Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13875 and BBR_RS13880 are separated by 27 nucleotides BBR_RS13880 and BBR_RS13885 are separated by 96 nucleotides BBR_RS13885 and BBR_RS13890 overlap by 4 nucleotides
BBR_RS13875: BBR_RS13875 - phenylalanine--tRNA ligase subunit beta, at 891,515 to 894,124
_RS13875
BBR_RS13880: BBR_RS13880 - hypothetical protein, at 894,152 to 894,808
_RS13880
BBR_RS13885: BBR_RS13885 - N-acetyl-gamma-glutamyl-phosphate reductase, at 894,905 to 895,999
_RS13885
BBR_RS13890: BBR_RS13890 - bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ, at 895,996 to 897,171
_RS13890
Position (kb)
894
895
896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 894.126 kb on - strand at 894.128 kb on + strand at 894.128 kb on - strand at 894.128 kb on - strand at 894.164 kb on + strand at 894.174 kb on - strand at 894.174 kb on - strand at 894.174 kb on - strand at 894.199 kb on - strand at 894.234 kb on - strand, within BBR_RS13880 at 894.245 kb on - strand, within BBR_RS13880 at 894.245 kb on - strand, within BBR_RS13880 at 894.296 kb on + strand, within BBR_RS13880 at 894.306 kb on - strand, within BBR_RS13880 at 894.345 kb on - strand, within BBR_RS13880 at 894.384 kb on - strand, within BBR_RS13880 at 894.389 kb on + strand, within BBR_RS13880 at 894.397 kb on - strand, within BBR_RS13880 at 894.413 kb on + strand, within BBR_RS13880 at 894.448 kb on + strand, within BBR_RS13880 at 894.483 kb on - strand, within BBR_RS13880 at 894.528 kb on - strand, within BBR_RS13880 at 894.557 kb on - strand, within BBR_RS13880 at 894.557 kb on - strand, within BBR_RS13880 at 894.568 kb on + strand, within BBR_RS13880 at 894.568 kb on + strand, within BBR_RS13880 at 894.604 kb on - strand, within BBR_RS13880 at 894.653 kb on - strand, within BBR_RS13880 at 894.673 kb on + strand, within BBR_RS13880 at 894.682 kb on + strand, within BBR_RS13880 at 894.682 kb on + strand, within BBR_RS13880 at 894.692 kb on - strand, within BBR_RS13880 at 894.698 kb on - strand, within BBR_RS13880 at 894.777 kb on - strand at 894.798 kb on + strand at 894.803 kb on + strand at 894.803 kb on + strand at 894.808 kb on + strand at 894.811 kb on - strand at 894.811 kb on - strand at 894.833 kb on + strand at 894.909 kb on - strand at 894.949 kb on - strand at 894.999 kb on + strand at 895.087 kb on + strand, within BBR_RS13885 at 895.087 kb on + strand, within BBR_RS13885 at 895.092 kb on + strand, within BBR_RS13885 at 895.095 kb on - strand, within BBR_RS13885 at 895.095 kb on - strand, within BBR_RS13885 at 895.156 kb on + strand, within BBR_RS13885 at 895.193 kb on + strand, within BBR_RS13885 at 895.281 kb on + strand, within BBR_RS13885 at 895.283 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.344 kb on - strand, within BBR_RS13885 at 895.371 kb on + strand, within BBR_RS13885 at 895.383 kb on + strand, within BBR_RS13885 at 895.386 kb on + strand, within BBR_RS13885 at 895.415 kb on + strand, within BBR_RS13885 at 895.421 kb on + strand, within BBR_RS13885 at 895.429 kb on - strand, within BBR_RS13885 at 895.465 kb on - strand, within BBR_RS13885 at 895.468 kb on + strand, within BBR_RS13885 at 895.542 kb on - strand, within BBR_RS13885 at 895.542 kb on - strand, within BBR_RS13885 at 895.600 kb on + strand, within BBR_RS13885 at 895.627 kb on + strand, within BBR_RS13885 at 895.663 kb on + strand, within BBR_RS13885 at 895.663 kb on + strand, within BBR_RS13885 at 895.681 kb on - strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.716 kb on - strand, within BBR_RS13885 at 895.716 kb on - strand, within BBR_RS13885 at 895.723 kb on + strand, within BBR_RS13885 at 895.763 kb on + strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.794 kb on - strand, within BBR_RS13885 at 895.806 kb on + strand, within BBR_RS13885 at 895.848 kb on - strand, within BBR_RS13885 at 895.894 kb on + strand at 895.914 kb on - strand at 895.949 kb on - strand at 895.973 kb on - strand at 895.983 kb on - strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.988 kb on + strand at 895.988 kb on + strand at 895.989 kb on - strand at 895.989 kb on - strand at 896.062 kb on + strand at 896.094 kb on - strand at 896.112 kb on + strand at 896.141 kb on + strand, within BBR_RS13890 at 896.141 kb on + strand, within BBR_RS13890 at 896.144 kb on - strand, within BBR_RS13890 at 896.149 kb on + strand, within BBR_RS13890 at 896.149 kb on + strand, within BBR_RS13890 at 896.152 kb on + strand, within BBR_RS13890 at 896.163 kb on + strand, within BBR_RS13890 at 896.170 kb on + strand, within BBR_RS13890 at 896.223 kb on + strand, within BBR_RS13890 at 896.231 kb on + strand, within BBR_RS13890 at 896.236 kb on - strand, within BBR_RS13890 at 896.251 kb on - strand, within BBR_RS13890 at 896.265 kb on + strand, within BBR_RS13890 at 896.265 kb on + strand, within BBR_RS13890 at 896.304 kb on + strand, within BBR_RS13890 at 896.306 kb on + strand, within BBR_RS13890 at 896.321 kb on + strand, within BBR_RS13890 at 896.326 kb on + strand, within BBR_RS13890 at 896.409 kb on + strand, within BBR_RS13890 at 896.414 kb on + strand, within BBR_RS13890 at 896.425 kb on + strand, within BBR_RS13890 at 896.462 kb on + strand, within BBR_RS13890 at 896.462 kb on + strand, within BBR_RS13890 at 896.464 kb on + strand, within BBR_RS13890 at 896.472 kb on + strand, within BBR_RS13890 at 896.506 kb on + strand, within BBR_RS13890 at 896.513 kb on - strand, within BBR_RS13890 at 896.540 kb on + strand, within BBR_RS13890 at 896.550 kb on - strand, within BBR_RS13890 at 896.569 kb on + strand, within BBR_RS13890 at 896.574 kb on + strand, within BBR_RS13890 at 896.574 kb on + strand, within BBR_RS13890 at 896.579 kb on + strand, within BBR_RS13890 at 896.584 kb on + strand, within BBR_RS13890 at 896.589 kb on + strand, within BBR_RS13890 at 896.600 kb on + strand, within BBR_RS13890 at 896.604 kb on + strand, within BBR_RS13890 at 896.661 kb on + strand, within BBR_RS13890 at 896.720 kb on + strand, within BBR_RS13890 at 896.839 kb on + strand, within BBR_RS13890 at 896.845 kb on + strand, within BBR_RS13890 at 896.902 kb on + strand, within BBR_RS13890 at 896.920 kb on + strand, within BBR_RS13890 at 896.930 kb on + strand, within BBR_RS13890 at 896.976 kb on + strand, within BBR_RS13890
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 894,126 - +0.6 894,128 + +2.0 894,128 - -2.6 894,128 - +0.0 894,164 + +0.5 894,174 - -0.3 894,174 - -0.2 894,174 - +0.5 894,199 - -0.5 894,234 - BBR_RS13880 0.12 -1.0 894,245 - BBR_RS13880 0.14 -0.7 894,245 - BBR_RS13880 0.14 +0.3 894,296 + BBR_RS13880 0.22 +0.7 894,306 - BBR_RS13880 0.23 -1.3 894,345 - BBR_RS13880 0.29 +0.5 894,384 - BBR_RS13880 0.35 -0.1 894,389 + BBR_RS13880 0.36 -0.9 894,397 - BBR_RS13880 0.37 +1.1 894,413 + BBR_RS13880 0.40 -0.3 894,448 + BBR_RS13880 0.45 -0.2 894,483 - BBR_RS13880 0.50 -1.0 894,528 - BBR_RS13880 0.57 -0.3 894,557 - BBR_RS13880 0.62 -0.3 894,557 - BBR_RS13880 0.62 -1.3 894,568 + BBR_RS13880 0.63 +0.2 894,568 + BBR_RS13880 0.63 -0.5 894,604 - BBR_RS13880 0.69 -0.6 894,653 - BBR_RS13880 0.76 +0.2 894,673 + BBR_RS13880 0.79 +0.1 894,682 + BBR_RS13880 0.81 -0.2 894,682 + BBR_RS13880 0.81 -0.8 894,692 - BBR_RS13880 0.82 -0.1 894,698 - BBR_RS13880 0.83 -0.7 894,777 - -0.6 894,798 + +0.1 894,803 + -0.1 894,803 + -0.7 894,808 + +0.1 894,811 - -0.1 894,811 - +1.0 894,833 + +0.9 894,909 - -0.7 894,949 - -0.1 894,999 + -1.8 895,087 + BBR_RS13885 0.17 -3.0 895,087 + BBR_RS13885 0.17 -3.0 895,092 + BBR_RS13885 0.17 -2.1 895,095 - BBR_RS13885 0.17 -1.7 895,095 - BBR_RS13885 0.17 -1.9 895,156 + BBR_RS13885 0.23 -1.5 895,193 + BBR_RS13885 0.26 -1.3 895,281 + BBR_RS13885 0.34 -1.8 895,283 + BBR_RS13885 0.35 +1.5 895,342 + BBR_RS13885 0.40 -0.5 895,342 + BBR_RS13885 0.40 -2.2 895,342 + BBR_RS13885 0.40 -1.8 895,344 - BBR_RS13885 0.40 -0.5 895,371 + BBR_RS13885 0.43 -2.7 895,383 + BBR_RS13885 0.44 -1.7 895,386 + BBR_RS13885 0.44 -1.9 895,415 + BBR_RS13885 0.47 -3.1 895,421 + BBR_RS13885 0.47 -2.2 895,429 - BBR_RS13885 0.48 +0.2 895,465 - BBR_RS13885 0.51 +1.5 895,468 + BBR_RS13885 0.51 -1.2 895,542 - BBR_RS13885 0.58 -1.2 895,542 - BBR_RS13885 0.58 -2.2 895,600 + BBR_RS13885 0.63 -1.4 895,627 + BBR_RS13885 0.66 -1.4 895,663 + BBR_RS13885 0.69 -1.6 895,663 + BBR_RS13885 0.69 -1.5 895,681 - BBR_RS13885 0.71 -2.0 895,708 + BBR_RS13885 0.73 -2.6 895,708 + BBR_RS13885 0.73 -2.5 895,708 + BBR_RS13885 0.73 -2.0 895,708 + BBR_RS13885 0.73 -2.6 895,716 - BBR_RS13885 0.74 -4.2 895,716 - BBR_RS13885 0.74 -2.8 895,723 + BBR_RS13885 0.75 -1.1 895,763 + BBR_RS13885 0.78 +0.9 895,771 - BBR_RS13885 0.79 -1.5 895,771 - BBR_RS13885 0.79 -2.8 895,771 - BBR_RS13885 0.79 +0.9 895,794 - BBR_RS13885 0.81 -0.3 895,806 + BBR_RS13885 0.82 -1.7 895,848 - BBR_RS13885 0.86 -0.7 895,894 + +0.5 895,914 - -2.4 895,949 - -0.8 895,973 - +1.5 895,983 - -1.7 895,986 + -1.5 895,986 + -0.4 895,986 + -0.8 895,986 + -0.7 895,986 + -0.5 895,986 + -0.4 895,988 + -1.2 895,988 + -3.4 895,989 - -0.9 895,989 - -0.8 896,062 + -1.5 896,094 - +2.5 896,112 + -2.3 896,141 + BBR_RS13890 0.12 -2.8 896,141 + BBR_RS13890 0.12 -2.1 896,144 - BBR_RS13890 0.13 -1.1 896,149 + BBR_RS13890 0.13 -3.6 896,149 + BBR_RS13890 0.13 -1.0 896,152 + BBR_RS13890 0.13 -1.3 896,163 + BBR_RS13890 0.14 -1.5 896,170 + BBR_RS13890 0.15 -2.6 896,223 + BBR_RS13890 0.19 -1.2 896,231 + BBR_RS13890 0.20 -3.0 896,236 - BBR_RS13890 0.20 +0.9 896,251 - BBR_RS13890 0.22 +1.5 896,265 + BBR_RS13890 0.23 -2.8 896,265 + BBR_RS13890 0.23 -3.6 896,304 + BBR_RS13890 0.26 -2.6 896,306 + BBR_RS13890 0.26 -2.3 896,321 + BBR_RS13890 0.28 -1.2 896,326 + BBR_RS13890 0.28 -2.8 896,409 + BBR_RS13890 0.35 -2.1 896,414 + BBR_RS13890 0.36 +0.1 896,425 + BBR_RS13890 0.36 -2.7 896,462 + BBR_RS13890 0.40 -1.4 896,462 + BBR_RS13890 0.40 -1.8 896,464 + BBR_RS13890 0.40 -1.4 896,472 + BBR_RS13890 0.40 -2.2 896,506 + BBR_RS13890 0.43 -2.4 896,513 - BBR_RS13890 0.44 -1.3 896,540 + BBR_RS13890 0.46 -2.0 896,550 - BBR_RS13890 0.47 +0.9 896,569 + BBR_RS13890 0.49 -2.8 896,574 + BBR_RS13890 0.49 -1.3 896,574 + BBR_RS13890 0.49 -0.1 896,579 + BBR_RS13890 0.50 -1.4 896,584 + BBR_RS13890 0.50 -2.0 896,589 + BBR_RS13890 0.50 -2.0 896,600 + BBR_RS13890 0.51 -2.1 896,604 + BBR_RS13890 0.52 -1.5 896,661 + BBR_RS13890 0.57 -0.4 896,720 + BBR_RS13890 0.62 -2.0 896,839 + BBR_RS13890 0.72 -3.0 896,845 + BBR_RS13890 0.72 -2.0 896,902 + BBR_RS13890 0.77 -1.4 896,920 + BBR_RS13890 0.79 +0.3 896,930 + BBR_RS13890 0.79 -4.3 896,976 + BBR_RS13890 0.83 -1.8
Or see this region's nucleotide sequence