Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4748 and Pf1N1B4_4749 are separated by 246 nucleotides
Pf1N1B4_4748 - transporter, putative, at 5,243,344 to 5,244,531
_4748
Pf1N1B4_4749 - ABC transporter ATP-binding protein uup, at 5,244,778 to 5,246,343
_4749
Position (kb)
5244
5245
5246
5247 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.805 kb on - strand, within Pf1N1B4_4748 at 5243.814 kb on + strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.820 kb on + strand at 5243.821 kb on - strand, within Pf1N1B4_4748 at 5244.461 kb on + strand at 5244.461 kb on + strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.587 kb on + strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.807 kb on - strand at 5244.815 kb on + strand at 5244.815 kb on + strand at 5244.816 kb on - strand at 5245.209 kb on - strand, within Pf1N1B4_4749 at 5245.212 kb on - strand, within Pf1N1B4_4749 at 5245.649 kb on + strand, within Pf1N1B4_4749 at 5245.649 kb on + strand, within Pf1N1B4_4749 at 5245.649 kb on + strand, within Pf1N1B4_4749 at 5245.650 kb on - strand, within Pf1N1B4_4749 at 5245.658 kb on + strand, within Pf1N1B4_4749 at 5245.659 kb on - strand, within Pf1N1B4_4749 at 5246.060 kb on + strand, within Pf1N1B4_4749 at 5246.352 kb on + strand at 5246.370 kb on - strand at 5246.465 kb on + strand at 5246.561 kb on + strand at 5246.571 kb on + strand at 5246.571 kb on + strand at 5246.572 kb on - strand at 5246.696 kb on - strand at 5246.696 kb on - strand at 5246.702 kb on - strand at 5246.758 kb on + strand at 5246.876 kb on + strand at 5246.898 kb on - strand at 5246.952 kb on - strand at 5246.982 kb on - strand at 5247.072 kb on + strand at 5247.073 kb on - strand at 5247.073 kb on - strand at 5247.073 kb on - strand at 5247.085 kb on + strand at 5247.086 kb on - strand at 5247.086 kb on - strand at 5247.210 kb on + strand at 5247.211 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.320 kb on - strand at 5247.326 kb on + strand at 5247.327 kb on - strand at 5247.334 kb on - strand at 5247.334 kb on - strand at 5247.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,243,801 + Pf1N1B4_4748 0.38 -1.3 5,243,801 + Pf1N1B4_4748 0.38 +1.7 5,243,801 + Pf1N1B4_4748 0.38 -0.3 5,243,802 - Pf1N1B4_4748 0.39 +0.5 5,243,802 - Pf1N1B4_4748 0.39 +0.1 5,243,802 - Pf1N1B4_4748 0.39 +2.7 5,243,805 - Pf1N1B4_4748 0.39 -0.3 5,243,814 + Pf1N1B4_4748 0.40 +1.0 5,243,815 - Pf1N1B4_4748 0.40 -0.7 5,243,815 - Pf1N1B4_4748 0.40 +2.2 5,243,820 + +2.2 5,243,821 - Pf1N1B4_4748 0.40 -2.3 5,244,461 + +1.7 5,244,461 + -0.3 5,244,462 - -0.3 5,244,462 - -0.6 5,244,462 - +0.0 5,244,462 - +0.5 5,244,587 + +0.2 5,244,780 - -0.1 5,244,780 - +0.2 5,244,780 - +0.7 5,244,780 - -0.3 5,244,807 - +0.1 5,244,815 + -0.8 5,244,815 + -1.3 5,244,816 - -0.3 5,245,209 - Pf1N1B4_4749 0.28 -0.7 5,245,212 - Pf1N1B4_4749 0.28 +0.9 5,245,649 + Pf1N1B4_4749 0.56 +0.8 5,245,649 + Pf1N1B4_4749 0.56 -0.3 5,245,649 + Pf1N1B4_4749 0.56 -0.7 5,245,650 - Pf1N1B4_4749 0.56 +1.0 5,245,658 + Pf1N1B4_4749 0.56 -0.3 5,245,659 - Pf1N1B4_4749 0.56 +0.7 5,246,060 + Pf1N1B4_4749 0.82 +0.4 5,246,352 + +0.7 5,246,370 - +1.2 5,246,465 + +1.5 5,246,561 + -1.9 5,246,571 + -0.1 5,246,571 + -0.1 5,246,572 - +1.2 5,246,696 - -0.0 5,246,696 - +1.5 5,246,702 - +0.1 5,246,758 + +1.0 5,246,876 + +0.8 5,246,898 - -0.3 5,246,952 - -0.5 5,246,982 - -0.0 5,247,072 + +0.5 5,247,073 - -1.7 5,247,073 - +0.0 5,247,073 - -1.7 5,247,085 + +0.4 5,247,086 - +0.3 5,247,086 - -0.3 5,247,210 + -0.1 5,247,211 - -0.6 5,247,320 - +0.7 5,247,320 - +1.2 5,247,320 - +0.2 5,247,320 - +2.8 5,247,326 + -0.2 5,247,327 - +0.7 5,247,334 - +1.8 5,247,334 - +1.2 5,247,334 - -2.7
Or see this region's nucleotide sequence