Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0219 and Rru_A0220 are separated by 65 nucleotides Rru_A0220 and Rru_A0221 are separated by 197 nucleotides Rru_A0221 and Rru_A0222 are separated by 94 nucleotides
Rru_A0219: Rru_A0219 - hypothetical protein (NCBI), at 271,269 to 271,517
_A0219
Rru_A0220: Rru_A0220 - hypothetical protein (NCBI), at 271,583 to 272,059
_A0220
Rru_A0221: Rru_A0221 - Phosphoglycerate kinase (NCBI), at 272,257 to 273,450
_A0221
Rru_A0222: Rru_A0222 - Glyceraldehyde-3-phosphate dehydrogenase, type I (NCBI), at 273,545 to 274,552
_A0222
Position (kb) 272
273
274 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 271.295 kb on + strand, within Rru_A0219 at 271.295 kb on + strand, within Rru_A0219 at 271.296 kb on - strand, within Rru_A0219 at 271.296 kb on - strand, within Rru_A0219 at 271.301 kb on + strand, within Rru_A0219 at 271.302 kb on - strand, within Rru_A0219 at 271.383 kb on + strand, within Rru_A0219 at 271.383 kb on + strand, within Rru_A0219 at 271.434 kb on + strand, within Rru_A0219 at 271.434 kb on + strand, within Rru_A0219 at 271.434 kb on + strand, within Rru_A0219 at 271.435 kb on - strand, within Rru_A0219 at 271.435 kb on - strand, within Rru_A0219 at 271.435 kb on - strand, within Rru_A0219 at 271.438 kb on - strand, within Rru_A0219 at 271.438 kb on - strand, within Rru_A0219 at 271.515 kb on + strand at 271.515 kb on + strand at 271.516 kb on - strand at 271.516 kb on - strand at 271.516 kb on - strand at 271.516 kb on - strand at 271.516 kb on - strand at 271.831 kb on - strand, within Rru_A0220 at 271.983 kb on + strand, within Rru_A0220 at 271.983 kb on + strand, within Rru_A0220 at 272.152 kb on - strand at 272.166 kb on - strand at 272.258 kb on + strand at 272.259 kb on - strand at 273.522 kb on + strand at 273.522 kb on + strand at 273.546 kb on + strand at 273.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 271,295 + Rru_A0219 0.10 +1.9 271,295 + Rru_A0219 0.10 +0.2 271,296 - Rru_A0219 0.11 +0.5 271,296 - Rru_A0219 0.11 -0.7 271,301 + Rru_A0219 0.13 +0.4 271,302 - Rru_A0219 0.13 -1.0 271,383 + Rru_A0219 0.46 -0.0 271,383 + Rru_A0219 0.46 -1.2 271,434 + Rru_A0219 0.66 -1.3 271,434 + Rru_A0219 0.66 -2.1 271,434 + Rru_A0219 0.66 +0.7 271,435 - Rru_A0219 0.67 +1.0 271,435 - Rru_A0219 0.67 -1.6 271,435 - Rru_A0219 0.67 -1.3 271,438 - Rru_A0219 0.68 -2.2 271,438 - Rru_A0219 0.68 -0.1 271,515 + +2.2 271,515 + -1.8 271,516 - +0.1 271,516 - +0.4 271,516 - -2.1 271,516 - -2.4 271,516 - -0.1 271,831 - Rru_A0220 0.52 -0.8 271,983 + Rru_A0220 0.84 -2.7 271,983 + Rru_A0220 0.84 +0.9 272,152 - -0.3 272,166 - -0.5 272,258 + +0.0 272,259 - +0.8 273,522 + -4.0 273,522 + -2.2 273,546 + -2.1 273,547 - -0.3
Or see this region's nucleotide sequence