Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0059 and Rru_A0060 are separated by 268 nucleotides Rru_A0060 and Rru_A0061 overlap by 15 nucleotides Rru_A0061 and Rru_A0062 are separated by 41 nucleotides
Rru_A0059: Rru_A0059 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (NCBI), at 66,362 to 67,372
_A0059
Rru_A0060: Rru_A0060 - Radical SAM (NCBI), at 67,641 to 68,804
_A0060
Rru_A0061: Rru_A0061 - Purine and other phosphorylases, family 1 (NCBI), at 68,790 to 69,518
_A0061
Rru_A0062: Rru_A0062 - terpene synthase, squalene cyclase (NCBI), at 69,560 to 71,530
_A0062
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 67.347 kb on + strand at 67.370 kb on + strand at 67.583 kb on + strand at 67.583 kb on + strand at 67.626 kb on + strand at 67.626 kb on + strand at 67.626 kb on + strand at 67.626 kb on + strand at 67.626 kb on + strand at 67.628 kb on + strand at 67.628 kb on + strand at 67.628 kb on + strand at 67.628 kb on + strand at 67.628 kb on + strand at 67.628 kb on + strand at 68.802 kb on + strand at 69.068 kb on + strand, within Rru_A0061
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 67,347 + -1.7 67,370 + +0.0 67,583 + -3.5 67,583 + +0.0 67,626 + +0.9 67,626 + +0.0 67,626 + +0.6 67,626 + +0.9 67,626 + +0.3 67,628 + +1.9 67,628 + -1.5 67,628 + +2.0 67,628 + +2.2 67,628 + -1.2 67,628 + +2.2 68,802 + +2.0 69,068 + Rru_A0061 0.38 +2.8
Or see this region's nucleotide sequence