Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0270 and Rru_A0271 are separated by 172 nucleotides Rru_A0271 and Rru_A0272 are separated by 92 nucleotides Rru_A0272 and Rru_A0273 are separated by 69 nucleotides Rru_A0273 and Rru_A0274 are separated by 183 nucleotides
Rru_A0270: Rru_A0270 - Protein of unknown function DUF525 (NCBI), at 335,633 to 336,040
_A0270
Rru_A0271: Rru_A0271 - GTP cyclohydrolase I (NCBI), at 336,213 to 336,917
_A0271
Rru_A0272: Rru_A0272 - Small multidrug resistance protein (NCBI), at 337,010 to 337,321
_A0272
Rru_A0273: Rru_A0273 - Acetoacetyl-CoA reductase (NCBI), at 337,391 to 338,119
_A0273
Rru_A0274: Rru_A0274 - Acetyl-CoA C-acetyltransferase (NCBI), at 338,303 to 339,475
_A0274
Position (kb) 337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 336.115 kb on + strand at 336.115 kb on + strand at 336.115 kb on + strand at 337.041 kb on + strand at 337.041 kb on + strand at 337.392 kb on + strand at 337.392 kb on + strand at 337.393 kb on - strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.395 kb on + strand at 337.396 kb on - strand at 337.396 kb on - strand at 337.396 kb on - strand at 337.396 kb on - strand at 337.396 kb on - strand at 337.396 kb on - strand at 337.440 kb on + strand at 337.440 kb on + strand at 337.440 kb on + strand at 337.440 kb on + strand at 337.441 kb on - strand at 337.441 kb on - strand at 337.545 kb on + strand, within Rru_A0273 at 337.579 kb on - strand, within Rru_A0273 at 337.674 kb on + strand, within Rru_A0273 at 337.689 kb on + strand, within Rru_A0273 at 337.690 kb on - strand, within Rru_A0273 at 337.807 kb on + strand, within Rru_A0273 at 337.807 kb on + strand, within Rru_A0273 at 337.938 kb on + strand, within Rru_A0273 at 337.939 kb on - strand, within Rru_A0273 at 337.939 kb on - strand, within Rru_A0273 at 338.013 kb on + strand, within Rru_A0273 at 338.016 kb on + strand, within Rru_A0273 at 338.016 kb on + strand, within Rru_A0273 at 338.017 kb on - strand, within Rru_A0273 at 338.017 kb on - strand, within Rru_A0273 at 338.017 kb on - strand, within Rru_A0273 at 338.145 kb on + strand at 338.214 kb on + strand at 338.214 kb on + strand at 338.214 kb on + strand at 338.214 kb on + strand at 338.214 kb on + strand at 338.215 kb on - strand at 338.215 kb on - strand at 338.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 336,115 + +1.1 336,115 + -2.5 336,115 + +1.2 337,041 + +0.2 337,041 + +0.1 337,392 + -0.3 337,392 + -0.9 337,393 - -2.3 337,395 + +0.4 337,395 + +1.6 337,395 + +2.1 337,395 + -0.3 337,395 + -0.8 337,395 + -0.4 337,395 + -0.3 337,396 - -3.2 337,396 - -2.5 337,396 - -2.9 337,396 - -1.8 337,396 - -1.6 337,396 - -1.2 337,440 + -0.3 337,440 + -1.1 337,440 + -1.9 337,440 + -2.4 337,441 - -0.8 337,441 - -0.8 337,545 + Rru_A0273 0.21 -2.2 337,579 - Rru_A0273 0.26 -2.3 337,674 + Rru_A0273 0.39 +1.2 337,689 + Rru_A0273 0.41 -2.3 337,690 - Rru_A0273 0.41 -0.3 337,807 + Rru_A0273 0.57 -2.6 337,807 + Rru_A0273 0.57 -1.4 337,938 + Rru_A0273 0.75 -0.3 337,939 - Rru_A0273 0.75 -3.0 337,939 - Rru_A0273 0.75 +0.1 338,013 + Rru_A0273 0.85 +0.1 338,016 + Rru_A0273 0.86 -3.4 338,016 + Rru_A0273 0.86 -3.5 338,017 - Rru_A0273 0.86 +2.1 338,017 - Rru_A0273 0.86 -1.1 338,017 - Rru_A0273 0.86 -2.3 338,145 + +1.6 338,214 + +2.1 338,214 + -0.6 338,214 + -0.5 338,214 + -1.6 338,214 + -1.0 338,215 - +2.9 338,215 - +0.2 338,305 - +2.1
Or see this region's nucleotide sequence