Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01310 and MMJJ_RS01315 are separated by 10 nucleotides MMJJ_RS01315 and MMJJ_RS01320 overlap by 1 nucleotides MMJJ_RS01320 and MMJJ_RS01325 are separated by 427 nucleotides
MMJJ_RS01310: MMJJ_RS01310 - phenylacetate--CoA ligase, at 250,283 to 251,581
_RS01310
MMJJ_RS01315: MMJJ_RS01315 - indolepyruvate oxidoreductase subunit beta, at 251,592 to 252,158
_RS01315
MMJJ_RS01320: MMJJ_RS01320 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 252,158 to 253,894
_RS01320
MMJJ_RS01325: MMJJ_RS01325 - magnesium/cobalt transporter CorA, at 254,322 to 255,380
_RS01325
Position (kb) 252
253
254 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 251.168 kb on + strand, within MMJJ_RS01310 at 251.184 kb on - strand, within MMJJ_RS01310 at 251.229 kb on + strand, within MMJJ_RS01310 at 251.243 kb on - strand, within MMJJ_RS01310 at 251.244 kb on + strand, within MMJJ_RS01310 at 251.282 kb on - strand, within MMJJ_RS01310 at 251.379 kb on + strand, within MMJJ_RS01310 at 251.396 kb on + strand, within MMJJ_RS01310 at 251.396 kb on + strand, within MMJJ_RS01310 at 251.452 kb on - strand at 251.453 kb on + strand at 251.453 kb on + strand at 251.500 kb on - strand at 251.506 kb on - strand at 251.564 kb on + strand at 251.564 kb on + strand at 251.665 kb on + strand, within MMJJ_RS01315 at 251.674 kb on + strand, within MMJJ_RS01315 at 251.742 kb on - strand, within MMJJ_RS01315 at 251.742 kb on - strand, within MMJJ_RS01315 at 251.769 kb on - strand, within MMJJ_RS01315 at 251.778 kb on - strand, within MMJJ_RS01315 at 251.787 kb on + strand, within MMJJ_RS01315 at 251.908 kb on + strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.921 kb on + strand, within MMJJ_RS01315 at 252.008 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.014 kb on + strand, within MMJJ_RS01315 at 252.014 kb on + strand, within MMJJ_RS01315 at 252.164 kb on - strand at 252.176 kb on - strand at 252.176 kb on - strand at 252.176 kb on - strand at 252.290 kb on - strand at 252.290 kb on - strand at 252.291 kb on + strand at 252.295 kb on - strand at 252.295 kb on - strand at 252.326 kb on + strand at 252.362 kb on - strand, within MMJJ_RS01320 at 252.363 kb on + strand, within MMJJ_RS01320 at 252.441 kb on + strand, within MMJJ_RS01320 at 252.470 kb on + strand, within MMJJ_RS01320 at 252.473 kb on - strand, within MMJJ_RS01320 at 252.474 kb on + strand, within MMJJ_RS01320 at 252.577 kb on - strand, within MMJJ_RS01320 at 252.577 kb on - strand, within MMJJ_RS01320 at 252.578 kb on + strand, within MMJJ_RS01320 at 252.763 kb on - strand, within MMJJ_RS01320 at 252.805 kb on + strand, within MMJJ_RS01320 at 252.814 kb on - strand, within MMJJ_RS01320 at 252.852 kb on - strand, within MMJJ_RS01320 at 252.853 kb on + strand, within MMJJ_RS01320 at 252.963 kb on + strand, within MMJJ_RS01320 at 253.011 kb on + strand, within MMJJ_RS01320 at 253.049 kb on + strand, within MMJJ_RS01320 at 253.067 kb on - strand, within MMJJ_RS01320 at 253.068 kb on + strand, within MMJJ_RS01320 at 253.068 kb on + strand, within MMJJ_RS01320 at 253.072 kb on + strand, within MMJJ_RS01320 at 253.182 kb on - strand, within MMJJ_RS01320 at 253.183 kb on + strand, within MMJJ_RS01320 at 253.222 kb on - strand, within MMJJ_RS01320 at 253.223 kb on + strand, within MMJJ_RS01320 at 253.240 kb on - strand, within MMJJ_RS01320 at 253.330 kb on + strand, within MMJJ_RS01320 at 253.345 kb on + strand, within MMJJ_RS01320 at 253.352 kb on - strand, within MMJJ_RS01320 at 253.352 kb on - strand, within MMJJ_RS01320 at 253.353 kb on + strand, within MMJJ_RS01320 at 253.353 kb on + strand, within MMJJ_RS01320 at 253.542 kb on - strand, within MMJJ_RS01320 at 253.543 kb on + strand, within MMJJ_RS01320 at 253.598 kb on - strand, within MMJJ_RS01320 at 253.599 kb on + strand, within MMJJ_RS01320 at 253.616 kb on + strand, within MMJJ_RS01320 at 253.693 kb on - strand, within MMJJ_RS01320 at 253.694 kb on + strand, within MMJJ_RS01320 at 253.784 kb on + strand at 253.898 kb on - strand at 253.899 kb on + strand at 253.899 kb on + strand at 253.914 kb on - strand at 253.915 kb on + strand at 253.936 kb on - strand at 253.951 kb on - strand at 253.972 kb on - strand at 253.974 kb on - strand at 253.974 kb on - strand at 253.974 kb on - strand at 253.975 kb on + strand at 253.975 kb on + strand at 253.975 kb on + strand at 254.009 kb on + strand at 254.022 kb on - strand at 254.026 kb on - strand at 254.027 kb on + strand at 254.027 kb on + strand at 254.032 kb on - strand at 254.033 kb on + strand at 254.033 kb on + strand at 254.033 kb on + strand at 254.050 kb on - strand at 254.061 kb on - strand at 254.074 kb on - strand at 254.075 kb on + strand at 254.084 kb on - strand at 254.112 kb on - strand at 254.117 kb on + strand at 254.117 kb on + strand at 254.133 kb on - strand at 254.134 kb on + strand at 254.155 kb on - strand at 254.231 kb on - strand at 254.231 kb on - strand at 254.232 kb on + strand at 254.232 kb on + strand at 254.233 kb on - strand at 254.233 kb on - strand at 254.233 kb on - strand at 254.234 kb on + strand at 254.234 kb on + strand at 254.234 kb on + strand at 254.234 kb on + strand at 254.234 kb on + strand at 254.234 kb on + strand at 254.235 kb on - strand at 254.236 kb on + strand at 254.236 kb on + strand at 254.238 kb on - strand at 254.239 kb on + strand at 254.239 kb on + strand at 254.242 kb on - strand at 254.367 kb on - strand at 254.367 kb on - strand at 254.384 kb on - strand at 254.385 kb on + strand at 254.426 kb on - strand at 254.479 kb on + strand, within MMJJ_RS01325 at 254.495 kb on - strand, within MMJJ_RS01325 at 254.545 kb on + strand, within MMJJ_RS01325 at 254.568 kb on + strand, within MMJJ_RS01325 at 254.616 kb on - strand, within MMJJ_RS01325 at 254.617 kb on + strand, within MMJJ_RS01325 at 254.630 kb on + strand, within MMJJ_RS01325 at 254.630 kb on + strand, within MMJJ_RS01325 at 254.630 kb on + strand, within MMJJ_RS01325 at 254.632 kb on + strand, within MMJJ_RS01325 at 254.635 kb on + strand, within MMJJ_RS01325 at 254.660 kb on + strand, within MMJJ_RS01325 at 254.737 kb on + strand, within MMJJ_RS01325 at 254.794 kb on - strand, within MMJJ_RS01325 at 254.795 kb on + strand, within MMJJ_RS01325 at 254.795 kb on + strand, within MMJJ_RS01325 at 254.795 kb on + strand, within MMJJ_RS01325 at 254.848 kb on + strand, within MMJJ_RS01325
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 251,168 + MMJJ_RS01310 0.68 -0.4 251,184 - MMJJ_RS01310 0.69 +0.1 251,229 + MMJJ_RS01310 0.73 -0.5 251,243 - MMJJ_RS01310 0.74 +0.3 251,244 + MMJJ_RS01310 0.74 +0.3 251,282 - MMJJ_RS01310 0.77 -0.6 251,379 + MMJJ_RS01310 0.84 -0.4 251,396 + MMJJ_RS01310 0.86 +0.0 251,396 + MMJJ_RS01310 0.86 -1.0 251,452 - -0.3 251,453 + -0.2 251,453 + -3.6 251,500 - -0.9 251,506 - -0.0 251,564 + -0.3 251,564 + +0.6 251,665 + MMJJ_RS01315 0.13 -4.6 251,674 + MMJJ_RS01315 0.14 -0.5 251,742 - MMJJ_RS01315 0.26 +0.1 251,742 - MMJJ_RS01315 0.26 +0.4 251,769 - MMJJ_RS01315 0.31 +0.2 251,778 - MMJJ_RS01315 0.33 -0.3 251,787 + MMJJ_RS01315 0.34 +0.1 251,908 + MMJJ_RS01315 0.56 -0.2 251,920 - MMJJ_RS01315 0.58 -0.9 251,920 - MMJJ_RS01315 0.58 +1.1 251,920 - MMJJ_RS01315 0.58 +0.9 251,921 + MMJJ_RS01315 0.58 -0.7 252,008 - MMJJ_RS01315 0.73 -0.1 252,013 - MMJJ_RS01315 0.74 -0.8 252,013 - MMJJ_RS01315 0.74 +0.8 252,013 - MMJJ_RS01315 0.74 -0.3 252,014 + MMJJ_RS01315 0.74 +3.3 252,014 + MMJJ_RS01315 0.74 +1.0 252,164 - +1.0 252,176 - -0.8 252,176 - -0.0 252,176 - +1.1 252,290 - +1.2 252,290 - -0.4 252,291 + -0.3 252,295 - +0.1 252,295 - +0.7 252,326 + +0.0 252,362 - MMJJ_RS01320 0.12 -0.3 252,363 + MMJJ_RS01320 0.12 -0.3 252,441 + MMJJ_RS01320 0.16 -0.4 252,470 + MMJJ_RS01320 0.18 +0.1 252,473 - MMJJ_RS01320 0.18 +1.7 252,474 + MMJJ_RS01320 0.18 -2.9 252,577 - MMJJ_RS01320 0.24 +0.5 252,577 - MMJJ_RS01320 0.24 +1.0 252,578 + MMJJ_RS01320 0.24 +1.1 252,763 - MMJJ_RS01320 0.35 +0.5 252,805 + MMJJ_RS01320 0.37 +0.2 252,814 - MMJJ_RS01320 0.38 -1.4 252,852 - MMJJ_RS01320 0.40 -0.3 252,853 + MMJJ_RS01320 0.40 +0.9 252,963 + MMJJ_RS01320 0.46 -0.8 253,011 + MMJJ_RS01320 0.49 +0.4 253,049 + MMJJ_RS01320 0.51 -0.7 253,067 - MMJJ_RS01320 0.52 +0.4 253,068 + MMJJ_RS01320 0.52 +0.5 253,068 + MMJJ_RS01320 0.52 +0.4 253,072 + MMJJ_RS01320 0.53 -0.7 253,182 - MMJJ_RS01320 0.59 -0.6 253,183 + MMJJ_RS01320 0.59 -0.2 253,222 - MMJJ_RS01320 0.61 +0.1 253,223 + MMJJ_RS01320 0.61 -0.9 253,240 - MMJJ_RS01320 0.62 -1.5 253,330 + MMJJ_RS01320 0.67 -0.3 253,345 + MMJJ_RS01320 0.68 +0.5 253,352 - MMJJ_RS01320 0.69 -0.0 253,352 - MMJJ_RS01320 0.69 -1.0 253,353 + MMJJ_RS01320 0.69 -0.2 253,353 + MMJJ_RS01320 0.69 +0.9 253,542 - MMJJ_RS01320 0.80 -0.7 253,543 + MMJJ_RS01320 0.80 +0.7 253,598 - MMJJ_RS01320 0.83 +0.3 253,599 + MMJJ_RS01320 0.83 +0.3 253,616 + MMJJ_RS01320 0.84 +0.6 253,693 - MMJJ_RS01320 0.88 +0.2 253,694 + MMJJ_RS01320 0.88 +0.2 253,784 + -0.5 253,898 - -0.3 253,899 + +1.4 253,899 + +0.6 253,914 - +0.2 253,915 + +0.1 253,936 - +0.2 253,951 - +0.5 253,972 - +1.3 253,974 - -0.1 253,974 - -2.2 253,974 - -0.6 253,975 + +0.3 253,975 + +0.5 253,975 + +0.4 254,009 + -0.3 254,022 - -0.7 254,026 - +0.1 254,027 + +1.0 254,027 + +0.6 254,032 - -1.1 254,033 + -0.9 254,033 + -0.3 254,033 + +0.9 254,050 - -1.3 254,061 - -0.3 254,074 - +0.3 254,075 + -0.9 254,084 - +0.7 254,112 - -0.9 254,117 + -0.3 254,117 + +0.3 254,133 - +1.1 254,134 + +0.3 254,155 - -0.7 254,231 - +0.8 254,231 - +0.6 254,232 + -1.1 254,232 + -0.5 254,233 - -1.0 254,233 - -1.3 254,233 - -0.4 254,234 + +0.4 254,234 + -0.2 254,234 + -0.3 254,234 + -0.2 254,234 + -0.2 254,234 + -0.1 254,235 - -0.9 254,236 + -0.1 254,236 + -0.0 254,238 - -0.7 254,239 + -0.2 254,239 + -0.0 254,242 - +0.6 254,367 - -0.1 254,367 - -0.1 254,384 - +0.7 254,385 + -0.3 254,426 - -0.7 254,479 + MMJJ_RS01325 0.15 +0.7 254,495 - MMJJ_RS01325 0.16 -0.1 254,545 + MMJJ_RS01325 0.21 +0.3 254,568 + MMJJ_RS01325 0.23 -1.0 254,616 - MMJJ_RS01325 0.28 +0.6 254,617 + MMJJ_RS01325 0.28 -1.0 254,630 + MMJJ_RS01325 0.29 -0.0 254,630 + MMJJ_RS01325 0.29 -0.5 254,630 + MMJJ_RS01325 0.29 +0.4 254,632 + MMJJ_RS01325 0.29 -0.6 254,635 + MMJJ_RS01325 0.30 -0.7 254,660 + MMJJ_RS01325 0.32 -0.3 254,737 + MMJJ_RS01325 0.39 +0.3 254,794 - MMJJ_RS01325 0.45 +0.1 254,795 + MMJJ_RS01325 0.45 +0.2 254,795 + MMJJ_RS01325 0.45 -0.5 254,795 + MMJJ_RS01325 0.45 -0.6 254,848 + MMJJ_RS01325 0.50 -0.7
Or see this region's nucleotide sequence