Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1202 and Rru_A1203 are separated by 13 nucleotides Rru_A1203 and Rru_A1204 are separated by 5 nucleotides Rru_A1204 and Rru_A1205 are separated by 28 nucleotides Rru_A1205 and Rru_A1206 are separated by 123 nucleotides
Rru_A1202: Rru_A1202 - Succinate dehydrogenase, cytochrome b subunit (NCBI), at 1,419,991 to 1,420,389
_A1202
Rru_A1203: Rru_A1203 - Succinate dehydrogenase, cytochrome b subunit (NCBI), at 1,420,403 to 1,420,789
_A1203
Rru_A1204: Rru_A1204 - Succinate dehydrogenase flavoprotein subunit (NCBI), at 1,420,795 to 1,422,579
_A1204
Rru_A1205: Rru_A1205 - Succinate dehydrogenase/fumarate reductase iron-sulfur protein (NCBI), at 1,422,608 to 1,423,390
_A1205
Rru_A1206: Rru_A1206 - hypothetical protein (NCBI), at 1,423,514 to 1,424,092
_A1206
Position (kb)
1420
1421
1422
1423 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1419.801 kb on + strand at 1419.801 kb on + strand at 1419.802 kb on - strand at 1419.802 kb on - strand at 1419.922 kb on + strand at 1419.922 kb on + strand at 1423.481 kb on + strand at 1423.481 kb on + strand at 1423.482 kb on - strand at 1423.515 kb on + strand at 1423.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 1,419,801 + -1.3 1,419,801 + +0.6 1,419,802 - +0.3 1,419,802 - +0.8 1,419,922 + -2.8 1,419,922 + -3.1 1,423,481 + +0.8 1,423,481 + +0.8 1,423,482 - -1.7 1,423,515 + -3.6 1,423,515 + +0.6
Or see this region's nucleotide sequence