Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_218

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_217 and Pf1N1B4_218 are separated by 112 nucleotidesPf1N1B4_218 and Pf1N1B4_219 are separated by 144 nucleotidesPf1N1B4_219 and Pf1N1B4_220 are separated by 178 nucleotides Pf1N1B4_217 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 276,453 to 277,466 _217 Pf1N1B4_218 - Periplasmic binding protein, at 277,579 to 278,322 _218 Pf1N1B4_219 - Acyclic terpenes utilization regulator AtuR, TetR family, at 278,467 to 279,093 _219 Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074 _220 Position (kb) 277 278 279Strain fitness (log2 ratio) -2 -1 0 1 2at 276.640 kb on + strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.775 kb on + strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.787 kb on + strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.831 kb on - strandat 276.831 kb on - strand, within Pf1N1B4_217at 276.860 kb on - strand, within Pf1N1B4_217at 276.961 kb on - strand, within Pf1N1B4_217at 277.081 kb on + strand, within Pf1N1B4_217at 277.588 kb on - strandat 277.614 kb on - strandat 277.614 kb on - strandat 277.725 kb on - strand, within Pf1N1B4_218at 277.725 kb on - strand, within Pf1N1B4_218at 277.799 kb on + strand, within Pf1N1B4_218at 277.799 kb on + strand, within Pf1N1B4_218at 277.800 kb on - strand, within Pf1N1B4_218at 277.868 kb on + strand, within Pf1N1B4_218at 277.868 kb on + strand, within Pf1N1B4_218at 277.869 kb on - strand, within Pf1N1B4_218at 277.869 kb on - strand, within Pf1N1B4_218at 277.869 kb on - strand, within Pf1N1B4_218at 277.886 kb on + strand, within Pf1N1B4_218at 277.886 kb on + strandat 277.886 kb on + strand, within Pf1N1B4_218at 277.886 kb on + strand, within Pf1N1B4_218at 277.887 kb on - strandat 277.887 kb on - strand, within Pf1N1B4_218at 277.887 kb on - strand, within Pf1N1B4_218at 277.887 kb on - strandat 277.887 kb on - strandat 277.902 kb on - strand, within Pf1N1B4_218at 277.902 kb on - strand, within Pf1N1B4_218at 277.902 kb on - strandat 277.935 kb on - strand, within Pf1N1B4_218at 277.985 kb on + strand, within Pf1N1B4_218at 277.985 kb on + strand, within Pf1N1B4_218at 277.986 kb on - strand, within Pf1N1B4_218at 277.986 kb on - strand, within Pf1N1B4_218at 277.986 kb on - strand, within Pf1N1B4_218at 278.255 kb on + strandat 278.256 kb on - strandat 278.256 kb on - strandat 278.385 kb on - strandat 278.385 kb on - strandat 278.440 kb on - strandat 278.660 kb on + strand, within Pf1N1B4_219at 278.661 kb on - strand, within Pf1N1B4_219at 278.664 kb on + strand, within Pf1N1B4_219at 278.720 kb on + strand, within Pf1N1B4_219at 278.721 kb on - strand, within Pf1N1B4_219at 278.721 kb on - strandat 278.721 kb on - strand, within Pf1N1B4_219at 278.849 kb on + strand, within Pf1N1B4_219at 278.914 kb on - strand, within Pf1N1B4_219at 278.969 kb on + strand, within Pf1N1B4_219at 279.106 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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276,640 + Pf1N1B4_217 0.18 -0.0
276,641 - Pf1N1B4_217 0.19 -0.5
276,641 - Pf1N1B4_217 0.19 -0.4
276,743 - Pf1N1B4_217 0.29 -0.7
276,743 - Pf1N1B4_217 0.29 -0.1
276,743 - +2.5
276,743 - Pf1N1B4_217 0.29 +2.2
276,743 - -1.1
276,743 - Pf1N1B4_217 0.29 -0.9
276,775 + Pf1N1B4_217 0.32 -2.1
276,776 - Pf1N1B4_217 0.32 +0.1
276,776 - Pf1N1B4_217 0.32 -1.2
276,787 + Pf1N1B4_217 0.33 +0.3
276,788 - Pf1N1B4_217 0.33 -0.9
276,788 - Pf1N1B4_217 0.33 +1.5
276,831 - +0.9
276,831 - Pf1N1B4_217 0.37 -1.4
276,860 - Pf1N1B4_217 0.40 +0.1
276,961 - Pf1N1B4_217 0.50 +0.1
277,081 + Pf1N1B4_217 0.62 +0.3
277,588 - +0.7
277,614 - +2.3
277,614 - +0.4
277,725 - Pf1N1B4_218 0.20 +1.5
277,725 - Pf1N1B4_218 0.20 +1.5
277,799 + Pf1N1B4_218 0.30 -0.1
277,799 + Pf1N1B4_218 0.30 +0.8
277,800 - Pf1N1B4_218 0.30 +0.1
277,868 + Pf1N1B4_218 0.39 +0.9
277,868 + Pf1N1B4_218 0.39 +1.5
277,869 - Pf1N1B4_218 0.39 +1.5
277,869 - Pf1N1B4_218 0.39 +0.5
277,869 - Pf1N1B4_218 0.39 +1.2
277,886 + Pf1N1B4_218 0.41 +0.5
277,886 + -0.1
277,886 + Pf1N1B4_218 0.41 +0.7
277,886 + Pf1N1B4_218 0.41 +0.1
277,887 - -0.5
277,887 - Pf1N1B4_218 0.41 +0.3
277,887 - Pf1N1B4_218 0.41 -0.3
277,887 - +2.5
277,887 - +0.9
277,902 - Pf1N1B4_218 0.43 +0.4
277,902 - Pf1N1B4_218 0.43 +0.6
277,902 - +0.9
277,935 - Pf1N1B4_218 0.48 +1.3
277,985 + Pf1N1B4_218 0.55 +0.5
277,985 + Pf1N1B4_218 0.55 +2.2
277,986 - Pf1N1B4_218 0.55 -0.6
277,986 - Pf1N1B4_218 0.55 +1.0
277,986 - Pf1N1B4_218 0.55 +1.8
278,255 + +0.7
278,256 - -0.7
278,256 - +1.3
278,385 - -0.3
278,385 - +0.3
278,440 - +0.7
278,660 + Pf1N1B4_219 0.31 -0.9
278,661 - Pf1N1B4_219 0.31 +0.5
278,664 + Pf1N1B4_219 0.31 -1.1
278,720 + Pf1N1B4_219 0.40 +0.7
278,721 - Pf1N1B4_219 0.41 +0.6
278,721 - -0.1
278,721 - Pf1N1B4_219 0.41 +1.0
278,849 + Pf1N1B4_219 0.61 -0.1
278,914 - Pf1N1B4_219 0.71 +0.4
278,969 + Pf1N1B4_219 0.80 -0.7
279,106 - -0.1

Or see this region's nucleotide sequence