Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01020 and MMJJ_RS01030 are separated by 439 nucleotides MMJJ_RS01030 and MMJJ_RS01035 are separated by 154 nucleotides MMJJ_RS01035 and MMJJ_RS01040 are separated by 295 nucleotides
MMJJ_RS01020: MMJJ_RS01020 - serine protease, at 199,331 to 200,107
_RS01020
MMJJ_RS01030: MMJJ_RS01030 - hypothetical protein, at 200,547 to 202,052
_RS01030
MMJJ_RS01035: MMJJ_RS01035 - hypothetical protein, at 202,207 to 202,494
_RS01035
MMJJ_RS01040: MMJJ_RS01040 - DUF4013 domain-containing protein, at 202,790 to 203,407
_RS01040
Position (kb) 200
201
202
203 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 199.571 kb on - strand, within MMJJ_RS01020 at 199.575 kb on - strand, within MMJJ_RS01020 at 199.601 kb on + strand, within MMJJ_RS01020 at 199.606 kb on - strand, within MMJJ_RS01020 at 199.657 kb on - strand, within MMJJ_RS01020 at 199.679 kb on - strand, within MMJJ_RS01020 at 199.758 kb on + strand, within MMJJ_RS01020 at 199.817 kb on - strand, within MMJJ_RS01020 at 199.824 kb on + strand, within MMJJ_RS01020 at 199.826 kb on - strand, within MMJJ_RS01020 at 199.865 kb on - strand, within MMJJ_RS01020 at 199.866 kb on + strand, within MMJJ_RS01020 at 199.869 kb on - strand, within MMJJ_RS01020 at 199.869 kb on - strand, within MMJJ_RS01020 at 199.870 kb on + strand, within MMJJ_RS01020 at 199.870 kb on + strand, within MMJJ_RS01020 at 199.870 kb on + strand, within MMJJ_RS01020 at 199.870 kb on + strand at 199.870 kb on + strand, within MMJJ_RS01020 at 199.940 kb on - strand, within MMJJ_RS01020 at 199.941 kb on + strand, within MMJJ_RS01020 at 200.001 kb on + strand, within MMJJ_RS01020 at 200.003 kb on + strand, within MMJJ_RS01020 at 200.071 kb on - strand at 200.072 kb on + strand at 200.111 kb on - strand at 200.112 kb on + strand at 200.112 kb on + strand at 200.129 kb on - strand at 200.130 kb on + strand at 200.130 kb on + strand at 200.130 kb on + strand at 200.132 kb on - strand at 200.132 kb on - strand at 200.133 kb on + strand at 200.133 kb on + strand at 200.135 kb on - strand at 200.135 kb on - strand at 200.140 kb on + strand at 200.172 kb on - strand at 200.172 kb on - strand at 200.180 kb on - strand at 200.207 kb on - strand at 200.207 kb on - strand at 200.212 kb on - strand at 200.212 kb on - strand at 200.224 kb on + strand at 200.254 kb on - strand at 200.290 kb on + strand at 200.291 kb on - strand at 200.292 kb on + strand at 200.292 kb on + strand at 200.298 kb on + strand at 200.304 kb on + strand at 200.325 kb on - strand at 200.448 kb on - strand at 200.525 kb on - strand at 200.533 kb on - strand at 200.538 kb on - strand at 200.539 kb on + strand at 200.558 kb on - strand at 200.558 kb on - strand at 200.571 kb on + strand at 200.622 kb on + strand at 200.622 kb on + strand at 200.648 kb on - strand at 200.649 kb on + strand at 200.703 kb on + strand, within MMJJ_RS01030 at 200.705 kb on - strand, within MMJJ_RS01030 at 200.705 kb on - strand, within MMJJ_RS01030 at 200.706 kb on + strand, within MMJJ_RS01030 at 200.709 kb on - strand, within MMJJ_RS01030 at 200.775 kb on + strand, within MMJJ_RS01030 at 200.778 kb on - strand, within MMJJ_RS01030 at 200.873 kb on + strand, within MMJJ_RS01030 at 200.880 kb on - strand, within MMJJ_RS01030 at 200.925 kb on + strand, within MMJJ_RS01030 at 200.925 kb on + strand, within MMJJ_RS01030 at 200.927 kb on + strand, within MMJJ_RS01030 at 200.927 kb on + strand, within MMJJ_RS01030 at 200.927 kb on + strand, within MMJJ_RS01030 at 200.927 kb on + strand, within MMJJ_RS01030 at 200.942 kb on - strand, within MMJJ_RS01030 at 200.957 kb on - strand, within MMJJ_RS01030 at 200.958 kb on + strand, within MMJJ_RS01030 at 200.987 kb on - strand, within MMJJ_RS01030 at 200.987 kb on - strand, within MMJJ_RS01030 at 200.987 kb on - strand, within MMJJ_RS01030 at 200.988 kb on + strand, within MMJJ_RS01030 at 200.988 kb on + strand, within MMJJ_RS01030 at 200.988 kb on + strand, within MMJJ_RS01030 at 200.988 kb on + strand, within MMJJ_RS01030 at 200.988 kb on + strand, within MMJJ_RS01030 at 201.000 kb on - strand, within MMJJ_RS01030 at 201.001 kb on + strand, within MMJJ_RS01030 at 201.005 kb on + strand, within MMJJ_RS01030 at 201.088 kb on - strand, within MMJJ_RS01030 at 201.127 kb on + strand, within MMJJ_RS01030 at 201.127 kb on + strand, within MMJJ_RS01030 at 201.146 kb on - strand, within MMJJ_RS01030 at 201.190 kb on + strand, within MMJJ_RS01030 at 201.190 kb on + strand, within MMJJ_RS01030 at 201.197 kb on - strand, within MMJJ_RS01030 at 201.198 kb on + strand, within MMJJ_RS01030 at 201.216 kb on - strand, within MMJJ_RS01030 at 201.221 kb on - strand, within MMJJ_RS01030 at 201.227 kb on - strand, within MMJJ_RS01030 at 201.230 kb on + strand, within MMJJ_RS01030 at 201.263 kb on + strand, within MMJJ_RS01030 at 201.356 kb on - strand, within MMJJ_RS01030 at 201.356 kb on - strand, within MMJJ_RS01030 at 201.356 kb on - strand, within MMJJ_RS01030 at 201.356 kb on - strand, within MMJJ_RS01030 at 201.357 kb on + strand, within MMJJ_RS01030 at 201.357 kb on + strand, within MMJJ_RS01030 at 201.360 kb on - strand, within MMJJ_RS01030 at 201.360 kb on - strand, within MMJJ_RS01030 at 201.360 kb on - strand, within MMJJ_RS01030 at 201.361 kb on + strand, within MMJJ_RS01030 at 201.425 kb on - strand, within MMJJ_RS01030 at 201.505 kb on - strand, within MMJJ_RS01030 at 201.506 kb on + strand, within MMJJ_RS01030 at 201.561 kb on - strand, within MMJJ_RS01030 at 201.567 kb on - strand, within MMJJ_RS01030 at 201.568 kb on + strand, within MMJJ_RS01030 at 201.568 kb on + strand, within MMJJ_RS01030 at 201.568 kb on + strand, within MMJJ_RS01030 at 201.576 kb on - strand, within MMJJ_RS01030 at 201.626 kb on + strand, within MMJJ_RS01030 at 201.657 kb on - strand, within MMJJ_RS01030 at 201.730 kb on - strand, within MMJJ_RS01030 at 201.730 kb on - strand, within MMJJ_RS01030 at 201.731 kb on + strand, within MMJJ_RS01030 at 201.731 kb on + strand, within MMJJ_RS01030 at 201.735 kb on - strand, within MMJJ_RS01030 at 201.740 kb on + strand, within MMJJ_RS01030 at 201.740 kb on + strand, within MMJJ_RS01030 at 201.746 kb on - strand, within MMJJ_RS01030 at 201.748 kb on - strand, within MMJJ_RS01030 at 201.758 kb on + strand, within MMJJ_RS01030 at 201.800 kb on + strand, within MMJJ_RS01030 at 201.809 kb on - strand, within MMJJ_RS01030 at 201.809 kb on - strand, within MMJJ_RS01030 at 201.810 kb on + strand, within MMJJ_RS01030 at 201.810 kb on + strand, within MMJJ_RS01030 at 201.810 kb on + strand, within MMJJ_RS01030 at 201.815 kb on - strand, within MMJJ_RS01030 at 201.816 kb on + strand at 201.822 kb on - strand, within MMJJ_RS01030 at 201.882 kb on - strand, within MMJJ_RS01030 at 201.919 kb on - strand at 201.933 kb on + strand at 201.985 kb on + strand at 202.034 kb on - strand at 202.064 kb on - strand at 202.064 kb on - strand at 202.065 kb on + strand at 202.066 kb on - strand at 202.066 kb on - strand at 202.066 kb on - strand at 202.067 kb on + strand at 202.067 kb on - strand at 202.075 kb on - strand at 202.075 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.078 kb on + strand at 202.078 kb on + strand at 202.080 kb on - strand at 202.081 kb on + strand at 202.081 kb on + strand at 202.082 kb on - strand at 202.082 kb on - strand at 202.082 kb on - strand at 202.083 kb on + strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.086 kb on + strand at 202.086 kb on + strand at 202.086 kb on + strand at 202.086 kb on + strand at 202.128 kb on - strand at 202.187 kb on - strand at 202.187 kb on - strand at 202.188 kb on + strand at 202.232 kb on - strand at 202.233 kb on + strand at 202.252 kb on + strand, within MMJJ_RS01035 at 202.252 kb on + strand, within MMJJ_RS01035 at 202.252 kb on + strand, within MMJJ_RS01035 at 202.328 kb on - strand, within MMJJ_RS01035 at 202.399 kb on + strand, within MMJJ_RS01035 at 202.407 kb on - strand, within MMJJ_RS01035 at 202.407 kb on - strand, within MMJJ_RS01035 at 202.407 kb on - strand, within MMJJ_RS01035 at 202.409 kb on - strand, within MMJJ_RS01035 at 202.409 kb on - strand, within MMJJ_RS01035 at 202.410 kb on + strand, within MMJJ_RS01035 at 202.410 kb on + strand, within MMJJ_RS01035 at 202.437 kb on - strand, within MMJJ_RS01035 at 202.492 kb on - strand at 202.529 kb on - strand at 202.530 kb on + strand at 202.558 kb on + strand at 202.561 kb on - strand at 202.561 kb on - strand at 202.561 kb on - strand at 202.583 kb on + strand at 202.583 kb on + strand at 202.583 kb on + strand at 202.621 kb on - strand at 202.621 kb on - strand at 202.621 kb on - strand at 202.621 kb on - strand at 202.621 kb on - strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.622 kb on + strand at 202.623 kb on - strand at 202.624 kb on + strand at 202.624 kb on + strand at 202.752 kb on - strand at 202.766 kb on - strand at 202.766 kb on - strand at 202.766 kb on - strand at 202.776 kb on + strand at 202.776 kb on + strand at 202.803 kb on + strand at 202.831 kb on + strand at 202.831 kb on + strand at 202.833 kb on - strand at 202.833 kb on - strand at 202.833 kb on - strand at 202.833 kb on - strand at 202.833 kb on - strand at 202.834 kb on + strand at 202.835 kb on - strand at 202.835 kb on - strand at 202.836 kb on + strand at 202.877 kb on - strand, within MMJJ_RS01040 at 202.890 kb on + strand, within MMJJ_RS01040 at 202.893 kb on - strand, within MMJJ_RS01040 at 203.015 kb on + strand, within MMJJ_RS01040 at 203.020 kb on + strand, within MMJJ_RS01040 at 203.021 kb on - strand, within MMJJ_RS01040
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 199,571 - MMJJ_RS01020 0.31 +0.2 199,575 - MMJJ_RS01020 0.31 -0.1 199,601 + MMJJ_RS01020 0.35 +0.4 199,606 - MMJJ_RS01020 0.35 +0.1 199,657 - MMJJ_RS01020 0.42 -0.6 199,679 - MMJJ_RS01020 0.45 +0.2 199,758 + MMJJ_RS01020 0.55 -2.1 199,817 - MMJJ_RS01020 0.63 -0.2 199,824 + MMJJ_RS01020 0.63 -0.3 199,826 - MMJJ_RS01020 0.64 -0.2 199,865 - MMJJ_RS01020 0.69 +0.4 199,866 + MMJJ_RS01020 0.69 -0.7 199,869 - MMJJ_RS01020 0.69 +0.6 199,869 - MMJJ_RS01020 0.69 -0.7 199,870 + MMJJ_RS01020 0.69 -1.0 199,870 + MMJJ_RS01020 0.69 +0.4 199,870 + MMJJ_RS01020 0.69 -2.2 199,870 + -0.0 199,870 + MMJJ_RS01020 0.69 +0.1 199,940 - MMJJ_RS01020 0.78 -0.3 199,941 + MMJJ_RS01020 0.79 -0.5 200,001 + MMJJ_RS01020 0.86 +0.6 200,003 + MMJJ_RS01020 0.86 -0.4 200,071 - -0.1 200,072 + -0.0 200,111 - -0.5 200,112 + -0.9 200,112 + -0.2 200,129 - +0.2 200,130 + +0.2 200,130 + +0.8 200,130 + -1.1 200,132 - -1.5 200,132 - -0.4 200,133 + +0.1 200,133 + -0.0 200,135 - +0.4 200,135 - -0.3 200,140 + +0.3 200,172 - +0.1 200,172 - +0.4 200,180 - +0.8 200,207 - -0.1 200,207 - -0.0 200,212 - +0.6 200,212 - -2.5 200,224 + -0.2 200,254 - -1.0 200,290 + +1.3 200,291 - +0.7 200,292 + +0.5 200,292 + -0.0 200,298 + +0.6 200,304 + -0.1 200,325 - +0.1 200,448 - +0.2 200,525 - +1.3 200,533 - +0.6 200,538 - +0.1 200,539 + -0.6 200,558 - -1.2 200,558 - +0.2 200,571 + -0.1 200,622 + -0.9 200,622 + -0.6 200,648 - -0.1 200,649 + +0.0 200,703 + MMJJ_RS01030 0.10 -0.4 200,705 - MMJJ_RS01030 0.10 -0.3 200,705 - MMJJ_RS01030 0.10 +0.6 200,706 + MMJJ_RS01030 0.11 +0.3 200,709 - MMJJ_RS01030 0.11 +0.3 200,775 + MMJJ_RS01030 0.15 -0.7 200,778 - MMJJ_RS01030 0.15 +0.2 200,873 + MMJJ_RS01030 0.22 +0.5 200,880 - MMJJ_RS01030 0.22 +0.3 200,925 + MMJJ_RS01030 0.25 +0.3 200,925 + MMJJ_RS01030 0.25 -0.6 200,927 + MMJJ_RS01030 0.25 +0.4 200,927 + MMJJ_RS01030 0.25 -0.5 200,927 + MMJJ_RS01030 0.25 +0.0 200,927 + MMJJ_RS01030 0.25 +0.6 200,942 - MMJJ_RS01030 0.26 +0.3 200,957 - MMJJ_RS01030 0.27 +0.6 200,958 + MMJJ_RS01030 0.27 -0.3 200,987 - MMJJ_RS01030 0.29 +0.4 200,987 - MMJJ_RS01030 0.29 +0.4 200,987 - MMJJ_RS01030 0.29 +0.1 200,988 + MMJJ_RS01030 0.29 -0.6 200,988 + MMJJ_RS01030 0.29 +0.9 200,988 + MMJJ_RS01030 0.29 -0.1 200,988 + MMJJ_RS01030 0.29 +0.4 200,988 + MMJJ_RS01030 0.29 +1.6 201,000 - MMJJ_RS01030 0.30 +0.4 201,001 + MMJJ_RS01030 0.30 -0.1 201,005 + MMJJ_RS01030 0.30 +0.1 201,088 - MMJJ_RS01030 0.36 +0.1 201,127 + MMJJ_RS01030 0.39 -0.2 201,127 + MMJJ_RS01030 0.39 -1.5 201,146 - MMJJ_RS01030 0.40 -0.4 201,190 + MMJJ_RS01030 0.43 +0.1 201,190 + MMJJ_RS01030 0.43 +0.3 201,197 - MMJJ_RS01030 0.43 -0.0 201,198 + MMJJ_RS01030 0.43 +1.3 201,216 - MMJJ_RS01030 0.44 +0.0 201,221 - MMJJ_RS01030 0.45 +0.5 201,227 - MMJJ_RS01030 0.45 +0.5 201,230 + MMJJ_RS01030 0.45 +0.2 201,263 + MMJJ_RS01030 0.48 +0.9 201,356 - MMJJ_RS01030 0.54 +0.7 201,356 - MMJJ_RS01030 0.54 -0.5 201,356 - MMJJ_RS01030 0.54 +0.2 201,356 - MMJJ_RS01030 0.54 +0.1 201,357 + MMJJ_RS01030 0.54 -0.0 201,357 + MMJJ_RS01030 0.54 +0.2 201,360 - MMJJ_RS01030 0.54 +0.7 201,360 - MMJJ_RS01030 0.54 -0.0 201,360 - MMJJ_RS01030 0.54 -1.0 201,361 + MMJJ_RS01030 0.54 -0.4 201,425 - MMJJ_RS01030 0.58 +1.2 201,505 - MMJJ_RS01030 0.64 +1.7 201,506 + MMJJ_RS01030 0.64 +1.7 201,561 - MMJJ_RS01030 0.67 -0.3 201,567 - MMJJ_RS01030 0.68 +0.3 201,568 + MMJJ_RS01030 0.68 -0.4 201,568 + MMJJ_RS01030 0.68 +0.4 201,568 + MMJJ_RS01030 0.68 -0.3 201,576 - MMJJ_RS01030 0.68 +0.0 201,626 + MMJJ_RS01030 0.72 +0.9 201,657 - MMJJ_RS01030 0.74 -0.9 201,730 - MMJJ_RS01030 0.79 +0.1 201,730 - MMJJ_RS01030 0.79 -0.7 201,731 + MMJJ_RS01030 0.79 -0.2 201,731 + MMJJ_RS01030 0.79 +0.6 201,735 - MMJJ_RS01030 0.79 +0.3 201,740 + MMJJ_RS01030 0.79 +0.6 201,740 + MMJJ_RS01030 0.79 -1.4 201,746 - MMJJ_RS01030 0.80 -0.6 201,748 - MMJJ_RS01030 0.80 -4.0 201,758 + MMJJ_RS01030 0.80 -0.1 201,800 + MMJJ_RS01030 0.83 -0.1 201,809 - MMJJ_RS01030 0.84 +0.1 201,809 - MMJJ_RS01030 0.84 +0.6 201,810 + MMJJ_RS01030 0.84 -0.1 201,810 + MMJJ_RS01030 0.84 +0.8 201,810 + MMJJ_RS01030 0.84 +0.1 201,815 - MMJJ_RS01030 0.84 -0.6 201,816 + +0.3 201,822 - MMJJ_RS01030 0.85 +0.3 201,882 - MMJJ_RS01030 0.89 +0.1 201,919 - +0.5 201,933 + -0.0 201,985 + -3.2 202,034 - -0.2 202,064 - -0.4 202,064 - +0.6 202,065 + -0.8 202,066 - +0.0 202,066 - +0.7 202,066 - -0.1 202,067 + +0.1 202,067 - -1.5 202,075 - -0.1 202,075 - +0.4 202,077 - -0.6 202,077 - -0.4 202,077 - -0.4 202,077 - -0.1 202,078 + +0.6 202,078 + +0.1 202,080 - +0.5 202,081 + +0.0 202,081 + +0.2 202,082 - -0.2 202,082 - -1.1 202,082 - +0.1 202,083 + +0.7 202,085 - +0.2 202,085 - -0.2 202,085 - -0.6 202,085 - -0.1 202,086 + -0.1 202,086 + +0.3 202,086 + +0.8 202,086 + +0.2 202,128 - +0.2 202,187 - +0.1 202,187 - -1.4 202,188 + +0.1 202,232 - -3.7 202,233 + +0.4 202,252 + MMJJ_RS01035 0.16 -1.1 202,252 + MMJJ_RS01035 0.16 -0.1 202,252 + MMJJ_RS01035 0.16 +0.7 202,328 - MMJJ_RS01035 0.42 -0.8 202,399 + MMJJ_RS01035 0.67 -0.2 202,407 - MMJJ_RS01035 0.69 +0.5 202,407 - MMJJ_RS01035 0.69 -0.1 202,407 - MMJJ_RS01035 0.69 +0.6 202,409 - MMJJ_RS01035 0.70 -1.3 202,409 - MMJJ_RS01035 0.70 +0.2 202,410 + MMJJ_RS01035 0.70 -0.9 202,410 + MMJJ_RS01035 0.70 -0.7 202,437 - MMJJ_RS01035 0.80 -1.6 202,492 - -1.0 202,529 - +0.3 202,530 + +0.2 202,558 + +0.3 202,561 - -0.2 202,561 - +0.5 202,561 - +1.1 202,583 + -0.4 202,583 + -0.1 202,583 + -0.1 202,621 - -0.4 202,621 - -0.1 202,621 - +0.4 202,621 - +0.6 202,621 - +0.1 202,622 + -0.4 202,622 + +0.2 202,622 + -0.6 202,622 + -0.4 202,622 + +0.9 202,622 + -0.1 202,622 + +2.3 202,622 + -0.4 202,623 - +1.0 202,624 + -0.8 202,624 + +0.1 202,752 - -0.7 202,766 - -0.1 202,766 - -0.0 202,766 - -0.1 202,776 + -0.7 202,776 + +0.8 202,803 + -0.7 202,831 + -0.1 202,831 + -0.1 202,833 - -0.5 202,833 - -0.5 202,833 - +0.2 202,833 - -0.3 202,833 - -0.2 202,834 + -0.8 202,835 - -0.5 202,835 - -0.5 202,836 + +0.3 202,877 - MMJJ_RS01040 0.14 -0.5 202,890 + MMJJ_RS01040 0.16 +0.3 202,893 - MMJJ_RS01040 0.17 -0.6 203,015 + MMJJ_RS01040 0.36 +0.5 203,020 + MMJJ_RS01040 0.37 -0.4 203,021 - MMJJ_RS01040 0.37 -0.3
Or see this region's nucleotide sequence