Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0070 and Rru_A0071 are separated by 327 nucleotides Rru_A0071 and Rru_A0072 are separated by 101 nucleotides Rru_A0072 and Rru_A0073 are separated by 412 nucleotides Rru_A0073 and Rru_A0074 are separated by 116 nucleotides
Rru_A0070: Rru_A0070 - Protein of unknown function DUF6, transmembrane (NCBI), at 81,316 to 82,311
_A0070
Rru_A0071: Rru_A0071 - hypothetical protein (NCBI), at 82,639 to 82,902
_A0071
Rru_A0072: Rru_A0072 - chaperone protein Hsp90 (NCBI), at 83,004 to 84,884
_A0072
Rru_A0073: Rru_A0073 - ribosomal subunit interface protein, putative (NCBI), at 85,297 to 85,677
_A0073
Rru_A0074: Rru_A0074 - Helicase-like (NCBI), at 85,794 to 88,994
_A0074
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 82.135 kb on - strand, within Rru_A0070 at 82.165 kb on + strand, within Rru_A0070 at 82.165 kb on + strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.326 kb on - strand at 82.329 kb on - strand at 82.329 kb on - strand at 82.386 kb on + strand at 82.386 kb on + strand at 82.387 kb on - strand at 82.387 kb on - strand at 82.396 kb on - strand at 82.396 kb on - strand at 82.621 kb on - strand at 82.621 kb on - strand at 82.659 kb on + strand at 82.659 kb on + strand at 82.660 kb on - strand at 82.660 kb on - strand at 82.734 kb on + strand, within Rru_A0071 at 82.734 kb on + strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.753 kb on - strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 83.005 kb on + strand at 83.005 kb on + strand at 83.006 kb on - strand at 83.006 kb on - strand at 85.072 kb on + strand at 85.079 kb on - strand at 85.151 kb on + strand at 85.271 kb on + strand at 85.271 kb on + strand at 85.271 kb on + strand at 85.272 kb on - strand at 85.272 kb on - strand at 85.403 kb on + strand, within Rru_A0073 at 85.404 kb on - strand, within Rru_A0073 at 85.438 kb on - strand, within Rru_A0073 at 85.458 kb on + strand, within Rru_A0073 at 85.458 kb on + strand, within Rru_A0073 at 85.459 kb on - strand, within Rru_A0073 at 85.621 kb on - strand, within Rru_A0073 at 85.675 kb on + strand at 85.675 kb on + strand at 85.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 82,135 - Rru_A0070 0.82 -0.4 82,165 + Rru_A0070 0.85 -1.6 82,165 + Rru_A0070 0.85 +0.3 82,166 - Rru_A0070 0.85 +0.4 82,166 - Rru_A0070 0.85 +3.0 82,166 - Rru_A0070 0.85 -1.3 82,166 - Rru_A0070 0.85 +1.2 82,326 - -0.3 82,329 - -2.7 82,329 - -3.8 82,386 + +0.6 82,386 + +0.0 82,387 - -1.6 82,387 - +0.0 82,396 - -2.5 82,396 - -4.0 82,621 - -1.0 82,621 - -2.7 82,659 + -2.7 82,659 + -1.6 82,660 - +0.7 82,660 - -1.6 82,734 + Rru_A0071 0.36 +1.3 82,734 + Rru_A0071 0.36 +0.0 82,735 - Rru_A0071 0.36 -2.1 82,735 - Rru_A0071 0.36 -4.5 82,735 - Rru_A0071 0.36 -2.6 82,735 - Rru_A0071 0.36 -1.9 82,753 - Rru_A0071 0.43 -2.7 82,765 + Rru_A0071 0.48 -2.3 82,765 + Rru_A0071 0.48 -0.1 82,765 + Rru_A0071 0.48 -3.2 83,005 + +5.8 83,005 + -2.5 83,006 - -0.2 83,006 - -2.3 85,072 + -4.0 85,079 - -1.4 85,151 + -0.1 85,271 + -0.2 85,271 + +3.4 85,271 + -0.3 85,272 - -1.0 85,272 - -0.1 85,403 + Rru_A0073 0.28 +0.4 85,404 - Rru_A0073 0.28 -0.8 85,438 - Rru_A0073 0.37 -1.3 85,458 + Rru_A0073 0.42 -0.1 85,458 + Rru_A0073 0.42 -1.0 85,459 - Rru_A0073 0.43 -0.6 85,621 - Rru_A0073 0.85 -0.2 85,675 + -3.2 85,675 + -2.2 85,759 + -0.3
Or see this region's nucleotide sequence