Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2296 and Pf1N1B4_2297 overlap by 1 nucleotides Pf1N1B4_2297 and Pf1N1B4_2298 are separated by 147 nucleotides
Pf1N1B4_2296 - FIG00350678: hypothetical protein, at 2,613,879 to 2,615,012
_2296
Pf1N1B4_2297 - FIG00954147: hypothetical protein, at 2,615,012 to 2,615,569
_2297
Pf1N1B4_2298 - probable integral membrane protein NMA1898, at 2,615,717 to 2,617,093
_2298
Position (kb)
2615
2616 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2614.140 kb on + strand, within Pf1N1B4_2296 at 2614.140 kb on + strand, within Pf1N1B4_2296 at 2614.255 kb on + strand, within Pf1N1B4_2296 at 2614.256 kb on - strand, within Pf1N1B4_2296 at 2614.258 kb on - strand, within Pf1N1B4_2296 at 2614.317 kb on + strand, within Pf1N1B4_2296 at 2614.317 kb on + strand, within Pf1N1B4_2296 at 2614.318 kb on - strand, within Pf1N1B4_2296 at 2614.350 kb on + strand, within Pf1N1B4_2296 at 2614.722 kb on + strand, within Pf1N1B4_2296 at 2614.723 kb on - strand, within Pf1N1B4_2296 at 2614.869 kb on + strand, within Pf1N1B4_2296 at 2614.982 kb on + strand at 2614.983 kb on - strand at 2614.983 kb on - strand at 2614.983 kb on - strand at 2614.996 kb on + strand at 2614.996 kb on + strand at 2614.996 kb on + strand at 2615.055 kb on - strand at 2615.078 kb on + strand, within Pf1N1B4_2297 at 2615.078 kb on + strand, within Pf1N1B4_2297 at 2615.264 kb on + strand, within Pf1N1B4_2297 at 2615.264 kb on + strand, within Pf1N1B4_2297 at 2615.264 kb on + strand, within Pf1N1B4_2297 at 2615.312 kb on + strand, within Pf1N1B4_2297 at 2615.312 kb on + strand, within Pf1N1B4_2297 at 2615.312 kb on + strand, within Pf1N1B4_2297 at 2615.360 kb on + strand, within Pf1N1B4_2297 at 2615.361 kb on - strand, within Pf1N1B4_2297 at 2615.567 kb on + strand at 2615.630 kb on + strand at 2615.630 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.636 kb on + strand at 2615.637 kb on - strand at 2615.637 kb on - strand at 2615.637 kb on - strand at 2615.637 kb on - strand at 2615.921 kb on + strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2615.922 kb on - strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.256 kb on - strand, within Pf1N1B4_2298 at 2616.386 kb on + strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,614,140 + Pf1N1B4_2296 0.23 -1.8 2,614,140 + Pf1N1B4_2296 0.23 -0.0 2,614,255 + Pf1N1B4_2296 0.33 +0.5 2,614,256 - Pf1N1B4_2296 0.33 -0.2 2,614,258 - Pf1N1B4_2296 0.33 +0.2 2,614,317 + Pf1N1B4_2296 0.39 -0.5 2,614,317 + Pf1N1B4_2296 0.39 +3.2 2,614,318 - Pf1N1B4_2296 0.39 -0.5 2,614,350 + Pf1N1B4_2296 0.42 -0.4 2,614,722 + Pf1N1B4_2296 0.74 -0.6 2,614,723 - Pf1N1B4_2296 0.74 +1.4 2,614,869 + Pf1N1B4_2296 0.87 +2.2 2,614,982 + -2.0 2,614,983 - -1.3 2,614,983 - +0.2 2,614,983 - -2.4 2,614,996 + -1.3 2,614,996 + -0.2 2,614,996 + -0.5 2,615,055 - -1.1 2,615,078 + Pf1N1B4_2297 0.12 +1.5 2,615,078 + Pf1N1B4_2297 0.12 -0.0 2,615,264 + Pf1N1B4_2297 0.45 -1.1 2,615,264 + Pf1N1B4_2297 0.45 +2.1 2,615,264 + Pf1N1B4_2297 0.45 +0.2 2,615,312 + Pf1N1B4_2297 0.54 -0.8 2,615,312 + Pf1N1B4_2297 0.54 +0.9 2,615,312 + Pf1N1B4_2297 0.54 +0.4 2,615,360 + Pf1N1B4_2297 0.62 +0.8 2,615,361 - Pf1N1B4_2297 0.63 +2.0 2,615,567 + -0.2 2,615,630 + -0.4 2,615,630 + +0.2 2,615,636 + +1.4 2,615,636 + +1.2 2,615,636 + +0.8 2,615,636 + +0.7 2,615,636 + +1.8 2,615,636 + -1.4 2,615,636 + -2.1 2,615,637 - +0.9 2,615,637 - -0.6 2,615,637 - +0.2 2,615,637 - +0.2 2,615,921 + Pf1N1B4_2298 0.15 -0.8 2,615,922 - Pf1N1B4_2298 0.15 +0.9 2,615,922 - Pf1N1B4_2298 0.15 +0.8 2,615,922 - Pf1N1B4_2298 0.15 +1.0 2,615,922 - Pf1N1B4_2298 0.15 -0.4 2,615,922 - Pf1N1B4_2298 0.15 -0.3 2,615,922 - Pf1N1B4_2298 0.15 +0.2 2,616,255 + Pf1N1B4_2298 0.39 +1.0 2,616,255 + Pf1N1B4_2298 0.39 -0.8 2,616,255 + Pf1N1B4_2298 0.39 -0.9 2,616,255 + Pf1N1B4_2298 0.39 +0.5 2,616,255 + Pf1N1B4_2298 0.39 -0.6 2,616,256 - Pf1N1B4_2298 0.39 -0.6 2,616,386 + Pf1N1B4_2298 0.49 -1.8 2,616,387 - Pf1N1B4_2298 0.49 +0.9 2,616,387 - Pf1N1B4_2298 0.49 -0.1 2,616,387 - Pf1N1B4_2298 0.49 -1.8 2,616,504 + Pf1N1B4_2298 0.57 -1.4 2,616,504 + Pf1N1B4_2298 0.57 -0.4 2,616,504 + Pf1N1B4_2298 0.57 +2.2
Or see this region's nucleotide sequence