Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5705 and Pf1N1B4_5706 overlap by 1 nucleotides Pf1N1B4_5706 and Pf1N1B4_5707 are separated by 10 nucleotides
Pf1N1B4_5705 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 142,638 to 144,539
_5705
Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660
_5706
Pf1N1B4_5707 - NADH dehydrogenase (EC 1.6.99.3), at 145,671 to 146,762
_5707
Position (kb) 144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 143.875 kb on + strand, within Pf1N1B4_5705 at 143.876 kb on - strand, within Pf1N1B4_5705 at 143.979 kb on + strand, within Pf1N1B4_5705 at 144.037 kb on + strand at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.180 kb on + strand at 144.181 kb on - strand, within Pf1N1B4_5705 at 144.278 kb on + strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.303 kb on + strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.362 kb on - strand at 144.362 kb on - strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.460 kb on + strand at 144.461 kb on - strand at 144.465 kb on - strand at 144.637 kb on - strand at 144.884 kb on + strand, within Pf1N1B4_5706 at 144.885 kb on - strand, within Pf1N1B4_5706 at 144.910 kb on + strand, within Pf1N1B4_5706 at 144.978 kb on + strand, within Pf1N1B4_5706 at 145.436 kb on + strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.484 kb on + strand, within Pf1N1B4_5706 at 145.502 kb on + strand, within Pf1N1B4_5706 at 145.639 kb on - strand at 145.847 kb on + strand, within Pf1N1B4_5707 at 146.047 kb on + strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand at 146.082 kb on - strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.151 kb on + strand, within Pf1N1B4_5707 at 146.152 kb on - strand, within Pf1N1B4_5707 at 146.190 kb on - strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.349 kb on - strand at 146.549 kb on - strand at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.582 kb on - strand, within Pf1N1B4_5707
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 143,875 + Pf1N1B4_5705 0.65 +0.7 143,876 - Pf1N1B4_5705 0.65 +1.2 143,979 + Pf1N1B4_5705 0.71 -0.6 144,037 + +1.3 144,038 - Pf1N1B4_5705 0.74 +0.5 144,038 - Pf1N1B4_5705 0.74 -0.6 144,180 + -1.3 144,181 - Pf1N1B4_5705 0.81 +1.3 144,278 + Pf1N1B4_5705 0.86 -2.9 144,279 - Pf1N1B4_5705 0.86 +0.2 144,279 - Pf1N1B4_5705 0.86 +0.3 144,303 + Pf1N1B4_5705 0.88 -0.3 144,304 - Pf1N1B4_5705 0.88 -0.6 144,304 - Pf1N1B4_5705 0.88 +1.5 144,362 - -0.3 144,362 - +2.2 144,373 + +2.0 144,373 + -0.3 144,373 + +0.0 144,460 + -0.7 144,461 - -0.3 144,465 - +0.8 144,637 - +0.2 144,884 + Pf1N1B4_5706 0.31 +0.7 144,885 - Pf1N1B4_5706 0.31 -1.3 144,910 + Pf1N1B4_5706 0.33 -0.5 144,978 + Pf1N1B4_5706 0.39 -0.5 145,436 + Pf1N1B4_5706 0.80 +1.1 145,479 - Pf1N1B4_5706 0.84 -1.9 145,479 - Pf1N1B4_5706 0.84 -0.3 145,484 + Pf1N1B4_5706 0.84 -2.9 145,502 + Pf1N1B4_5706 0.86 -0.9 145,639 - +0.3 145,847 + Pf1N1B4_5707 0.16 +0.4 146,047 + Pf1N1B4_5707 0.34 -0.3 146,048 - Pf1N1B4_5707 0.35 -1.9 146,048 - Pf1N1B4_5707 0.35 +0.1 146,048 - -0.3 146,082 - Pf1N1B4_5707 0.38 +1.9 146,132 + Pf1N1B4_5707 0.42 -0.3 146,132 + Pf1N1B4_5707 0.42 -1.6 146,132 + Pf1N1B4_5707 0.42 +0.7 146,133 - Pf1N1B4_5707 0.42 +0.4 146,133 - Pf1N1B4_5707 0.42 -0.0 146,151 + Pf1N1B4_5707 0.44 +0.9 146,152 - Pf1N1B4_5707 0.44 +1.5 146,190 - Pf1N1B4_5707 0.48 +0.5 146,348 + Pf1N1B4_5707 0.62 -0.3 146,348 + Pf1N1B4_5707 0.62 -0.3 146,349 - +2.0 146,549 - +0.7 146,555 - Pf1N1B4_5707 0.81 -1.6 146,555 - Pf1N1B4_5707 0.81 +0.0 146,582 - Pf1N1B4_5707 0.83 +1.0
Or see this region's nucleotide sequence