Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1357 and Rru_A1358 are separated by 165 nucleotides Rru_A1358 and Rru_A1359 are separated by 75 nucleotides Rru_A1359 and Rru_A1360 are separated by 23 nucleotides
Rru_A1357: Rru_A1357 - Transaldolase AB (NCBI), at 1,598,620 to 1,599,603
_A1357
Rru_A1358: Rru_A1358 - Triosephosphate isomerase (NCBI), at 1,599,769 to 1,600,545
_A1358
Rru_A1359: Rru_A1359 - Ribose/galactose isomerase (NCBI), at 1,600,621 to 1,601,097
_A1359
Rru_A1360: Rru_A1360 - Dak phosphatase (NCBI), at 1,601,121 to 1,601,768
_A1360
Position (kb)
1599
1600
1601 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1598.969 kb on + strand, within Rru_A1357 at 1598.969 kb on + strand, within Rru_A1357 at 1598.969 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.975 kb on + strand, within Rru_A1357 at 1598.976 kb on - strand, within Rru_A1357 at 1598.976 kb on - strand, within Rru_A1357 at 1599.080 kb on - strand, within Rru_A1357 at 1599.459 kb on - strand, within Rru_A1357 at 1599.459 kb on - strand, within Rru_A1357 at 1599.771 kb on - strand at 1600.049 kb on + strand, within Rru_A1358 at 1600.478 kb on + strand at 1600.479 kb on - strand at 1600.563 kb on + strand at 1600.653 kb on + strand at 1600.809 kb on + strand, within Rru_A1359 at 1600.811 kb on + strand, within Rru_A1359 at 1600.956 kb on - strand, within Rru_A1359 at 1601.000 kb on + strand, within Rru_A1359 at 1601.000 kb on + strand, within Rru_A1359 at 1601.001 kb on - strand, within Rru_A1359 at 1601.001 kb on - strand, within Rru_A1359 at 1601.077 kb on - strand at 1601.111 kb on + strand at 1601.111 kb on + strand at 1601.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 1,598,969 + Rru_A1357 0.35 -2.3 1,598,969 + Rru_A1357 0.35 -3.0 1,598,969 + Rru_A1357 0.35 -2.5 1,598,975 + Rru_A1357 0.36 -0.3 1,598,975 + Rru_A1357 0.36 +0.3 1,598,975 + Rru_A1357 0.36 -1.8 1,598,975 + Rru_A1357 0.36 -0.5 1,598,975 + Rru_A1357 0.36 +1.4 1,598,975 + Rru_A1357 0.36 -2.1 1,598,976 - Rru_A1357 0.36 -0.8 1,598,976 - Rru_A1357 0.36 +0.7 1,599,080 - Rru_A1357 0.47 -1.6 1,599,459 - Rru_A1357 0.85 +1.6 1,599,459 - Rru_A1357 0.85 -1.3 1,599,771 - -1.9 1,600,049 + Rru_A1358 0.36 -1.7 1,600,478 + -1.6 1,600,479 - -2.7 1,600,563 + -1.6 1,600,653 + +0.7 1,600,809 + Rru_A1359 0.39 -0.3 1,600,811 + Rru_A1359 0.40 -0.9 1,600,956 - Rru_A1359 0.70 -1.1 1,601,000 + Rru_A1359 0.79 -0.9 1,601,000 + Rru_A1359 0.79 -2.4 1,601,001 - Rru_A1359 0.80 +2.0 1,601,001 - Rru_A1359 0.80 -0.6 1,601,077 - -2.0 1,601,111 + -2.1 1,601,111 + -0.3 1,601,111 + -0.3
Or see this region's nucleotide sequence