Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0618 and Rru_A0619 overlap by 13 nucleotides Rru_A0619 and Rru_A0620 overlap by 1 nucleotides Rru_A0620 and Rru_A0621 are separated by 34 nucleotides
Rru_A0618: Rru_A0618 - PUCC protein (NCBI), at 732,044 to 733,486
_A0618
Rru_A0619: Rru_A0619 - Magnesium protoporphyrin O-methyltransferase (NCBI), at 733,474 to 734,184
_A0619
Rru_A0620: Rru_A0620 - Light-independent protochlorophyllide reductase, iron-sulfur ATP-binding protein (NCBI), at 734,184 to 735,071
_A0620
Rru_A0621: Rru_A0621 - Magnesium-chelatase, subunit H (NCBI), at 735,106 to 738,834
_A0621
Position (kb) 733
734
735 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 732.630 kb on + strand, within Rru_A0618 at 732.630 kb on + strand, within Rru_A0618 at 732.631 kb on - strand, within Rru_A0618 at 732.631 kb on - strand, within Rru_A0618 at 732.936 kb on + strand, within Rru_A0618 at 732.936 kb on + strand, within Rru_A0618 at 732.936 kb on + strand, within Rru_A0618 at 732.937 kb on - strand, within Rru_A0618 at 732.937 kb on - strand, within Rru_A0618 at 732.937 kb on - strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.168 kb on + strand, within Rru_A0618 at 733.169 kb on - strand, within Rru_A0618 at 733.169 kb on - strand, within Rru_A0618 at 733.169 kb on - strand, within Rru_A0618 at 733.169 kb on - strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.170 kb on + strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.171 kb on - strand, within Rru_A0618 at 733.518 kb on - strand at 733.518 kb on - strand at 733.518 kb on - strand at 733.518 kb on - strand at 733.770 kb on - strand, within Rru_A0619 at 733.770 kb on - strand, within Rru_A0619 at 733.914 kb on + strand, within Rru_A0619 at 734.139 kb on - strand at 734.168 kb on + strand at 734.168 kb on + strand at 734.168 kb on + strand at 734.168 kb on + strand at 734.169 kb on - strand at 734.169 kb on - strand at 734.220 kb on + strand at 734.221 kb on - strand at 734.221 kb on - strand at 734.281 kb on + strand, within Rru_A0620 at 734.282 kb on - strand, within Rru_A0620 at 734.476 kb on + strand, within Rru_A0620 at 734.476 kb on + strand, within Rru_A0620 at 734.476 kb on + strand, within Rru_A0620 at 734.476 kb on + strand, within Rru_A0620 at 734.692 kb on + strand, within Rru_A0620 at 734.692 kb on + strand, within Rru_A0620 at 734.752 kb on + strand, within Rru_A0620 at 734.753 kb on - strand, within Rru_A0620 at 734.753 kb on - strand, within Rru_A0620 at 734.753 kb on - strand, within Rru_A0620 at 734.753 kb on - strand, within Rru_A0620 at 734.968 kb on + strand, within Rru_A0620 at 734.968 kb on + strand, within Rru_A0620 at 734.968 kb on + strand, within Rru_A0620 at 734.968 kb on + strand, within Rru_A0620 at 734.969 kb on - strand, within Rru_A0620 at 735.074 kb on + strand at 735.075 kb on - strand at 735.075 kb on - strand at 735.075 kb on - strand at 735.078 kb on + strand at 735.078 kb on + strand at 735.078 kb on + strand at 735.079 kb on - strand at 735.127 kb on + strand at 735.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 732,630 + Rru_A0618 0.41 +0.9 732,630 + Rru_A0618 0.41 -2.3 732,631 - Rru_A0618 0.41 +0.3 732,631 - Rru_A0618 0.41 -1.5 732,936 + Rru_A0618 0.62 -0.9 732,936 + Rru_A0618 0.62 -0.7 732,936 + Rru_A0618 0.62 -2.2 732,937 - Rru_A0618 0.62 -4.2 732,937 - Rru_A0618 0.62 -3.0 732,937 - Rru_A0618 0.62 -1.5 733,168 + Rru_A0618 0.78 -3.3 733,168 + Rru_A0618 0.78 -2.0 733,168 + Rru_A0618 0.78 -3.5 733,168 + Rru_A0618 0.78 -1.3 733,168 + Rru_A0618 0.78 -2.3 733,168 + Rru_A0618 0.78 -4.3 733,168 + Rru_A0618 0.78 +1.9 733,169 - Rru_A0618 0.78 -1.7 733,169 - Rru_A0618 0.78 -3.9 733,169 - Rru_A0618 0.78 -2.6 733,169 - Rru_A0618 0.78 -3.1 733,170 + Rru_A0618 0.78 -2.9 733,170 + Rru_A0618 0.78 -2.9 733,170 + Rru_A0618 0.78 -2.1 733,170 + Rru_A0618 0.78 -0.1 733,170 + Rru_A0618 0.78 -0.8 733,170 + Rru_A0618 0.78 -3.9 733,170 + Rru_A0618 0.78 +0.3 733,170 + Rru_A0618 0.78 -3.3 733,170 + Rru_A0618 0.78 -3.1 733,170 + Rru_A0618 0.78 -0.8 733,170 + Rru_A0618 0.78 -2.8 733,171 - Rru_A0618 0.78 -0.5 733,171 - Rru_A0618 0.78 -2.1 733,171 - Rru_A0618 0.78 +1.9 733,171 - Rru_A0618 0.78 -0.1 733,171 - Rru_A0618 0.78 -0.5 733,171 - Rru_A0618 0.78 -0.5 733,171 - Rru_A0618 0.78 -3.3 733,171 - Rru_A0618 0.78 -1.8 733,171 - Rru_A0618 0.78 -1.5 733,518 - +1.9 733,518 - -2.2 733,518 - -0.7 733,518 - -1.7 733,770 - Rru_A0619 0.42 -1.3 733,770 - Rru_A0619 0.42 -1.8 733,914 + Rru_A0619 0.62 -1.9 734,139 - -1.6 734,168 + -1.6 734,168 + +1.9 734,168 + -1.5 734,168 + -3.8 734,169 - -2.3 734,169 - -0.8 734,220 + -1.9 734,221 - -1.5 734,221 - +0.3 734,281 + Rru_A0620 0.11 -1.8 734,282 - Rru_A0620 0.11 -5.1 734,476 + Rru_A0620 0.33 -4.0 734,476 + Rru_A0620 0.33 +0.5 734,476 + Rru_A0620 0.33 -0.7 734,476 + Rru_A0620 0.33 +0.3 734,692 + Rru_A0620 0.57 -2.7 734,692 + Rru_A0620 0.57 -0.7 734,752 + Rru_A0620 0.64 -1.7 734,753 - Rru_A0620 0.64 -2.4 734,753 - Rru_A0620 0.64 -2.5 734,753 - Rru_A0620 0.64 +2.4 734,753 - Rru_A0620 0.64 -2.9 734,968 + Rru_A0620 0.88 -1.5 734,968 + Rru_A0620 0.88 -4.4 734,968 + Rru_A0620 0.88 +1.9 734,968 + Rru_A0620 0.88 -3.0 734,969 - Rru_A0620 0.88 -0.1 735,074 + -0.6 735,075 - -0.1 735,075 - -1.6 735,075 - +1.9 735,078 + -1.6 735,078 + -1.3 735,078 + -1.6 735,079 - -1.1 735,127 + -2.4 735,127 + +1.9
Or see this region's nucleotide sequence