Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotides Pf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides Pf1N1B4_4108 and Pf1N1B4_4109 are separated by 133 nucleotides Pf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotides
Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032
_4106
Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905
_4107
Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718
_4108
Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241
_4109
Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707
_4110
Position (kb)
4554
4555
4556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.057 kb on - strand at 4554.057 kb on - strand at 4554.091 kb on - strand at 4554.420 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.903 kb on + strand at 4554.935 kb on + strand at 4554.935 kb on + strand at 4554.974 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.450 kb on + strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.697 kb on - strand at 4555.761 kb on + strand at 4555.761 kb on + strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.767 kb on - strand at 4555.767 kb on - strand at 4555.786 kb on - strand at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.953 kb on - strand, within Pf1N1B4_4109 at 4555.953 kb on - strand at 4555.953 kb on - strand at 4555.962 kb on + strand, within Pf1N1B4_4109 at 4555.963 kb on - strand, within Pf1N1B4_4109 at 4555.985 kb on + strand, within Pf1N1B4_4109 at 4555.986 kb on - strand, within Pf1N1B4_4109 at 4556.062 kb on + strand at 4556.063 kb on - strand, within Pf1N1B4_4109 at 4556.068 kb on + strand, within Pf1N1B4_4109 at 4556.119 kb on - strand, within Pf1N1B4_4109 at 4556.125 kb on + strand, within Pf1N1B4_4109 at 4556.147 kb on + strand, within Pf1N1B4_4109 at 4556.216 kb on - strand at 4556.251 kb on + strand at 4556.378 kb on + strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,554,053 - +1.5 4,554,053 - +0.8 4,554,053 - +0.3 4,554,057 - -0.7 4,554,057 - +0.9 4,554,091 - -0.2 4,554,420 + Pf1N1B4_4107 0.23 -0.4 4,554,738 + Pf1N1B4_4107 0.73 +0.8 4,554,738 + Pf1N1B4_4107 0.73 -0.2 4,554,738 + Pf1N1B4_4107 0.73 -0.2 4,554,903 + +0.8 4,554,935 + -0.9 4,554,935 + +0.3 4,554,974 + +1.8 4,554,986 + -0.7 4,554,986 + -2.0 4,554,986 + -1.2 4,555,385 - Pf1N1B4_4108 0.58 +1.3 4,555,385 - Pf1N1B4_4108 0.58 +1.3 4,555,450 + Pf1N1B4_4108 0.66 +1.3 4,555,451 - Pf1N1B4_4108 0.66 -1.5 4,555,451 - Pf1N1B4_4108 0.66 -2.2 4,555,451 - -0.2 4,555,451 - Pf1N1B4_4108 0.66 +1.3 4,555,697 - -0.2 4,555,761 + -0.2 4,555,761 + -0.7 4,555,762 - +0.8 4,555,762 - -0.0 4,555,762 - +0.8 4,555,762 - +0.2 4,555,762 - +0.4 4,555,762 - -0.2 4,555,762 - -1.1 4,555,762 - -0.2 4,555,762 - -0.2 4,555,767 - -0.2 4,555,767 - +1.0 4,555,786 - +0.0 4,555,952 + Pf1N1B4_4109 0.26 -0.8 4,555,952 + Pf1N1B4_4109 0.26 +1.3 4,555,953 - Pf1N1B4_4109 0.26 -0.8 4,555,953 - -0.8 4,555,953 - -0.2 4,555,962 + Pf1N1B4_4109 0.28 +2.3 4,555,963 - Pf1N1B4_4109 0.28 -1.8 4,555,985 + Pf1N1B4_4109 0.34 -0.1 4,555,986 - Pf1N1B4_4109 0.34 +0.2 4,556,062 + +0.8 4,556,063 - Pf1N1B4_4109 0.54 -2.6 4,556,068 + Pf1N1B4_4109 0.55 +1.3 4,556,119 - Pf1N1B4_4109 0.68 -0.0 4,556,125 + Pf1N1B4_4109 0.70 -0.2 4,556,147 + Pf1N1B4_4109 0.76 +0.3 4,556,216 - -0.6 4,556,251 + +0.5 4,556,378 + +0.2 4,556,379 - -0.2 4,556,379 - -0.8 4,556,379 - +0.8 4,556,379 - +2.9 4,556,379 - +0.2 4,556,474 + Pf1N1B4_4110 0.30 +2.6 4,556,474 + Pf1N1B4_4110 0.30 -0.2 4,556,475 - Pf1N1B4_4110 0.31 +0.3 4,556,475 - Pf1N1B4_4110 0.31 +1.3
Or see this region's nucleotide sequence