Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_18

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_17 and Pf1N1B4_18 are separated by 103 nucleotidesPf1N1B4_18 and Pf1N1B4_19 are separated by 120 nucleotides Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855 _17 Pf1N1B4_18 - 16 kDa heat shock protein A, at 21,959 to 22,405 _18 Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485 _19 Position (kb) 21 22 23Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 21.055 kb on + strand, within Pf1N1B4_17at 21.055 kb on + strand, within Pf1N1B4_17at 21.056 kb on - strand, within Pf1N1B4_17at 21.056 kb on - strand, within Pf1N1B4_17at 21.066 kb on + strand, within Pf1N1B4_17at 21.066 kb on + strand, within Pf1N1B4_17at 21.067 kb on - strandat 21.067 kb on - strand, within Pf1N1B4_17at 21.204 kb on - strand, within Pf1N1B4_17at 21.598 kb on + strandat 21.598 kb on + strandat 21.794 kb on - strandat 21.794 kb on - strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.853 kb on + strandat 21.885 kb on + strandat 21.893 kb on + strandat 21.893 kb on + strandat 22.141 kb on - strand, within Pf1N1B4_18at 22.141 kb on - strand, within Pf1N1B4_18at 22.141 kb on - strand, within Pf1N1B4_18at 22.146 kb on + strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.290 kb on + strand, within Pf1N1B4_18at 22.291 kb on - strand, within Pf1N1B4_18at 22.299 kb on + strand, within Pf1N1B4_18at 22.300 kb on - strand, within Pf1N1B4_18at 22.300 kb on - strand, within Pf1N1B4_18at 22.400 kb on - strandat 22.400 kb on - strandat 22.425 kb on + strandat 22.472 kb on - strandat 22.483 kb on - strandat 22.483 kb on - strandat 22.483 kb on - strandat 22.556 kb on + strandat 22.623 kb on + strand, within Pf1N1B4_19at 22.959 kb on + strand, within Pf1N1B4_19at 22.959 kb on + strand, within Pf1N1B4_19at 23.349 kb on + strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.362 kb on + strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.386 kb on + strand, within Pf1N1B4_19

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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21,055 + Pf1N1B4_17 0.75 +2.7
21,055 + Pf1N1B4_17 0.75 +0.9
21,056 - Pf1N1B4_17 0.75 +0.3
21,056 - Pf1N1B4_17 0.75 +1.0
21,066 + Pf1N1B4_17 0.76 +1.9
21,066 + Pf1N1B4_17 0.76 +1.3
21,067 - +1.5
21,067 - Pf1N1B4_17 0.76 -2.4
21,204 - Pf1N1B4_17 0.80 +0.5
21,598 + -0.2
21,598 + +1.2
21,794 - +1.7
21,794 - +1.3
21,802 + +1.5
21,802 + +1.9
21,802 + -1.7
21,802 + +0.6
21,853 + +0.1
21,885 + -2.9
21,893 + -0.1
21,893 + +0.7
22,141 - Pf1N1B4_18 0.41 +1.3
22,141 - Pf1N1B4_18 0.41 +1.6
22,141 - Pf1N1B4_18 0.41 +0.7
22,146 + Pf1N1B4_18 0.42 +0.3
22,147 - Pf1N1B4_18 0.42 +0.6
22,147 - Pf1N1B4_18 0.42 +2.2
22,147 - Pf1N1B4_18 0.42 +0.7
22,147 - Pf1N1B4_18 0.42 +1.1
22,147 - Pf1N1B4_18 0.42 +0.9
22,147 - Pf1N1B4_18 0.42 +0.9
22,147 - Pf1N1B4_18 0.42 -0.7
22,147 - Pf1N1B4_18 0.42 +1.2
22,147 - Pf1N1B4_18 0.42 +1.5
22,147 - Pf1N1B4_18 0.42 +0.3
22,147 - Pf1N1B4_18 0.42 -0.5
22,290 + Pf1N1B4_18 0.74 +0.9
22,291 - Pf1N1B4_18 0.74 +1.5
22,299 + Pf1N1B4_18 0.76 +1.9
22,300 - Pf1N1B4_18 0.76 -2.4
22,300 - Pf1N1B4_18 0.76 -0.8
22,400 - +1.2
22,400 - -2.7
22,425 + +0.2
22,472 - -0.7
22,483 - +0.9
22,483 - -0.1
22,483 - +0.9
22,556 + +1.1
22,623 + Pf1N1B4_19 0.10 -0.9
22,959 + Pf1N1B4_19 0.45 -0.7
22,959 + Pf1N1B4_19 0.45 -0.5
23,349 + Pf1N1B4_19 0.86 +0.0
23,353 - Pf1N1B4_19 0.86 -0.4
23,353 - Pf1N1B4_19 0.86 -1.5
23,362 + Pf1N1B4_19 0.87 +0.7
23,363 - Pf1N1B4_19 0.87 +1.2
23,363 - Pf1N1B4_19 0.87 +0.6
23,386 + Pf1N1B4_19 0.90 +0.3

Or see this region's nucleotide sequence