Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0121 and Rru_A0122 are separated by 17 nucleotides Rru_A0122 and Rru_A0123 overlap by 1 nucleotides Rru_A0123 and Rru_A0124 are separated by 296 nucleotides
Rru_A0121: Rru_A0121 - hypothetical protein (NCBI), at 141,370 to 142,092
_A0121
Rru_A0122: Rru_A0122 - hypothetical protein (NCBI), at 142,110 to 142,472
_A0122
Rru_A0123: Rru_A0123 - RfaE-HldE bifunctional protein, D-beta-D-heptose 7-phosphate kinase, D-beta-D-heptose 1-phosphate adenosyltransferase (NCBI), at 142,472 to 143,962
_A0123
Rru_A0124: Rru_A0124 - Putative diguanylate phosphodiesterase (EAL domain) (NCBI), at 144,259 to 145,608
_A0124
Position (kb) 142
143
144 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 141.507 kb on + strand, within Rru_A0121 at 141.508 kb on - strand, within Rru_A0121 at 141.508 kb on - strand, within Rru_A0121 at 141.741 kb on - strand, within Rru_A0121 at 141.741 kb on - strand, within Rru_A0121 at 141.745 kb on + strand, within Rru_A0121 at 141.746 kb on - strand, within Rru_A0121 at 141.771 kb on + strand, within Rru_A0121 at 141.771 kb on + strand, within Rru_A0121 at 141.771 kb on + strand, within Rru_A0121 at 142.145 kb on + strand at 142.145 kb on + strand at 142.145 kb on + strand at 142.145 kb on + strand at 142.145 kb on + strand at 142.146 kb on - strand at 142.146 kb on - strand at 144.002 kb on + strand at 144.002 kb on + strand at 144.002 kb on + strand at 144.002 kb on + strand at 144.003 kb on - strand at 144.003 kb on - strand at 144.003 kb on - strand at 144.025 kb on - strand at 144.025 kb on - strand at 144.137 kb on - strand at 144.184 kb on - strand at 144.206 kb on - strand at 144.460 kb on + strand, within Rru_A0124 at 144.460 kb on + strand, within Rru_A0124 at 144.461 kb on - strand, within Rru_A0124 at 144.765 kb on + strand, within Rru_A0124
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 141,507 + Rru_A0121 0.19 -1.6 141,508 - Rru_A0121 0.19 +1.6 141,508 - Rru_A0121 0.19 -0.7 141,741 - Rru_A0121 0.51 -3.5 141,741 - Rru_A0121 0.51 +0.8 141,745 + Rru_A0121 0.52 +1.3 141,746 - Rru_A0121 0.52 -2.4 141,771 + Rru_A0121 0.55 +0.8 141,771 + Rru_A0121 0.55 -1.9 141,771 + Rru_A0121 0.55 -4.3 142,145 + +1.0 142,145 + +1.9 142,145 + +0.0 142,145 + +2.1 142,145 + +0.9 142,146 - +0.0 142,146 - -1.5 144,002 + +0.5 144,002 + -0.3 144,002 + +2.0 144,002 + +0.8 144,003 - -0.6 144,003 - +2.0 144,003 - -1.0 144,025 - -0.3 144,025 - -1.2 144,137 - +0.3 144,184 - +3.4 144,206 - -0.6 144,460 + Rru_A0124 0.15 +0.0 144,460 + Rru_A0124 0.15 +0.0 144,461 - Rru_A0124 0.15 -1.4 144,765 + Rru_A0124 0.37 -1.4
Or see this region's nucleotide sequence