Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15215 and BBR_RS15220 are separated by 297 nucleotides BBR_RS15220 and BBR_RS15225 are separated by 250 nucleotides BBR_RS15225 and BBR_RS15230 are separated by 146 nucleotides
BBR_RS15215: BBR_RS15215 - MazG-like protein, at 1,182,188 to 1,183,069
_RS15215
BBR_RS15220: BBR_RS15220 - trimeric intracellular cation channel family protein, at 1,183,367 to 1,184,137
_RS15220
BBR_RS15225: BBR_RS15225 - 30S ribosomal protein S20, at 1,184,388 to 1,184,648
_RS15225
BBR_RS15230: BBR_RS15230 - elongation factor 4, at 1,184,795 to 1,186,675
_RS15230
Position (kb)
1183
1184
1185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1182.428 kb on + strand, within BBR_RS15215 at 1182.512 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.536 kb on - strand, within BBR_RS15215 at 1182.728 kb on - strand, within BBR_RS15215 at 1182.744 kb on + strand, within BBR_RS15215 at 1182.750 kb on - strand, within BBR_RS15215 at 1182.794 kb on - strand, within BBR_RS15215 at 1182.830 kb on - strand, within BBR_RS15215 at 1182.858 kb on + strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.927 kb on - strand, within BBR_RS15215 at 1182.956 kb on + strand, within BBR_RS15215 at 1183.077 kb on - strand at 1183.145 kb on + strand at 1183.242 kb on - strand at 1183.258 kb on + strand at 1183.265 kb on - strand at 1183.265 kb on - strand at 1183.265 kb on - strand at 1183.266 kb on - strand at 1183.285 kb on + strand at 1183.347 kb on + strand at 1183.428 kb on + strand at 1183.466 kb on + strand, within BBR_RS15220 at 1183.466 kb on + strand, within BBR_RS15220 at 1183.474 kb on - strand, within BBR_RS15220 at 1183.482 kb on + strand, within BBR_RS15220 at 1183.482 kb on + strand, within BBR_RS15220 at 1183.490 kb on - strand, within BBR_RS15220 at 1183.520 kb on + strand, within BBR_RS15220 at 1183.535 kb on + strand, within BBR_RS15220 at 1183.541 kb on - strand, within BBR_RS15220 at 1183.543 kb on - strand, within BBR_RS15220 at 1183.555 kb on - strand, within BBR_RS15220 at 1183.578 kb on + strand, within BBR_RS15220 at 1183.579 kb on - strand, within BBR_RS15220 at 1183.586 kb on - strand, within BBR_RS15220 at 1183.650 kb on - strand, within BBR_RS15220 at 1183.671 kb on - strand, within BBR_RS15220 at 1183.834 kb on + strand, within BBR_RS15220 at 1183.839 kb on + strand, within BBR_RS15220 at 1183.842 kb on + strand, within BBR_RS15220 at 1183.842 kb on - strand, within BBR_RS15220 at 1183.870 kb on + strand, within BBR_RS15220 at 1183.890 kb on + strand, within BBR_RS15220 at 1183.896 kb on + strand, within BBR_RS15220 at 1183.972 kb on + strand, within BBR_RS15220 at 1183.991 kb on - strand, within BBR_RS15220 at 1184.077 kb on + strand at 1184.077 kb on + strand at 1184.085 kb on - strand at 1184.091 kb on + strand at 1184.113 kb on - strand at 1184.115 kb on + strand at 1184.123 kb on - strand at 1184.123 kb on - strand at 1184.123 kb on - strand at 1184.224 kb on - strand at 1184.379 kb on - strand at 1184.744 kb on + strand at 1184.744 kb on + strand at 1184.749 kb on + strand at 1184.749 kb on + strand at 1184.801 kb on + strand at 1184.888 kb on - strand at 1184.888 kb on - strand at 1184.901 kb on + strand at 1184.909 kb on - strand at 1185.042 kb on + strand, within BBR_RS15230 at 1185.066 kb on + strand, within BBR_RS15230
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 1,182,428 + BBR_RS15215 0.27 +0.3 1,182,512 + BBR_RS15215 0.37 -0.1 1,182,528 + BBR_RS15215 0.39 +0.2 1,182,528 + BBR_RS15215 0.39 +0.4 1,182,536 - BBR_RS15215 0.39 -0.2 1,182,728 - BBR_RS15215 0.61 -0.9 1,182,744 + BBR_RS15215 0.63 +0.7 1,182,750 - BBR_RS15215 0.64 -0.2 1,182,794 - BBR_RS15215 0.69 -0.7 1,182,830 - BBR_RS15215 0.73 -1.0 1,182,858 + BBR_RS15215 0.76 +0.1 1,182,866 - BBR_RS15215 0.77 +0.4 1,182,866 - BBR_RS15215 0.77 +0.8 1,182,927 - BBR_RS15215 0.84 -0.2 1,182,956 + BBR_RS15215 0.87 +1.1 1,183,077 - -0.2 1,183,145 + -1.2 1,183,242 - -0.4 1,183,258 + -1.1 1,183,265 - -0.1 1,183,265 - +0.6 1,183,265 - +0.5 1,183,266 - -0.0 1,183,285 + +1.1 1,183,347 + +0.3 1,183,428 + +0.1 1,183,466 + BBR_RS15220 0.13 +0.3 1,183,466 + BBR_RS15220 0.13 +1.5 1,183,474 - BBR_RS15220 0.14 -0.6 1,183,482 + BBR_RS15220 0.15 +2.5 1,183,482 + BBR_RS15220 0.15 -0.9 1,183,490 - BBR_RS15220 0.16 -0.6 1,183,520 + BBR_RS15220 0.20 -0.7 1,183,535 + BBR_RS15220 0.22 -0.6 1,183,541 - BBR_RS15220 0.23 -0.6 1,183,543 - BBR_RS15220 0.23 -0.9 1,183,555 - BBR_RS15220 0.24 +0.3 1,183,578 + BBR_RS15220 0.27 +1.2 1,183,579 - BBR_RS15220 0.27 -1.1 1,183,586 - BBR_RS15220 0.28 -1.1 1,183,650 - BBR_RS15220 0.37 +0.8 1,183,671 - BBR_RS15220 0.39 -0.5 1,183,834 + BBR_RS15220 0.61 +0.6 1,183,839 + BBR_RS15220 0.61 -0.3 1,183,842 + BBR_RS15220 0.62 +0.1 1,183,842 - BBR_RS15220 0.62 -1.6 1,183,870 + BBR_RS15220 0.65 -0.6 1,183,890 + BBR_RS15220 0.68 +0.8 1,183,896 + BBR_RS15220 0.69 +0.5 1,183,972 + BBR_RS15220 0.78 -0.5 1,183,991 - BBR_RS15220 0.81 -0.7 1,184,077 + -0.3 1,184,077 + -1.1 1,184,085 - -1.7 1,184,091 + +0.2 1,184,113 - -1.8 1,184,115 + -3.0 1,184,123 - -1.7 1,184,123 - -0.1 1,184,123 - -1.7 1,184,224 - -2.4 1,184,379 - -0.2 1,184,744 + -2.3 1,184,744 + +0.3 1,184,749 + +0.3 1,184,749 + +0.8 1,184,801 + -1.4 1,184,888 - -1.1 1,184,888 - -0.9 1,184,901 + -1.8 1,184,909 - -0.6 1,185,042 + BBR_RS15230 0.13 -1.5 1,185,066 + BBR_RS15230 0.14 -1.4
Or see this region's nucleotide sequence