Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1726 and Rru_A1727 are separated by 4 nucleotides Rru_A1727 and Rru_A1728 are separated by 164 nucleotides Rru_A1728 and Rru_A1729 overlap by 4 nucleotides Rru_A1729 and Rru_A1730 are separated by 129 nucleotides
Rru_A1726: Rru_A1726 - Transcription-repair coupling factor (NCBI), at 2,018,784 to 2,022,317
_A1726
Rru_A1727: Rru_A1727 - Protein of unknown function DUF339 (NCBI), at 2,022,322 to 2,022,588
_A1727
Rru_A1728: Rru_A1728 - ATP-dependent DNA helicase RecG (NCBI), at 2,022,753 to 2,024,864
_A1728
Rru_A1729: Rru_A1729 - Protein of unknown function DUF502 (NCBI), at 2,024,861 to 2,025,637
_A1729
Rru_A1730: Rru_A1730 - nuclease (NCBI), at 2,025,767 to 2,026,294
_A1730
Position (kb)
2022
2023
2024
2025 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2021.796 kb on - strand, within Rru_A1726 at 2021.822 kb on - strand, within Rru_A1726 at 2021.857 kb on + strand, within Rru_A1726 at 2021.857 kb on + strand, within Rru_A1726 at 2021.858 kb on - strand, within Rru_A1726 at 2022.088 kb on + strand at 2022.089 kb on - strand at 2022.089 kb on - strand at 2022.654 kb on + strand at 2022.654 kb on + strand at 2022.654 kb on + strand at 2022.654 kb on + strand at 2022.654 kb on + strand at 2022.654 kb on + strand at 2022.655 kb on - strand at 2022.655 kb on - strand at 2022.655 kb on - strand at 2022.656 kb on + strand at 2022.656 kb on + strand at 2022.656 kb on + strand at 2022.656 kb on + strand at 2022.657 kb on - strand at 2022.657 kb on - strand at 2022.657 kb on - strand at 2022.657 kb on - strand at 2022.657 kb on - strand at 2022.658 kb on + strand at 2022.658 kb on + strand at 2022.658 kb on + strand at 2022.658 kb on + strand at 2022.659 kb on - strand at 2022.659 kb on - strand at 2024.082 kb on - strand, within Rru_A1728 at 2024.547 kb on + strand, within Rru_A1728 at 2025.198 kb on + strand, within Rru_A1729 at 2025.198 kb on + strand, within Rru_A1729 at 2025.429 kb on + strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.430 kb on - strand, within Rru_A1729 at 2025.540 kb on + strand, within Rru_A1729 at 2025.549 kb on - strand, within Rru_A1729 at 2025.700 kb on + strand at 2025.705 kb on + strand at 2025.705 kb on + strand at 2025.705 kb on + strand at 2025.706 kb on - strand at 2025.710 kb on + strand at 2025.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 2,021,796 - Rru_A1726 0.85 -2.4 2,021,822 - Rru_A1726 0.86 -2.5 2,021,857 + Rru_A1726 0.87 -2.5 2,021,857 + Rru_A1726 0.87 -3.6 2,021,858 - Rru_A1726 0.87 -0.2 2,022,088 + -2.6 2,022,089 - -0.8 2,022,089 - +1.8 2,022,654 + -4.4 2,022,654 + -1.4 2,022,654 + -1.9 2,022,654 + -3.5 2,022,654 + -0.4 2,022,654 + -0.2 2,022,655 - -1.9 2,022,655 - -1.7 2,022,655 - -1.4 2,022,656 + -3.0 2,022,656 + +1.8 2,022,656 + -2.9 2,022,656 + +0.2 2,022,657 - -0.8 2,022,657 - -1.2 2,022,657 - -1.7 2,022,657 - -1.2 2,022,657 - +1.8 2,022,658 + -3.2 2,022,658 + -0.5 2,022,658 + -1.2 2,022,658 + -2.4 2,022,659 - -1.2 2,022,659 - -1.1 2,024,082 - Rru_A1728 0.63 -0.8 2,024,547 + Rru_A1728 0.85 -1.5 2,025,198 + Rru_A1729 0.43 +1.3 2,025,198 + Rru_A1729 0.43 +0.8 2,025,429 + Rru_A1729 0.73 +0.8 2,025,430 - Rru_A1729 0.73 +0.5 2,025,430 - Rru_A1729 0.73 -1.3 2,025,430 - Rru_A1729 0.73 +0.7 2,025,430 - Rru_A1729 0.73 -0.7 2,025,540 + Rru_A1729 0.87 +1.6 2,025,549 - Rru_A1729 0.89 +1.4 2,025,700 + +0.2 2,025,705 + +0.5 2,025,705 + -1.5 2,025,705 + -1.0 2,025,706 - -0.4 2,025,710 + -1.2 2,025,711 - -2.2
Or see this region's nucleotide sequence