Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0406 and Rru_A0407 are separated by 49 nucleotides Rru_A0407 and Rru_A0408 overlap by 4 nucleotides Rru_A0408 and Rru_A0409 overlap by 4 nucleotides
Rru_A0406: Rru_A0406 - DNA repair protein RadA (NCBI), at 482,981 to 484,390
_A0406
Rru_A0407: Rru_A0407 - ABC transporter component (NCBI), at 484,440 to 485,213
_A0407
Rru_A0408: Rru_A0408 - Protein of unknown function DUF140 (NCBI), at 485,210 to 485,983
_A0408
Rru_A0409: Rru_A0409 - Alanine racemase region (NCBI), at 485,980 to 487,104
_A0409
Position (kb) 484
485
486 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 483.682 kb on - strand, within Rru_A0406 at 483.700 kb on + strand, within Rru_A0406 at 483.700 kb on + strand, within Rru_A0406 at 483.700 kb on + strand, within Rru_A0406 at 483.701 kb on - strand, within Rru_A0406 at 483.701 kb on - strand, within Rru_A0406 at 483.701 kb on - strand, within Rru_A0406 at 483.858 kb on + strand, within Rru_A0406 at 483.858 kb on + strand, within Rru_A0406 at 483.859 kb on - strand, within Rru_A0406 at 484.027 kb on + strand, within Rru_A0406 at 484.027 kb on + strand, within Rru_A0406 at 484.027 kb on + strand, within Rru_A0406 at 484.027 kb on + strand, within Rru_A0406 at 484.027 kb on + strand, within Rru_A0406 at 484.028 kb on - strand, within Rru_A0406 at 484.028 kb on - strand, within Rru_A0406 at 484.029 kb on + strand, within Rru_A0406 at 484.029 kb on + strand, within Rru_A0406 at 484.029 kb on + strand, within Rru_A0406 at 484.030 kb on - strand, within Rru_A0406 at 484.341 kb on + strand at 484.341 kb on + strand at 484.485 kb on + strand at 486.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 483,682 - Rru_A0406 0.50 +0.4 483,700 + Rru_A0406 0.51 +2.4 483,700 + Rru_A0406 0.51 +2.3 483,700 + Rru_A0406 0.51 +2.3 483,701 - Rru_A0406 0.51 +0.4 483,701 - Rru_A0406 0.51 -0.1 483,701 - Rru_A0406 0.51 +2.0 483,858 + Rru_A0406 0.62 +1.7 483,858 + Rru_A0406 0.62 +2.9 483,859 - Rru_A0406 0.62 -1.9 484,027 + Rru_A0406 0.74 +3.0 484,027 + Rru_A0406 0.74 +2.4 484,027 + Rru_A0406 0.74 +0.3 484,027 + Rru_A0406 0.74 -1.1 484,027 + Rru_A0406 0.74 -1.1 484,028 - Rru_A0406 0.74 +0.6 484,028 - Rru_A0406 0.74 +2.3 484,029 + Rru_A0406 0.74 -2.0 484,029 + Rru_A0406 0.74 +1.9 484,029 + Rru_A0406 0.74 +1.8 484,030 - Rru_A0406 0.74 +3.8 484,341 + +1.8 484,341 + +2.4 484,485 + -3.0 486,064 - +0.3
Or see this region's nucleotide sequence