Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1062 and Rru_A1063 are separated by 37 nucleotides Rru_A1063 and Rru_A1064 are separated by 174 nucleotides Rru_A1064 and Rru_A1065 are separated by 11 nucleotides Rru_A1065 and Rru_A1066 are separated by 154 nucleotides Rru_A1066 and Rru_A1067 overlap by 4 nucleotides
Rru_A1062: Rru_A1062 - PilT protein-like (NCBI), at 1,254,841 to 1,255,206
_A1062
Rru_A1063: Rru_A1063 - putative transcription regulator protein (NCBI), at 1,255,244 to 1,255,588
_A1063
Rru_A1064: Rru_A1064 - Plasmid maintenance system killer (NCBI), at 1,255,763 to 1,256,059
_A1064
Rru_A1065: Rru_A1065 - Plasmid maintenance system antidote protein (NCBI), at 1,256,071 to 1,256,376
_A1065
Rru_A1066: Rru_A1066 - ABC transporter component (NCBI), at 1,256,531 to 1,257,316
_A1066
Rru_A1067: Rru_A1067 - Cobalamin synthesis protein/P47K (NCBI), at 1,257,313 to 1,258,014
_A1067
Position (kb) 1256
1257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1255.086 kb on + strand, within Rru_A1062 at 1255.086 kb on + strand, within Rru_A1062 at 1255.087 kb on - strand, within Rru_A1062 at 1255.087 kb on - strand, within Rru_A1062 at 1255.195 kb on + strand at 1255.195 kb on + strand at 1255.196 kb on - strand at 1255.205 kb on - strand at 1255.348 kb on + strand, within Rru_A1063 at 1255.572 kb on + strand at 1255.600 kb on + strand at 1255.600 kb on + strand at 1255.757 kb on + strand at 1255.936 kb on - strand, within Rru_A1064 at 1256.034 kb on - strand at 1256.747 kb on + strand, within Rru_A1066 at 1257.014 kb on - strand, within Rru_A1066 at 1257.014 kb on - strand, within Rru_A1066
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 1,255,086 + Rru_A1062 0.67 -0.3 1,255,086 + Rru_A1062 0.67 +1.7 1,255,087 - Rru_A1062 0.67 -1.6 1,255,087 - Rru_A1062 0.67 -0.7 1,255,195 + -1.7 1,255,195 + +1.3 1,255,196 - +0.9 1,255,205 - -1.2 1,255,348 + Rru_A1063 0.30 +0.3 1,255,572 + -1.9 1,255,600 + -0.2 1,255,600 + -1.0 1,255,757 + +1.0 1,255,936 - Rru_A1064 0.58 -0.6 1,256,034 - +3.5 1,256,747 + Rru_A1066 0.27 +0.8 1,257,014 - Rru_A1066 0.61 -3.2 1,257,014 - Rru_A1066 0.61 -0.9
Or see this region's nucleotide sequence