 kb on + strand, within Rru_A1730 at 2026.035 kb on - strand, within Rru_A1730 at 2026.035 kb on - strand, within Rru_A1730 at 2026.035 kb on - strand, within Rru_A1730 at 2026.035 kb on - strand, within Rru_A1730 at 2026.070 kb on - strand, within Rru_A1730 at 2026.341 kb on + strand at 2026.342 kb on - strand at 2026.342 kb on - strand at 2026.343 kb on + strand at 2026.343 kb on + strand at 2026.343 kb on + strand at 2026.343 kb on + strand at 2026.343 kb on + strand at 2026.344 kb on - strand at 2026.344 kb on - strand at 2026.515 kb on - strand, within Rru_A1731 at 2026.515 kb on - strand, within Rru_A1731 at 2026.611 kb on - strand, within Rru_A1731 at 2026.611 kb on - strand, within Rru_A1731
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,024,082 - Rru_A1728 0.63 -0.8 2,024,547 + Rru_A1728 0.85 -1.5 2,025,198 + Rru_A1729 0.43 +1.3 2,025,198 + Rru_A1729 0.43 +0.8 2,025,429 + Rru_A1729 0.73 +0.8 2,025,430 - Rru_A1729 0.73 +0.5 2,025,430 - Rru_A1729 0.73 -1.3 2,025,430 - Rru_A1729 0.73 +0.7 2,025,430 - Rru_A1729 0.73 -0.7 2,025,540 + Rru_A1729 0.87 +1.6 2,025,549 - Rru_A1729 0.89 +1.4 2,025,700 + +0.2 2,025,705 + +0.5 2,025,705 + -1.5 2,025,705 + -1.0 2,025,706 - -0.4 2,025,710 + -1.2 2,025,711 - -2.2 2,025,917 + Rru_A1730 0.28 +2.6 2,026,034 + Rru_A1730 0.51 -3.2 2,026,035 - Rru_A1730 0.51 -0.1 2,026,035 - Rru_A1730 0.51 -0.2 2,026,035 - Rru_A1730 0.51 -1.5 2,026,035 - Rru_A1730 0.51 -1.8 2,026,070 - Rru_A1730 0.57 -0.2 2,026,341 + -1.0 2,026,342 - +0.5 2,026,342 - -1.5 2,026,343 + +2.1 2,026,343 + +1.2 2,026,343 + -0.5 2,026,343 + -1.1 2,026,343 + -3.0 2,026,344 - -0.8 2,026,344 - -1.9 2,026,515 - Rru_A1731 0.60 -1.8 2,026,515 - Rru_A1731 0.60 +0.6 2,026,611 - Rru_A1731 0.89 -0.2 2,026,611 - Rru_A1731 0.89 +1.6
Or see this region's nucleotide sequence