Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_429

Experiment: Aluminum chloride 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_427 and Pf6N2E2_428 are separated by 125 nucleotidesPf6N2E2_428 and Pf6N2E2_429 are separated by 117 nucleotidesPf6N2E2_429 and Pf6N2E2_430 are separated by 185 nucleotides Pf6N2E2_427 - Conidiation-specific protein 10, at 495,464 to 495,643 _427 Pf6N2E2_428 - Protease subunit of ATP-dependent Clp proteases, at 495,769 to 496,332 _428 Pf6N2E2_429 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 496,450 to 497,505 _429 Pf6N2E2_430 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 497,691 to 498,770 _430 Position (kb) 496 497 498Strain fitness (log2 ratio) -2 -1 0 1 2at 495.689 kb on - strandat 495.689 kb on - strandat 495.770 kb on + strandat 495.771 kb on - strandat 495.771 kb on - strandat 495.818 kb on - strandat 495.906 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.990 kb on - strand, within Pf6N2E2_428at 495.990 kb on - strand, within Pf6N2E2_428at 496.031 kb on + strand, within Pf6N2E2_428at 496.032 kb on - strand, within Pf6N2E2_428at 496.097 kb on + strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.144 kb on + strand, within Pf6N2E2_428at 496.145 kb on - strand, within Pf6N2E2_428at 496.145 kb on - strand, within Pf6N2E2_428at 496.175 kb on + strand, within Pf6N2E2_428at 496.175 kb on + strand, within Pf6N2E2_428at 496.266 kb on + strand, within Pf6N2E2_428at 496.387 kb on + strandat 496.388 kb on - strandat 496.508 kb on - strandat 496.542 kb on + strandat 496.542 kb on + strandat 496.543 kb on - strandat 496.543 kb on - strandat 496.613 kb on + strand, within Pf6N2E2_429at 496.613 kb on + strand, within Pf6N2E2_429at 496.614 kb on - strand, within Pf6N2E2_429at 496.614 kb on - strand, within Pf6N2E2_429at 497.027 kb on + strand, within Pf6N2E2_429at 497.027 kb on + strand, within Pf6N2E2_429at 497.028 kb on - strand, within Pf6N2E2_429at 497.028 kb on - strand, within Pf6N2E2_429at 497.201 kb on + strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.247 kb on - strand, within Pf6N2E2_429at 497.322 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429at 497.341 kb on + strand, within Pf6N2E2_429at 497.403 kb on - strandat 497.459 kb on + strandat 497.460 kb on - strandat 497.460 kb on - strandat 497.693 kb on - strandat 497.693 kb on - strandat 497.982 kb on + strand, within Pf6N2E2_430at 497.982 kb on + strand, within Pf6N2E2_430at 497.983 kb on - strand, within Pf6N2E2_430at 498.025 kb on + strand, within Pf6N2E2_430at 498.025 kb on + strand, within Pf6N2E2_430at 498.026 kb on - strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strandat 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.250 kb on + strand, within Pf6N2E2_430

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 2 mM
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495,689 - +1.2
495,689 - -0.6
495,770 + -2.3
495,771 - -0.3
495,771 - +0.6
495,818 - +0.0
495,906 + Pf6N2E2_428 0.24 +0.1
495,944 + Pf6N2E2_428 0.31 +0.4
495,944 + Pf6N2E2_428 0.31 +0.3
495,944 + Pf6N2E2_428 0.31 +0.1
495,990 - Pf6N2E2_428 0.39 -0.7
495,990 - Pf6N2E2_428 0.39 -0.1
496,031 + Pf6N2E2_428 0.46 +0.2
496,032 - Pf6N2E2_428 0.47 -0.8
496,097 + Pf6N2E2_428 0.58 +2.7
496,098 - Pf6N2E2_428 0.58 -0.2
496,098 - Pf6N2E2_428 0.58 +0.8
496,098 - Pf6N2E2_428 0.58 +0.6
496,098 - Pf6N2E2_428 0.58 +0.3
496,098 - Pf6N2E2_428 0.58 +0.6
496,098 - Pf6N2E2_428 0.58 -0.0
496,144 + Pf6N2E2_428 0.66 +0.3
496,145 - Pf6N2E2_428 0.67 -0.6
496,145 - Pf6N2E2_428 0.67 -0.2
496,175 + Pf6N2E2_428 0.72 +0.5
496,175 + Pf6N2E2_428 0.72 +1.2
496,266 + Pf6N2E2_428 0.88 -1.2
496,387 + +1.2
496,388 - +0.8
496,508 - -0.3
496,542 + -0.9
496,542 + +0.3
496,543 - +0.1
496,543 - -0.5
496,613 + Pf6N2E2_429 0.15 +0.7
496,613 + Pf6N2E2_429 0.15 +0.1
496,614 - Pf6N2E2_429 0.16 -1.2
496,614 - Pf6N2E2_429 0.16 +0.7
497,027 + Pf6N2E2_429 0.55 +0.4
497,027 + Pf6N2E2_429 0.55 +0.4
497,028 - Pf6N2E2_429 0.55 +0.2
497,028 - Pf6N2E2_429 0.55 -0.6
497,201 + Pf6N2E2_429 0.71 -0.3
497,202 - Pf6N2E2_429 0.71 +0.3
497,202 - Pf6N2E2_429 0.71 -0.3
497,202 - Pf6N2E2_429 0.71 +0.1
497,202 - Pf6N2E2_429 0.71 -0.6
497,202 - Pf6N2E2_429 0.71 +1.3
497,247 - Pf6N2E2_429 0.75 +0.2
497,322 + Pf6N2E2_429 0.83 +0.3
497,324 + Pf6N2E2_429 0.83 +0.7
497,324 + Pf6N2E2_429 0.83 +0.2
497,324 + Pf6N2E2_429 0.83 -1.1
497,341 + Pf6N2E2_429 0.84 +1.1
497,403 - +0.0
497,459 + -0.7
497,460 - +0.0
497,460 - -2.3
497,693 - +0.8
497,693 - -1.0
497,982 + Pf6N2E2_430 0.27 -0.9
497,982 + Pf6N2E2_430 0.27 +0.7
497,983 - Pf6N2E2_430 0.27 -0.1
498,025 + Pf6N2E2_430 0.31 -0.3
498,025 + Pf6N2E2_430 0.31 -0.2
498,026 - Pf6N2E2_430 0.31 +0.9
498,208 + Pf6N2E2_430 0.48 -0.1
498,208 + -0.3
498,208 + Pf6N2E2_430 0.48 -0.6
498,208 + Pf6N2E2_430 0.48 +0.1
498,208 + Pf6N2E2_430 0.48 +1.7
498,208 + Pf6N2E2_430 0.48 -0.7
498,208 + Pf6N2E2_430 0.48 +0.7
498,209 - Pf6N2E2_430 0.48 +0.4
498,209 - Pf6N2E2_430 0.48 +0.4
498,209 - Pf6N2E2_430 0.48 -0.3
498,209 - Pf6N2E2_430 0.48 +0.7
498,209 - Pf6N2E2_430 0.48 +2.7
498,209 - Pf6N2E2_430 0.48 +0.7
498,250 + Pf6N2E2_430 0.52 -0.1

Or see this region's nucleotide sequence