Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0018 and Rru_A0019 are separated by 23 nucleotides Rru_A0019 and Rru_A0020 are separated by 98 nucleotides Rru_A0020 and Rru_A0021 overlap by 4 nucleotides
Rru_A0018: Rru_A0018 - Glutamate synthase, NADH/NADPH, small subunit 2 (NCBI), at 19,695 to 21,152
_A0018
Rru_A0019: Rru_A0019 - Glutamate synthase (ferredoxin) (NCBI), at 21,176 to 25,750
_A0019
Rru_A0020: Rru_A0020 - Glutathione peroxidase (NCBI), at 25,849 to 26,355
_A0020
Rru_A0021: Rru_A0021 - Transcriptional Regulator, MarR family (NCBI), at 26,352 to 26,888
_A0021
Position (kb) 21
22
23
24
25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 20.673 kb on - strand, within Rru_A0018 at 22.554 kb on - strand, within Rru_A0019 at 23.695 kb on + strand, within Rru_A0019 at 25.758 kb on + strand at 25.758 kb on + strand at 25.850 kb on + strand at 25.850 kb on + strand at 25.851 kb on - strand at 25.851 kb on - strand at 25.858 kb on + strand at 25.859 kb on - strand at 26.037 kb on - strand, within Rru_A0020 at 26.037 kb on - strand, within Rru_A0020 at 26.099 kb on + strand, within Rru_A0020 at 26.099 kb on + strand, within Rru_A0020 at 26.100 kb on - strand, within Rru_A0020 at 26.100 kb on - strand, within Rru_A0020 at 26.100 kb on - strand, within Rru_A0020 at 26.100 kb on - strand, within Rru_A0020 at 26.100 kb on - strand, within Rru_A0020 at 26.231 kb on + strand, within Rru_A0020 at 26.231 kb on + strand, within Rru_A0020 at 26.232 kb on - strand, within Rru_A0020 at 26.232 kb on - strand, within Rru_A0020 at 26.232 kb on - strand, within Rru_A0020 at 26.232 kb on - strand, within Rru_A0020 at 26.292 kb on - strand, within Rru_A0020 at 26.292 kb on - strand, within Rru_A0020 at 26.342 kb on + strand at 26.708 kb on - strand, within Rru_A0021
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 20,673 - Rru_A0018 0.67 -0.6 22,554 - Rru_A0019 0.30 -1.7 23,695 + Rru_A0019 0.55 +0.4 25,758 + +1.2 25,758 + +2.3 25,850 + +0.0 25,850 + +0.7 25,851 - -2.4 25,851 - -0.2 25,858 + -3.4 25,859 - +0.8 26,037 - Rru_A0020 0.37 -1.0 26,037 - Rru_A0020 0.37 -0.7 26,099 + Rru_A0020 0.49 -0.4 26,099 + Rru_A0020 0.49 -1.2 26,100 - Rru_A0020 0.50 +2.0 26,100 - Rru_A0020 0.50 -2.8 26,100 - Rru_A0020 0.50 -3.9 26,100 - Rru_A0020 0.50 +1.2 26,100 - Rru_A0020 0.50 +2.0 26,231 + Rru_A0020 0.75 -2.2 26,231 + Rru_A0020 0.75 +0.0 26,232 - Rru_A0020 0.76 -1.4 26,232 - Rru_A0020 0.76 +0.4 26,232 - Rru_A0020 0.76 +1.3 26,232 - Rru_A0020 0.76 -1.6 26,292 - Rru_A0020 0.87 -2.0 26,292 - Rru_A0020 0.87 -1.9 26,342 + +1.2 26,708 - Rru_A0021 0.66 +1.7
Or see this region's nucleotide sequence