Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213
_2477
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Position (kb)
2809
2810
2811 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2808.802 kb on + strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.901 kb on + strand, within Pf1N1B4_2476 at 2808.902 kb on - strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.114 kb on + strand at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.126 kb on - strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.334 kb on + strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.364 kb on + strand at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.657 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.749 kb on - strand, within Pf1N1B4_2478 at 2810.907 kb on + strand, within Pf1N1B4_2478 at 2810.972 kb on - strand, within Pf1N1B4_2478
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,808,802 + Pf1N1B4_2476 0.64 +0.6 2,808,803 - Pf1N1B4_2476 0.64 -1.9 2,808,803 - Pf1N1B4_2476 0.64 -0.3 2,808,803 - Pf1N1B4_2476 0.64 +1.1 2,808,803 - Pf1N1B4_2476 0.64 -0.2 2,808,803 - Pf1N1B4_2476 0.64 +0.9 2,808,901 + Pf1N1B4_2476 0.69 -0.5 2,808,902 - Pf1N1B4_2476 0.69 -0.0 2,809,104 + Pf1N1B4_2476 0.80 -0.2 2,809,104 + Pf1N1B4_2476 0.80 -0.7 2,809,114 + -0.3 2,809,125 + Pf1N1B4_2476 0.81 -0.6 2,809,125 + Pf1N1B4_2476 0.81 -0.3 2,809,126 - Pf1N1B4_2476 0.81 +0.9 2,809,280 + Pf1N1B4_2476 0.89 +1.1 2,809,280 + Pf1N1B4_2476 0.89 -0.6 2,809,281 - Pf1N1B4_2476 0.89 +1.8 2,809,281 - Pf1N1B4_2476 0.89 +1.3 2,809,281 - Pf1N1B4_2476 0.89 -1.9 2,809,334 + -0.9 2,809,335 - +0.5 2,809,335 - +1.5 2,809,335 - +0.1 2,809,364 + -0.7 2,809,411 + +0.3 2,809,412 - -0.9 2,809,412 - +0.9 2,809,412 - +0.1 2,809,489 - -1.2 2,809,587 + -0.5 2,809,587 + +2.9 2,809,587 + +1.0 2,809,587 + -0.7 2,809,587 + -1.2 2,809,587 + -2.5 2,809,587 + +0.8 2,809,587 + -1.2 2,809,588 - +0.1 2,809,588 - +0.4 2,809,588 - +1.1 2,809,588 - +0.2 2,809,588 - +0.6 2,809,593 + +0.7 2,809,593 + +1.1 2,809,593 + +0.1 2,809,594 - -0.7 2,809,594 - +2.0 2,809,594 - -0.7 2,809,594 - +1.3 2,809,594 - +0.2 2,809,594 - -1.3 2,809,594 - +1.0 2,809,594 - -0.5 2,809,594 - +0.1 2,809,597 + +0.2 2,809,598 - -0.2 2,809,598 - +1.1 2,809,598 - +0.4 2,809,619 - -0.6 2,809,656 + +1.1 2,809,656 + -0.6 2,809,657 - -1.5 2,809,657 - -2.2 2,809,775 + +0.9 2,809,775 + +1.3 2,809,776 - -0.3 2,809,954 + Pf1N1B4_2477 0.43 +0.7 2,809,954 + Pf1N1B4_2477 0.43 -1.5 2,809,954 + Pf1N1B4_2477 0.43 -1.4 2,809,954 + Pf1N1B4_2477 0.43 +1.1 2,810,128 + Pf1N1B4_2477 0.81 -1.7 2,810,128 + Pf1N1B4_2477 0.81 +0.7 2,810,128 + Pf1N1B4_2477 0.81 +0.2 2,810,129 - Pf1N1B4_2477 0.81 -0.9 2,810,385 - +0.1 2,810,657 - Pf1N1B4_2478 0.12 -0.3 2,810,657 - +1.7 2,810,657 - Pf1N1B4_2478 0.12 -1.5 2,810,749 - Pf1N1B4_2478 0.17 +0.1 2,810,907 + Pf1N1B4_2478 0.24 +0.6 2,810,972 - Pf1N1B4_2478 0.27 +1.1
Or see this region's nucleotide sequence