Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04360 and MMJJ_RS04365 are separated by 690 nucleotides MMJJ_RS04365 and MMJJ_RS04370 are separated by 447 nucleotides
MMJJ_RS04360: MMJJ_RS04360 - winged helix-turn-helix transcriptional regulator, at 812,567 to 812,869
_RS04360
MMJJ_RS04365: MMJJ_RS04365 - helix-turn-helix transcriptional regulator, at 813,560 to 813,838
_RS04365
MMJJ_RS04370: MMJJ_RS04370 - SIR2 family protein, at 814,286 to 815,947
_RS04370
Position (kb) 813
814 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 812.563 kb on - strand at 812.563 kb on - strand at 812.567 kb on + strand at 812.567 kb on + strand at 812.567 kb on + strand at 812.637 kb on - strand, within MMJJ_RS04360 at 812.657 kb on + strand, within MMJJ_RS04360 at 812.660 kb on - strand, within MMJJ_RS04360 at 812.660 kb on - strand, within MMJJ_RS04360 at 812.722 kb on + strand, within MMJJ_RS04360 at 812.768 kb on - strand, within MMJJ_RS04360 at 812.772 kb on + strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.836 kb on - strand, within MMJJ_RS04360 at 812.836 kb on - strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.840 kb on - strand at 812.841 kb on + strand at 812.841 kb on + strand at 812.857 kb on - strand at 812.889 kb on + strand at 812.895 kb on - strand at 812.917 kb on - strand at 812.917 kb on - strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.162 kb on + strand at 813.169 kb on + strand at 813.169 kb on + strand at 813.216 kb on - strand at 813.216 kb on - strand at 813.217 kb on + strand at 813.219 kb on - strand at 813.235 kb on + strand at 813.269 kb on - strand at 813.270 kb on + strand at 813.270 kb on + strand at 813.293 kb on + strand at 813.306 kb on - strand at 813.306 kb on - strand at 813.307 kb on + strand at 813.311 kb on + strand at 813.330 kb on + strand at 813.345 kb on - strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.353 kb on + strand at 813.354 kb on - strand at 813.354 kb on - strand at 813.355 kb on + strand at 813.355 kb on + strand at 813.366 kb on + strand at 813.379 kb on - strand at 813.413 kb on + strand at 813.416 kb on - strand at 813.432 kb on - strand at 813.448 kb on + strand at 813.448 kb on + strand at 813.457 kb on - strand at 813.464 kb on - strand at 813.465 kb on + strand at 813.566 kb on + strand at 813.568 kb on - strand at 813.588 kb on - strand, within MMJJ_RS04365 at 813.591 kb on - strand, within MMJJ_RS04365 at 813.591 kb on - strand, within MMJJ_RS04365 at 813.592 kb on + strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.598 kb on + strand, within MMJJ_RS04365 at 813.598 kb on + strand, within MMJJ_RS04365 at 813.633 kb on - strand, within MMJJ_RS04365 at 813.637 kb on - strand, within MMJJ_RS04365 at 813.637 kb on - strand, within MMJJ_RS04365 at 813.638 kb on + strand, within MMJJ_RS04365 at 813.641 kb on + strand, within MMJJ_RS04365 at 813.667 kb on - strand, within MMJJ_RS04365 at 813.690 kb on - strand, within MMJJ_RS04365 at 813.691 kb on + strand, within MMJJ_RS04365 at 813.701 kb on + strand, within MMJJ_RS04365 at 813.821 kb on - strand at 813.822 kb on + strand at 813.826 kb on - strand at 813.826 kb on - strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.837 kb on + strand at 813.839 kb on - strand at 813.839 kb on - strand at 813.840 kb on + strand at 813.840 kb on + strand at 813.840 kb on + strand at 813.845 kb on + strand at 813.847 kb on - strand at 813.871 kb on - strand at 813.877 kb on - strand at 813.878 kb on + strand at 813.878 kb on + strand at 813.878 kb on + strand at 813.880 kb on - strand at 813.881 kb on + strand at 813.881 kb on + strand at 813.881 kb on + strand at 813.881 kb on + strand at 813.881 kb on + strand at 813.884 kb on - strand at 813.891 kb on - strand at 813.898 kb on + strand at 813.898 kb on + strand at 813.899 kb on - strand at 813.913 kb on - strand at 814.040 kb on - strand at 814.052 kb on - strand at 814.069 kb on + strand at 814.069 kb on + strand at 814.154 kb on + strand at 814.158 kb on + strand at 814.184 kb on - strand at 814.184 kb on - strand at 814.184 kb on - strand at 814.184 kb on - strand at 814.185 kb on + strand at 814.185 kb on + strand at 814.185 kb on + strand at 814.186 kb on - strand at 814.189 kb on + strand at 814.189 kb on + strand at 814.213 kb on - strand at 814.254 kb on - strand at 814.271 kb on - strand at 814.312 kb on - strand at 814.312 kb on - strand at 814.331 kb on - strand at 814.360 kb on + strand at 814.381 kb on - strand at 814.382 kb on + strand at 814.382 kb on + strand at 814.385 kb on + strand at 814.398 kb on - strand at 814.400 kb on - strand at 814.401 kb on + strand at 814.401 kb on + strand at 814.429 kb on - strand at 814.429 kb on - strand at 814.430 kb on + strand at 814.431 kb on - strand at 814.431 kb on - strand at 814.431 kb on - strand at 814.432 kb on + strand at 814.432 kb on + strand at 814.432 kb on + strand at 814.510 kb on - strand, within MMJJ_RS04370 at 814.517 kb on - strand, within MMJJ_RS04370 at 814.543 kb on + strand, within MMJJ_RS04370 at 814.543 kb on + strand, within MMJJ_RS04370 at 814.564 kb on - strand, within MMJJ_RS04370 at 814.564 kb on - strand, within MMJJ_RS04370 at 814.566 kb on - strand, within MMJJ_RS04370 at 814.567 kb on + strand, within MMJJ_RS04370 at 814.573 kb on + strand, within MMJJ_RS04370 at 814.573 kb on + strand, within MMJJ_RS04370 at 814.573 kb on + strand, within MMJJ_RS04370 at 814.573 kb on + strand, within MMJJ_RS04370 at 814.575 kb on + strand, within MMJJ_RS04370 at 814.596 kb on - strand, within MMJJ_RS04370 at 814.604 kb on - strand, within MMJJ_RS04370 at 814.638 kb on - strand, within MMJJ_RS04370 at 814.679 kb on + strand, within MMJJ_RS04370 at 814.693 kb on - strand, within MMJJ_RS04370 at 814.694 kb on + strand, within MMJJ_RS04370 at 814.694 kb on + strand, within MMJJ_RS04370 at 814.741 kb on - strand, within MMJJ_RS04370 at 814.750 kb on - strand, within MMJJ_RS04370 at 814.750 kb on - strand, within MMJJ_RS04370 at 814.751 kb on + strand, within MMJJ_RS04370 at 814.751 kb on + strand, within MMJJ_RS04370 at 814.751 kb on + strand, within MMJJ_RS04370 at 814.752 kb on - strand, within MMJJ_RS04370 at 814.752 kb on - strand, within MMJJ_RS04370 at 814.752 kb on - strand, within MMJJ_RS04370 at 814.753 kb on + strand, within MMJJ_RS04370 at 814.753 kb on + strand, within MMJJ_RS04370 at 814.759 kb on - strand, within MMJJ_RS04370 at 814.759 kb on - strand, within MMJJ_RS04370 at 814.760 kb on + strand, within MMJJ_RS04370 at 814.770 kb on - strand, within MMJJ_RS04370 at 814.787 kb on + strand, within MMJJ_RS04370 at 814.791 kb on - strand, within MMJJ_RS04370 at 814.792 kb on + strand, within MMJJ_RS04370 at 814.812 kb on - strand, within MMJJ_RS04370 at 814.812 kb on - strand, within MMJJ_RS04370 at 814.813 kb on + strand, within MMJJ_RS04370 at 814.814 kb on - strand, within MMJJ_RS04370 at 814.814 kb on - strand, within MMJJ_RS04370 at 814.814 kb on - strand, within MMJJ_RS04370 at 814.814 kb on - strand, within MMJJ_RS04370 at 814.815 kb on + strand, within MMJJ_RS04370 at 814.816 kb on - strand, within MMJJ_RS04370 at 814.816 kb on - strand, within MMJJ_RS04370 at 814.816 kb on - strand, within MMJJ_RS04370 at 814.816 kb on - strand, within MMJJ_RS04370 at 814.817 kb on + strand, within MMJJ_RS04370 at 814.817 kb on + strand, within MMJJ_RS04370 at 814.817 kb on + strand, within MMJJ_RS04370 at 814.817 kb on + strand, within MMJJ_RS04370 at 814.817 kb on + strand, within MMJJ_RS04370
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 812,563 - -0.8 812,563 - -0.7 812,567 + -1.1 812,567 + -1.2 812,567 + -1.4 812,637 - MMJJ_RS04360 0.23 -1.5 812,657 + MMJJ_RS04360 0.30 -1.1 812,660 - MMJJ_RS04360 0.31 -1.0 812,660 - MMJJ_RS04360 0.31 -1.7 812,722 + MMJJ_RS04360 0.51 -1.1 812,768 - MMJJ_RS04360 0.66 -1.8 812,772 + MMJJ_RS04360 0.68 -1.3 812,773 - MMJJ_RS04360 0.68 -0.7 812,773 - MMJJ_RS04360 0.68 -1.1 812,773 - MMJJ_RS04360 0.68 -1.4 812,774 + MMJJ_RS04360 0.68 -1.1 812,774 + MMJJ_RS04360 0.68 -3.3 812,774 + MMJJ_RS04360 0.68 -0.7 812,836 - MMJJ_RS04360 0.89 -0.8 812,836 - MMJJ_RS04360 0.89 +0.4 812,837 + MMJJ_RS04360 0.89 +0.9 812,837 + MMJJ_RS04360 0.89 +0.4 812,837 + MMJJ_RS04360 0.89 +0.3 812,837 + MMJJ_RS04360 0.89 +0.3 812,837 + MMJJ_RS04360 0.89 -1.2 812,837 + MMJJ_RS04360 0.89 +0.0 812,837 + MMJJ_RS04360 0.89 -0.2 812,837 + MMJJ_RS04360 0.89 +0.5 812,837 + MMJJ_RS04360 0.89 +0.9 812,837 + MMJJ_RS04360 0.89 -1.3 812,837 + MMJJ_RS04360 0.89 +0.6 812,840 - +1.2 812,841 + +0.4 812,841 + +0.5 812,857 - +0.9 812,889 + +0.2 812,895 - +0.6 812,917 - -0.3 812,917 - -0.5 813,158 + +0.1 813,158 + -0.0 813,158 + -1.0 813,158 + -0.1 813,162 + +0.5 813,169 + -0.0 813,169 + +0.0 813,216 - +0.4 813,216 - +0.1 813,217 + -0.2 813,219 - -0.1 813,235 + +0.1 813,269 - +0.1 813,270 + +0.1 813,270 + -1.4 813,293 + -0.3 813,306 - +0.1 813,306 - +0.8 813,307 + -0.2 813,311 + +0.6 813,330 + -0.8 813,345 - +0.3 813,346 + +0.7 813,346 + -0.7 813,346 + +0.6 813,346 + -0.5 813,346 + -0.2 813,353 + -0.8 813,354 - -0.8 813,354 - +1.2 813,355 + -0.1 813,355 + +0.1 813,366 + -0.3 813,379 - -1.8 813,413 + +0.4 813,416 - -0.4 813,432 - +2.0 813,448 + +0.7 813,448 + +0.4 813,457 - -0.1 813,464 - -0.5 813,465 + +1.2 813,566 + -3.9 813,568 - -0.0 813,588 - MMJJ_RS04365 0.10 +0.8 813,591 - MMJJ_RS04365 0.11 +0.7 813,591 - MMJJ_RS04365 0.11 +0.2 813,592 + MMJJ_RS04365 0.11 +1.7 813,593 - MMJJ_RS04365 0.12 -2.3 813,593 - MMJJ_RS04365 0.12 -0.3 813,593 - MMJJ_RS04365 0.12 +0.2 813,598 + MMJJ_RS04365 0.14 +0.9 813,598 + MMJJ_RS04365 0.14 +0.1 813,633 - MMJJ_RS04365 0.26 +0.5 813,637 - MMJJ_RS04365 0.28 +0.4 813,637 - MMJJ_RS04365 0.28 +0.2 813,638 + MMJJ_RS04365 0.28 +0.9 813,641 + MMJJ_RS04365 0.29 -0.7 813,667 - MMJJ_RS04365 0.38 +1.2 813,690 - MMJJ_RS04365 0.47 -0.7 813,691 + MMJJ_RS04365 0.47 -0.6 813,701 + MMJJ_RS04365 0.51 -0.6 813,821 - +0.0 813,822 + -0.1 813,826 - -0.1 813,826 - -0.1 813,827 + +0.8 813,827 + +0.4 813,827 + +1.1 813,827 + -0.2 813,827 + +0.2 813,827 + +0.2 813,827 + -0.2 813,827 + -1.0 813,837 + -0.0 813,839 - +1.4 813,839 - -1.5 813,840 + -0.3 813,840 + -0.3 813,840 + +1.4 813,845 + +0.1 813,847 - +0.4 813,871 - -0.5 813,877 - +0.4 813,878 + +0.0 813,878 + -0.6 813,878 + +0.7 813,880 - -0.8 813,881 + -1.2 813,881 + -0.1 813,881 + -0.8 813,881 + +0.7 813,881 + -0.5 813,884 - +1.0 813,891 - +0.4 813,898 + -0.7 813,898 + +0.1 813,899 - +0.2 813,913 - +0.6 814,040 - -0.2 814,052 - -0.7 814,069 + +0.5 814,069 + -0.4 814,154 + -0.0 814,158 + -0.3 814,184 - +0.4 814,184 - -0.3 814,184 - +0.5 814,184 - -1.0 814,185 + +0.1 814,185 + +0.5 814,185 + +0.7 814,186 - +0.7 814,189 + -0.4 814,189 + -1.2 814,213 - -0.8 814,254 - +0.3 814,271 - +0.5 814,312 - -0.2 814,312 - -0.1 814,331 - +0.7 814,360 + -0.1 814,381 - -1.5 814,382 + +0.4 814,382 + +0.3 814,385 + +0.4 814,398 - -1.6 814,400 - +0.0 814,401 + +0.1 814,401 + -0.3 814,429 - +0.4 814,429 - -0.3 814,430 + -0.7 814,431 - -1.2 814,431 - +0.5 814,431 - -0.2 814,432 + -0.8 814,432 + +0.7 814,432 + -1.0 814,510 - MMJJ_RS04370 0.13 +0.8 814,517 - MMJJ_RS04370 0.14 -0.0 814,543 + MMJJ_RS04370 0.15 +0.1 814,543 + MMJJ_RS04370 0.15 +0.1 814,564 - MMJJ_RS04370 0.17 -0.0 814,564 - MMJJ_RS04370 0.17 -0.8 814,566 - MMJJ_RS04370 0.17 -0.3 814,567 + MMJJ_RS04370 0.17 +0.8 814,573 + MMJJ_RS04370 0.17 -0.8 814,573 + MMJJ_RS04370 0.17 +0.2 814,573 + MMJJ_RS04370 0.17 +0.1 814,573 + MMJJ_RS04370 0.17 -0.1 814,575 + MMJJ_RS04370 0.17 +0.1 814,596 - MMJJ_RS04370 0.19 +0.3 814,604 - MMJJ_RS04370 0.19 -0.0 814,638 - MMJJ_RS04370 0.21 +0.4 814,679 + MMJJ_RS04370 0.24 +0.3 814,693 - MMJJ_RS04370 0.24 +0.3 814,694 + MMJJ_RS04370 0.25 -1.2 814,694 + MMJJ_RS04370 0.25 +0.4 814,741 - MMJJ_RS04370 0.27 -1.8 814,750 - MMJJ_RS04370 0.28 +0.2 814,750 - MMJJ_RS04370 0.28 +0.3 814,751 + MMJJ_RS04370 0.28 +1.4 814,751 + MMJJ_RS04370 0.28 -1.0 814,751 + MMJJ_RS04370 0.28 -0.9 814,752 - MMJJ_RS04370 0.28 +0.3 814,752 - MMJJ_RS04370 0.28 +0.5 814,752 - MMJJ_RS04370 0.28 -0.5 814,753 + MMJJ_RS04370 0.28 +0.6 814,753 + MMJJ_RS04370 0.28 -2.8 814,759 - MMJJ_RS04370 0.28 -0.4 814,759 - MMJJ_RS04370 0.28 -0.1 814,760 + MMJJ_RS04370 0.29 -0.5 814,770 - MMJJ_RS04370 0.29 -0.4 814,787 + MMJJ_RS04370 0.30 +0.4 814,791 - MMJJ_RS04370 0.30 -0.3 814,792 + MMJJ_RS04370 0.30 -0.1 814,812 - MMJJ_RS04370 0.32 +0.1 814,812 - MMJJ_RS04370 0.32 -0.5 814,813 + MMJJ_RS04370 0.32 +0.3 814,814 - MMJJ_RS04370 0.32 -0.0 814,814 - MMJJ_RS04370 0.32 +0.3 814,814 - MMJJ_RS04370 0.32 +0.2 814,814 - MMJJ_RS04370 0.32 +0.2 814,815 + MMJJ_RS04370 0.32 -0.5 814,816 - MMJJ_RS04370 0.32 -0.2 814,816 - MMJJ_RS04370 0.32 +0.6 814,816 - MMJJ_RS04370 0.32 -1.1 814,816 - MMJJ_RS04370 0.32 -0.2 814,817 + MMJJ_RS04370 0.32 -3.9 814,817 + MMJJ_RS04370 0.32 +0.1 814,817 + MMJJ_RS04370 0.32 +0.1 814,817 + MMJJ_RS04370 0.32 -1.4 814,817 + MMJJ_RS04370 0.32 +0.3
Or see this region's nucleotide sequence