Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_127 and Pf1N1B4_128 are separated by 254 nucleotides Pf1N1B4_128 and Pf1N1B4_129 are separated by 3 nucleotides
Pf1N1B4_127 - FIG032225: Transcriptional regulator, LysR family, at 138,939 to 139,847
_127
Pf1N1B4_128 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 140,102 to 140,905
_128
Pf1N1B4_129 - sensory box histidine kinase/response regulator, at 140,909 to 144,151
_129
Position (kb)
140
141 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.294 kb on - strand, within Pf1N1B4_127 at 139.498 kb on + strand, within Pf1N1B4_127 at 139.542 kb on + strand, within Pf1N1B4_127 at 140.007 kb on + strand at 140.063 kb on + strand at 140.129 kb on - strand at 140.521 kb on + strand, within Pf1N1B4_128 at 140.522 kb on - strand, within Pf1N1B4_128 at 140.574 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.957 kb on - strand at 140.957 kb on - strand at 140.957 kb on - strand at 141.353 kb on - strand, within Pf1N1B4_129 at 141.353 kb on - strand, within Pf1N1B4_129 at 141.490 kb on + strand, within Pf1N1B4_129
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 139,134 + Pf1N1B4_127 0.21 +1.5 139,134 + Pf1N1B4_127 0.21 +1.2 139,281 + Pf1N1B4_127 0.38 -1.1 139,281 + Pf1N1B4_127 0.38 -0.1 139,282 - Pf1N1B4_127 0.38 -0.6 139,282 - Pf1N1B4_127 0.38 -0.1 139,282 - Pf1N1B4_127 0.38 -3.1 139,282 - Pf1N1B4_127 0.38 -1.1 139,293 + Pf1N1B4_127 0.39 -0.7 139,293 + Pf1N1B4_127 0.39 -0.1 139,294 - Pf1N1B4_127 0.39 -0.4 139,498 + Pf1N1B4_127 0.61 +0.2 139,542 + Pf1N1B4_127 0.66 -1.1 140,007 + -1.7 140,063 + +0.2 140,129 - -0.3 140,521 + Pf1N1B4_128 0.52 +1.2 140,522 - Pf1N1B4_128 0.52 -0.1 140,574 - Pf1N1B4_128 0.59 -0.6 140,634 - Pf1N1B4_128 0.66 +0.9 140,634 - Pf1N1B4_128 0.66 +0.7 140,634 - Pf1N1B4_128 0.66 +1.1 140,634 - Pf1N1B4_128 0.66 +1.0 140,956 + +0.2 140,956 + +1.7 140,956 + +0.3 140,956 + +1.2 140,956 + -0.1 140,957 - -1.1 140,957 - +0.4 140,957 - +0.3 141,353 - Pf1N1B4_129 0.14 -0.6 141,353 - Pf1N1B4_129 0.14 +1.2 141,490 + Pf1N1B4_129 0.18 -0.1
Or see this region's nucleotide sequence