Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_17 and Pf1N1B4_18 are separated by 103 nucleotides Pf1N1B4_18 and Pf1N1B4_19 are separated by 120 nucleotides
Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855
_17
Pf1N1B4_18 - 16 kDa heat shock protein A, at 21,959 to 22,405
_18
Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485
_19
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 21.055 kb on + strand, within Pf1N1B4_17 at 21.055 kb on + strand, within Pf1N1B4_17 at 21.056 kb on - strand, within Pf1N1B4_17 at 21.056 kb on - strand, within Pf1N1B4_17 at 21.066 kb on + strand, within Pf1N1B4_17 at 21.066 kb on + strand, within Pf1N1B4_17 at 21.067 kb on - strand at 21.067 kb on - strand, within Pf1N1B4_17 at 21.204 kb on - strand, within Pf1N1B4_17 at 21.598 kb on + strand at 21.598 kb on + strand at 21.794 kb on - strand at 21.794 kb on - strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.802 kb on + strand at 21.853 kb on + strand at 21.885 kb on + strand at 21.893 kb on + strand at 21.893 kb on + strand at 22.141 kb on - strand, within Pf1N1B4_18 at 22.141 kb on - strand, within Pf1N1B4_18 at 22.141 kb on - strand, within Pf1N1B4_18 at 22.146 kb on + strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.147 kb on - strand, within Pf1N1B4_18 at 22.290 kb on + strand, within Pf1N1B4_18 at 22.291 kb on - strand, within Pf1N1B4_18 at 22.299 kb on + strand, within Pf1N1B4_18 at 22.300 kb on - strand, within Pf1N1B4_18 at 22.300 kb on - strand, within Pf1N1B4_18 at 22.400 kb on - strand at 22.400 kb on - strand at 22.425 kb on + strand at 22.472 kb on - strand at 22.483 kb on - strand at 22.483 kb on - strand at 22.483 kb on - strand at 22.556 kb on + strand at 22.623 kb on + strand, within Pf1N1B4_19 at 22.959 kb on + strand, within Pf1N1B4_19 at 22.959 kb on + strand, within Pf1N1B4_19 at 23.349 kb on + strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.362 kb on + strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.386 kb on + strand, within Pf1N1B4_19
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 21,055 + Pf1N1B4_17 0.75 +2.7 21,055 + Pf1N1B4_17 0.75 +0.9 21,056 - Pf1N1B4_17 0.75 +0.3 21,056 - Pf1N1B4_17 0.75 +1.0 21,066 + Pf1N1B4_17 0.76 +1.9 21,066 + Pf1N1B4_17 0.76 +1.3 21,067 - +1.5 21,067 - Pf1N1B4_17 0.76 -2.4 21,204 - Pf1N1B4_17 0.80 +0.5 21,598 + -0.2 21,598 + +1.2 21,794 - +1.7 21,794 - +1.3 21,802 + +1.5 21,802 + +1.9 21,802 + -1.7 21,802 + +0.6 21,853 + +0.1 21,885 + -2.9 21,893 + -0.1 21,893 + +0.7 22,141 - Pf1N1B4_18 0.41 +1.3 22,141 - Pf1N1B4_18 0.41 +1.6 22,141 - Pf1N1B4_18 0.41 +0.7 22,146 + Pf1N1B4_18 0.42 +0.3 22,147 - Pf1N1B4_18 0.42 +0.6 22,147 - Pf1N1B4_18 0.42 +2.2 22,147 - Pf1N1B4_18 0.42 +0.7 22,147 - Pf1N1B4_18 0.42 +1.1 22,147 - Pf1N1B4_18 0.42 +0.9 22,147 - Pf1N1B4_18 0.42 +0.9 22,147 - Pf1N1B4_18 0.42 -0.7 22,147 - Pf1N1B4_18 0.42 +1.2 22,147 - Pf1N1B4_18 0.42 +1.5 22,147 - Pf1N1B4_18 0.42 +0.3 22,147 - Pf1N1B4_18 0.42 -0.5 22,290 + Pf1N1B4_18 0.74 +0.9 22,291 - Pf1N1B4_18 0.74 +1.5 22,299 + Pf1N1B4_18 0.76 +1.9 22,300 - Pf1N1B4_18 0.76 -2.4 22,300 - Pf1N1B4_18 0.76 -0.8 22,400 - +1.2 22,400 - -2.7 22,425 + +0.2 22,472 - -0.7 22,483 - +0.9 22,483 - -0.1 22,483 - +0.9 22,556 + +1.1 22,623 + Pf1N1B4_19 0.10 -0.9 22,959 + Pf1N1B4_19 0.45 -0.7 22,959 + Pf1N1B4_19 0.45 -0.5 23,349 + Pf1N1B4_19 0.86 +0.0 23,353 - Pf1N1B4_19 0.86 -0.4 23,353 - Pf1N1B4_19 0.86 -1.5 23,362 + Pf1N1B4_19 0.87 +0.7 23,363 - Pf1N1B4_19 0.87 +1.2 23,363 - Pf1N1B4_19 0.87 +0.6 23,386 + Pf1N1B4_19 0.90 +0.3
Or see this region's nucleotide sequence