Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0584 and Rru_A0585 overlap by 4 nucleotides Rru_A0585 and Rru_A0586 are separated by 120 nucleotides Rru_A0586 and Rru_A0587 are separated by 79 nucleotides
Rru_A0584: Rru_A0584 - inner-membrane translocator (NCBI), at 693,637 to 695,478
_A0584
Rru_A0585: Rru_A0585 - ABC transporter component (NCBI), at 695,475 to 696,254
_A0585
Rru_A0586: Rru_A0586 - Chaperonin Cpn10 (NCBI), at 696,375 to 696,662
_A0586
Rru_A0587: Rru_A0587 - Chaperonin Cpn60/TCP-1 (NCBI), at 696,742 to 698,388
_A0587
Position (kb) 695
696
697 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 694.627 kb on + strand, within Rru_A0584 at 694.627 kb on + strand, within Rru_A0584 at 694.627 kb on + strand, within Rru_A0584 at 694.627 kb on + strand, within Rru_A0584 at 694.628 kb on - strand, within Rru_A0584 at 694.628 kb on - strand, within Rru_A0584 at 694.877 kb on - strand, within Rru_A0584 at 694.877 kb on - strand, within Rru_A0584 at 695.332 kb on + strand at 695.333 kb on - strand at 695.333 kb on - strand at 695.333 kb on - strand at 695.914 kb on - strand, within Rru_A0585 at 696.198 kb on + strand at 696.199 kb on - strand at 696.199 kb on - strand at 696.222 kb on - strand at 696.278 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 694,627 + Rru_A0584 0.54 +0.4 694,627 + Rru_A0584 0.54 -2.1 694,627 + Rru_A0584 0.54 +1.3 694,627 + Rru_A0584 0.54 -0.4 694,628 - Rru_A0584 0.54 +0.9 694,628 - Rru_A0584 0.54 +0.9 694,877 - Rru_A0584 0.67 +1.2 694,877 - Rru_A0584 0.67 +1.0 695,332 + -2.0 695,333 - -2.9 695,333 - +0.3 695,333 - +4.5 695,914 - Rru_A0585 0.56 -1.2 696,198 + +0.2 696,199 - -1.6 696,199 - -1.9 696,222 - -0.1 696,278 + -1.7
Or see this region's nucleotide sequence