Experiment: carbon source anaerobic Succinic Acid 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0014 and Rru_A0015 are separated by 432 nucleotides Rru_A0015 and Rru_AR0001 are separated by 163 nucleotides Rru_AR0001 and Rru_A0016 are separated by 253 nucleotides
Rru_A0014: Rru_A0014 - KpsF/GutQ (NCBI), at 14,699 to 15,799
_A0014
Rru_A0015: Rru_A0015 - 3'-5' exonuclease (NCBI), at 16,232 to 16,852
_A0015
Rru_AR0001: Rru_AR0001 - tRNA-Leu (NCBI), at 17,016 to 17,102
_AR0001
Rru_A0016: Rru_A0016 - 3-beta-hydroxy-delta(5)-steroid dehydrogenase (NCBI), at 17,356 to 18,378
_A0016
Position (kb)
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 15.806 kb on - strand at 15.868 kb on + strand at 15.869 kb on - strand at 15.869 kb on - strand at 15.988 kb on - strand at 16.066 kb on - strand at 16.120 kb on + strand at 16.146 kb on + strand at 16.146 kb on + strand at 17.001 kb on + strand at 17.006 kb on + strand at 17.007 kb on - strand at 17.261 kb on + strand at 17.261 kb on + strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.741 kb on - strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source anaerobic Succinic Acid 31.25 mM remove 15,806 - +0.4 15,868 + +2.0 15,869 - -2.7 15,869 - -0.8 15,988 - -2.4 16,066 - -1.4 16,120 + +4.1 16,146 + +0.4 16,146 + +2.0 17,001 + +2.0 17,006 + +0.7 17,007 - +0.3 17,261 + +2.0 17,261 + +0.0 17,262 - -1.0 17,262 - +1.5 17,262 - +0.2 17,262 - +2.0 17,262 - -0.9 17,741 - Rru_A0016 0.38 -2.2 17,742 + Rru_A0016 0.38 +0.5 17,742 + Rru_A0016 0.38 +1.1 17,742 + Rru_A0016 0.38 +0.3 17,743 - Rru_A0016 0.38 +2.9 17,743 - Rru_A0016 0.38 -0.3
Or see this region's nucleotide sequence