Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0294 and Rru_A0295 are separated by 82 nucleotides Rru_A0295 and Rru_A0296 are separated by 298 nucleotides Rru_A0296 and Rru_AR0012 are separated by 224 nucleotides
Rru_A0294: Rru_A0294 - Cytidylate kinase (NCBI), at 360,174 to 360,815
_A0294
Rru_A0295: Rru_A0295 - 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI), at 360,898 to 362,253
_A0295
Rru_A0296: Rru_A0296 - hypothetical protein (NCBI), at 362,552 to 362,881
_A0296
Rru_AR0012: Rru_AR0012 - tRNA-Ala (NCBI), at 363,106 to 363,181
_AR0012
Position (kb) 360
361
362
363 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 360.125 kb on - strand at 360.125 kb on - strand at 360.125 kb on - strand at 360.719 kb on + strand, within Rru_A0294 at 360.810 kb on - strand at 362.405 kb on + strand at 362.405 kb on + strand at 362.406 kb on - strand at 362.406 kb on - strand at 362.423 kb on + strand at 362.424 kb on - strand at 362.456 kb on + strand at 362.456 kb on + strand at 362.457 kb on - strand at 362.544 kb on + strand at 362.544 kb on + strand at 362.545 kb on - strand at 362.609 kb on + strand, within Rru_A0296 at 362.610 kb on - strand, within Rru_A0296 at 362.788 kb on + strand, within Rru_A0296 at 362.788 kb on + strand, within Rru_A0296 at 362.880 kb on - strand at 362.880 kb on - strand at 362.880 kb on - strand at 362.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 360,125 - +2.1 360,125 - +0.1 360,125 - -1.6 360,719 + Rru_A0294 0.85 -0.9 360,810 - -0.1 362,405 + -2.3 362,405 + -2.2 362,406 - -1.3 362,406 - -2.0 362,423 + +2.8 362,424 - -0.4 362,456 + -0.9 362,456 + -0.3 362,457 - -2.7 362,544 + -2.4 362,544 + -1.1 362,545 - +2.1 362,609 + Rru_A0296 0.17 -0.3 362,610 - Rru_A0296 0.18 -1.3 362,788 + Rru_A0296 0.72 +0.5 362,788 + Rru_A0296 0.72 -1.3 362,880 - +1.4 362,880 - +1.1 362,880 - -1.9 362,974 - +0.1
Or see this region's nucleotide sequence