Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0017 and Rru_A0018 are separated by 353 nucleotides Rru_A0018 and Rru_A0019 are separated by 23 nucleotides
Rru_A0017: Rru_A0017 - Bacitracin resistance protein BacA (NCBI), at 18,493 to 19,341
_A0017
Rru_A0018: Rru_A0018 - Glutamate synthase, NADH/NADPH, small subunit 2 (NCBI), at 19,695 to 21,152
_A0018
Rru_A0019: Rru_A0019 - Glutamate synthase (ferredoxin) (NCBI), at 21,176 to 25,750
_A0019
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.788 kb on + strand, within Rru_A0017 at 18.789 kb on - strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.201 kb on + strand, within Rru_A0017 at 19.361 kb on - strand at 19.379 kb on + strand at 19.428 kb on + strand at 19.432 kb on - strand at 19.470 kb on + strand at 19.470 kb on + strand at 19.471 kb on - strand at 19.471 kb on - strand at 19.471 kb on - strand at 19.471 kb on - strand at 19.477 kb on + strand at 19.662 kb on + strand at 19.662 kb on + strand at 19.662 kb on + strand at 19.936 kb on - strand, within Rru_A0018 at 20.673 kb on - strand, within Rru_A0018
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 18,788 + Rru_A0017 0.35 -1.9 18,789 - Rru_A0017 0.35 +0.4 18,793 + Rru_A0017 0.35 -3.1 18,793 + Rru_A0017 0.35 -0.6 18,794 - Rru_A0017 0.35 -1.0 18,794 - Rru_A0017 0.35 -1.4 19,151 + Rru_A0017 0.78 -2.1 19,151 + Rru_A0017 0.78 -1.3 19,152 - Rru_A0017 0.78 -1.8 19,152 - Rru_A0017 0.78 -0.5 19,201 + Rru_A0017 0.83 +0.4 19,361 - -2.2 19,379 + +0.0 19,428 + -1.5 19,432 - -1.9 19,470 + +0.7 19,470 + -1.2 19,471 - -1.7 19,471 - -1.3 19,471 - +0.2 19,471 - -1.3 19,477 + -3.0 19,662 + +0.6 19,662 + -2.7 19,662 + +1.0 19,936 - Rru_A0018 0.17 +2.0 20,673 - Rru_A0018 0.67 -0.6
Or see this region's nucleotide sequence