Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12830 and BBR_RS12835 are separated by 172 nucleotides BBR_RS12835 and BBR_RS20730 are separated by 13 nucleotides BBR_RS20730 and BBR_RS12840 overlap by 2 nucleotides BBR_RS12840 and BBR_RS12845 are separated by 134 nucleotides
BBR_RS12830: BBR_RS12830 - G5 domain-containing protein, at 628,568 to 629,551
_RS12830
BBR_RS12835: BBR_RS12835 - thioredoxin, at 629,724 to 630,095
_RS12835
BBR_RS20730: BBR_RS20730 - Crp/Fnr family transcriptional regulator, at 630,109 to 630,294
_RS20730
BBR_RS12840: BBR_RS12840 - DUF4916 domain-containing protein, at 630,293 to 630,925
_RS12840
BBR_RS12845: BBR_RS12845 - alpha/beta hydrolase, at 631,060 to 631,905
_RS12845
Position (kb) 630
631 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 629.317 kb on + strand, within BBR_RS12830 at 629.323 kb on - strand, within BBR_RS12830 at 629.325 kb on - strand, within BBR_RS12830 at 629.335 kb on - strand, within BBR_RS12830 at 629.386 kb on + strand, within BBR_RS12830 at 629.396 kb on + strand, within BBR_RS12830 at 629.404 kb on + strand, within BBR_RS12830 at 629.404 kb on + strand, within BBR_RS12830 at 629.404 kb on + strand, within BBR_RS12830 at 629.412 kb on + strand, within BBR_RS12830 at 629.412 kb on - strand, within BBR_RS12830 at 629.420 kb on - strand, within BBR_RS12830 at 629.443 kb on + strand, within BBR_RS12830 at 629.445 kb on - strand, within BBR_RS12830 at 629.558 kb on + strand at 629.558 kb on + strand at 629.567 kb on + strand at 629.567 kb on + strand at 629.567 kb on + strand at 629.568 kb on + strand at 629.575 kb on - strand at 629.575 kb on - strand at 629.575 kb on - strand at 629.575 kb on - strand at 629.575 kb on - strand at 629.575 kb on - strand at 629.577 kb on + strand at 629.654 kb on + strand at 629.721 kb on + strand at 629.722 kb on + strand at 629.741 kb on - strand at 629.776 kb on + strand, within BBR_RS12835 at 629.776 kb on - strand, within BBR_RS12835 at 629.784 kb on - strand, within BBR_RS12835 at 629.784 kb on - strand, within BBR_RS12835 at 629.784 kb on - strand, within BBR_RS12835 at 629.784 kb on - strand, within BBR_RS12835 at 629.819 kb on - strand, within BBR_RS12835 at 629.839 kb on - strand, within BBR_RS12835 at 629.842 kb on + strand, within BBR_RS12835 at 629.854 kb on - strand, within BBR_RS12835 at 629.863 kb on - strand, within BBR_RS12835 at 629.997 kb on + strand, within BBR_RS12835 at 629.997 kb on + strand, within BBR_RS12835 at 629.997 kb on + strand, within BBR_RS12835 at 630.002 kb on + strand, within BBR_RS12835 at 630.002 kb on + strand, within BBR_RS12835 at 630.002 kb on + strand, within BBR_RS12835 at 630.005 kb on - strand, within BBR_RS12835 at 630.011 kb on + strand, within BBR_RS12835 at 630.024 kb on - strand, within BBR_RS12835 at 630.024 kb on - strand, within BBR_RS12835 at 630.068 kb on + strand at 630.118 kb on + strand at 630.120 kb on + strand at 630.120 kb on + strand at 630.126 kb on - strand at 630.128 kb on - strand, within BBR_RS20730 at 630.130 kb on + strand, within BBR_RS20730 at 630.130 kb on + strand, within BBR_RS20730 at 630.138 kb on - strand, within BBR_RS20730 at 630.141 kb on + strand, within BBR_RS20730 at 630.197 kb on + strand, within BBR_RS20730 at 630.202 kb on + strand, within BBR_RS20730 at 630.210 kb on - strand, within BBR_RS20730 at 630.265 kb on - strand, within BBR_RS20730 at 630.271 kb on - strand, within BBR_RS20730 at 630.284 kb on + strand at 630.294 kb on + strand at 630.323 kb on - strand at 630.367 kb on + strand, within BBR_RS12840 at 630.388 kb on - strand, within BBR_RS12840 at 630.399 kb on + strand, within BBR_RS12840 at 630.407 kb on - strand, within BBR_RS12840 at 630.416 kb on + strand, within BBR_RS12840 at 630.416 kb on + strand, within BBR_RS12840 at 630.416 kb on + strand, within BBR_RS12840 at 630.434 kb on - strand, within BBR_RS12840 at 630.440 kb on + strand, within BBR_RS12840 at 630.478 kb on + strand, within BBR_RS12840 at 630.483 kb on + strand, within BBR_RS12840 at 630.524 kb on - strand, within BBR_RS12840 at 630.534 kb on - strand, within BBR_RS12840 at 630.558 kb on + strand, within BBR_RS12840 at 630.566 kb on + strand, within BBR_RS12840 at 630.593 kb on - strand, within BBR_RS12840 at 630.624 kb on + strand, within BBR_RS12840 at 630.675 kb on + strand, within BBR_RS12840 at 630.680 kb on + strand, within BBR_RS12840 at 630.685 kb on + strand, within BBR_RS12840 at 630.685 kb on + strand, within BBR_RS12840 at 630.686 kb on - strand, within BBR_RS12840 at 630.687 kb on + strand, within BBR_RS12840 at 630.693 kb on - strand, within BBR_RS12840 at 630.706 kb on - strand, within BBR_RS12840 at 630.714 kb on + strand, within BBR_RS12840 at 630.722 kb on - strand, within BBR_RS12840 at 630.732 kb on + strand, within BBR_RS12840 at 630.763 kb on + strand, within BBR_RS12840 at 630.763 kb on + strand, within BBR_RS12840 at 630.763 kb on + strand, within BBR_RS12840 at 630.765 kb on + strand, within BBR_RS12840 at 630.765 kb on + strand, within BBR_RS12840 at 630.771 kb on - strand, within BBR_RS12840 at 630.802 kb on + strand, within BBR_RS12840 at 630.810 kb on - strand, within BBR_RS12840 at 630.825 kb on + strand, within BBR_RS12840 at 630.825 kb on + strand, within BBR_RS12840 at 630.830 kb on + strand, within BBR_RS12840 at 630.830 kb on + strand, within BBR_RS12840 at 630.830 kb on + strand, within BBR_RS12840 at 630.830 kb on + strand, within BBR_RS12840 at 630.838 kb on - strand, within BBR_RS12840 at 630.838 kb on - strand, within BBR_RS12840 at 630.840 kb on - strand, within BBR_RS12840 at 630.855 kb on - strand, within BBR_RS12840 at 630.879 kb on + strand at 630.907 kb on + strand at 630.943 kb on + strand at 630.951 kb on - strand at 630.959 kb on - strand at 630.971 kb on - strand at 631.011 kb on + strand at 631.035 kb on + strand at 631.051 kb on - strand at 631.077 kb on - strand at 631.119 kb on + strand at 631.127 kb on - strand at 631.148 kb on + strand, within BBR_RS12845 at 631.154 kb on - strand, within BBR_RS12845 at 631.160 kb on + strand, within BBR_RS12845 at 631.160 kb on + strand, within BBR_RS12845 at 631.160 kb on + strand, within BBR_RS12845 at 631.160 kb on + strand, within BBR_RS12845 at 631.168 kb on - strand, within BBR_RS12845 at 631.168 kb on - strand, within BBR_RS12845 at 631.207 kb on + strand, within BBR_RS12845 at 631.255 kb on - strand, within BBR_RS12845 at 631.276 kb on - strand, within BBR_RS12845 at 631.286 kb on - strand, within BBR_RS12845 at 631.299 kb on + strand, within BBR_RS12845 at 631.299 kb on + strand, within BBR_RS12845 at 631.314 kb on - strand, within BBR_RS12845 at 631.321 kb on + strand, within BBR_RS12845 at 631.331 kb on + strand, within BBR_RS12845 at 631.335 kb on - strand, within BBR_RS12845 at 631.335 kb on - strand, within BBR_RS12845 at 631.341 kb on + strand, within BBR_RS12845 at 631.341 kb on + strand, within BBR_RS12845 at 631.349 kb on - strand, within BBR_RS12845 at 631.349 kb on - strand, within BBR_RS12845 at 631.354 kb on + strand, within BBR_RS12845 at 631.383 kb on + strand, within BBR_RS12845 at 631.383 kb on + strand, within BBR_RS12845 at 631.394 kb on + strand, within BBR_RS12845 at 631.402 kb on - strand, within BBR_RS12845 at 631.442 kb on + strand, within BBR_RS12845 at 631.442 kb on + strand, within BBR_RS12845 at 631.452 kb on + strand, within BBR_RS12845 at 631.454 kb on - strand, within BBR_RS12845 at 631.454 kb on - strand, within BBR_RS12845 at 631.501 kb on + strand, within BBR_RS12845 at 631.529 kb on + strand, within BBR_RS12845 at 631.532 kb on - strand, within BBR_RS12845 at 631.553 kb on + strand, within BBR_RS12845 at 631.582 kb on - strand, within BBR_RS12845 at 631.606 kb on - strand, within BBR_RS12845 at 631.616 kb on - strand, within BBR_RS12845 at 631.622 kb on + strand, within BBR_RS12845 at 631.630 kb on - strand, within BBR_RS12845 at 631.630 kb on - strand, within BBR_RS12845 at 631.645 kb on + strand, within BBR_RS12845 at 631.646 kb on + strand, within BBR_RS12845 at 631.653 kb on - strand, within BBR_RS12845 at 631.658 kb on - strand, within BBR_RS12845 at 631.658 kb on - strand, within BBR_RS12845 at 631.669 kb on + strand, within BBR_RS12845 at 631.669 kb on + strand, within BBR_RS12845 at 631.670 kb on - strand, within BBR_RS12845 at 631.677 kb on - strand, within BBR_RS12845 at 631.677 kb on - strand, within BBR_RS12845 at 631.677 kb on - strand, within BBR_RS12845 at 631.677 kb on - strand, within BBR_RS12845 at 631.677 kb on - strand, within BBR_RS12845 at 631.684 kb on + strand, within BBR_RS12845 at 631.698 kb on + strand, within BBR_RS12845 at 631.706 kb on + strand, within BBR_RS12845 at 631.714 kb on - strand, within BBR_RS12845 at 631.716 kb on + strand, within BBR_RS12845 at 631.736 kb on - strand, within BBR_RS12845 at 631.763 kb on - strand, within BBR_RS12845 at 631.775 kb on + strand, within BBR_RS12845 at 631.830 kb on + strand at 631.838 kb on - strand at 631.846 kb on + strand at 631.848 kb on + strand at 631.848 kb on + strand at 631.856 kb on - strand at 631.856 kb on - strand at 631.856 kb on - strand at 631.881 kb on + strand at 631.889 kb on - strand at 631.889 kb on - strand at 631.889 kb on - strand at 631.894 kb on + strand at 631.897 kb on + strand at 631.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 629,317 + BBR_RS12830 0.76 +0.1 629,323 - BBR_RS12830 0.77 -0.7 629,325 - BBR_RS12830 0.77 -1.6 629,335 - BBR_RS12830 0.78 +0.6 629,386 + BBR_RS12830 0.83 -0.8 629,396 + BBR_RS12830 0.84 -0.6 629,404 + BBR_RS12830 0.85 -0.2 629,404 + BBR_RS12830 0.85 -0.3 629,404 + BBR_RS12830 0.85 -0.4 629,412 + BBR_RS12830 0.86 -0.6 629,412 - BBR_RS12830 0.86 -0.2 629,420 - BBR_RS12830 0.87 -0.4 629,443 + BBR_RS12830 0.89 -0.2 629,445 - BBR_RS12830 0.89 -0.1 629,558 + -0.2 629,558 + -1.0 629,567 + -0.2 629,567 + -0.9 629,567 + +0.2 629,568 + -0.6 629,575 - -0.1 629,575 - +0.1 629,575 - +0.5 629,575 - +0.2 629,575 - +0.8 629,575 - +0.7 629,577 + -1.3 629,654 + -1.9 629,721 + -2.0 629,722 + -1.4 629,741 - -1.3 629,776 + BBR_RS12835 0.14 -1.8 629,776 - BBR_RS12835 0.14 -1.7 629,784 - BBR_RS12835 0.16 -1.7 629,784 - BBR_RS12835 0.16 -2.1 629,784 - BBR_RS12835 0.16 -0.1 629,784 - BBR_RS12835 0.16 -1.0 629,819 - BBR_RS12835 0.26 -1.1 629,839 - BBR_RS12835 0.31 -1.2 629,842 + BBR_RS12835 0.32 -0.9 629,854 - BBR_RS12835 0.35 -1.7 629,863 - BBR_RS12835 0.37 -0.7 629,997 + BBR_RS12835 0.73 -1.1 629,997 + BBR_RS12835 0.73 -2.4 629,997 + BBR_RS12835 0.73 -2.6 630,002 + BBR_RS12835 0.75 -3.6 630,002 + BBR_RS12835 0.75 -3.9 630,002 + BBR_RS12835 0.75 -3.6 630,005 - BBR_RS12835 0.76 +0.1 630,011 + BBR_RS12835 0.77 -1.7 630,024 - BBR_RS12835 0.81 -2.0 630,024 - BBR_RS12835 0.81 -0.7 630,068 + -2.5 630,118 + -2.1 630,120 + -1.6 630,120 + -1.9 630,126 - -1.9 630,128 - BBR_RS20730 0.10 -1.9 630,130 + BBR_RS20730 0.11 -0.7 630,130 + BBR_RS20730 0.11 -2.2 630,138 - BBR_RS20730 0.16 +0.1 630,141 + BBR_RS20730 0.17 -1.4 630,197 + BBR_RS20730 0.47 -0.1 630,202 + BBR_RS20730 0.50 -0.4 630,210 - BBR_RS20730 0.54 -0.0 630,265 - BBR_RS20730 0.84 -0.7 630,271 - BBR_RS20730 0.87 -2.3 630,284 + -0.7 630,294 + -0.4 630,323 - -0.7 630,367 + BBR_RS12840 0.12 -0.2 630,388 - BBR_RS12840 0.15 -3.4 630,399 + BBR_RS12840 0.17 -2.4 630,407 - BBR_RS12840 0.18 -3.3 630,416 + BBR_RS12840 0.19 +0.2 630,416 + BBR_RS12840 0.19 -2.1 630,416 + BBR_RS12840 0.19 -2.2 630,434 - BBR_RS12840 0.22 -1.5 630,440 + BBR_RS12840 0.23 +0.1 630,478 + BBR_RS12840 0.29 -1.9 630,483 + BBR_RS12840 0.30 -1.4 630,524 - BBR_RS12840 0.36 -2.0 630,534 - BBR_RS12840 0.38 -2.1 630,558 + BBR_RS12840 0.42 -3.0 630,566 + BBR_RS12840 0.43 -1.6 630,593 - BBR_RS12840 0.47 -2.0 630,624 + BBR_RS12840 0.52 -0.9 630,675 + BBR_RS12840 0.60 -3.2 630,680 + BBR_RS12840 0.61 -1.2 630,685 + BBR_RS12840 0.62 -1.7 630,685 + BBR_RS12840 0.62 -3.2 630,686 - BBR_RS12840 0.62 -2.5 630,687 + BBR_RS12840 0.62 -3.5 630,693 - BBR_RS12840 0.63 -1.8 630,706 - BBR_RS12840 0.65 +0.1 630,714 + BBR_RS12840 0.67 -1.5 630,722 - BBR_RS12840 0.68 -1.7 630,732 + BBR_RS12840 0.69 -0.9 630,763 + BBR_RS12840 0.74 -1.4 630,763 + BBR_RS12840 0.74 -2.7 630,763 + BBR_RS12840 0.74 -2.1 630,765 + BBR_RS12840 0.75 -2.2 630,765 + BBR_RS12840 0.75 +1.0 630,771 - BBR_RS12840 0.76 +0.5 630,802 + BBR_RS12840 0.80 -1.5 630,810 - BBR_RS12840 0.82 -2.4 630,825 + BBR_RS12840 0.84 -2.0 630,825 + BBR_RS12840 0.84 -2.2 630,830 + BBR_RS12840 0.85 -1.1 630,830 + BBR_RS12840 0.85 -1.5 630,830 + BBR_RS12840 0.85 -2.2 630,830 + BBR_RS12840 0.85 -2.9 630,838 - BBR_RS12840 0.86 -2.4 630,838 - BBR_RS12840 0.86 -2.2 630,840 - BBR_RS12840 0.86 -2.0 630,855 - BBR_RS12840 0.89 -3.7 630,879 + +0.1 630,907 + -4.3 630,943 + -0.4 630,951 - -0.8 630,959 - -2.8 630,971 - -1.4 631,011 + -1.1 631,035 + -0.2 631,051 - -2.0 631,077 - -1.9 631,119 + +0.1 631,127 - -1.0 631,148 + BBR_RS12845 0.10 -0.6 631,154 - BBR_RS12845 0.11 -0.3 631,160 + BBR_RS12845 0.12 +0.3 631,160 + BBR_RS12845 0.12 -0.5 631,160 + BBR_RS12845 0.12 -0.9 631,160 + BBR_RS12845 0.12 +0.8 631,168 - BBR_RS12845 0.13 -1.5 631,168 - BBR_RS12845 0.13 -1.5 631,207 + BBR_RS12845 0.17 +1.2 631,255 - BBR_RS12845 0.23 +0.1 631,276 - BBR_RS12845 0.26 -2.5 631,286 - BBR_RS12845 0.27 -2.4 631,299 + BBR_RS12845 0.28 +0.6 631,299 + BBR_RS12845 0.28 +0.2 631,314 - BBR_RS12845 0.30 -1.8 631,321 + BBR_RS12845 0.31 -0.5 631,331 + BBR_RS12845 0.32 -1.2 631,335 - BBR_RS12845 0.33 -1.7 631,335 - BBR_RS12845 0.33 -0.7 631,341 + BBR_RS12845 0.33 -1.8 631,341 + BBR_RS12845 0.33 +1.3 631,349 - BBR_RS12845 0.34 -1.2 631,349 - BBR_RS12845 0.34 -0.2 631,354 + BBR_RS12845 0.35 -0.8 631,383 + BBR_RS12845 0.38 -1.9 631,383 + BBR_RS12845 0.38 +0.9 631,394 + BBR_RS12845 0.39 -0.1 631,402 - BBR_RS12845 0.40 -1.5 631,442 + BBR_RS12845 0.45 +0.4 631,442 + BBR_RS12845 0.45 +0.2 631,452 + BBR_RS12845 0.46 +0.2 631,454 - BBR_RS12845 0.47 +0.1 631,454 - BBR_RS12845 0.47 +0.1 631,501 + BBR_RS12845 0.52 +0.2 631,529 + BBR_RS12845 0.55 +0.5 631,532 - BBR_RS12845 0.56 -1.0 631,553 + BBR_RS12845 0.58 -0.1 631,582 - BBR_RS12845 0.62 -1.0 631,606 - BBR_RS12845 0.65 -1.0 631,616 - BBR_RS12845 0.66 -0.5 631,622 + BBR_RS12845 0.66 +0.9 631,630 - BBR_RS12845 0.67 -1.7 631,630 - BBR_RS12845 0.67 -1.7 631,645 + BBR_RS12845 0.69 -0.3 631,646 + BBR_RS12845 0.69 -0.9 631,653 - BBR_RS12845 0.70 -0.1 631,658 - BBR_RS12845 0.71 -0.1 631,658 - BBR_RS12845 0.71 -0.8 631,669 + BBR_RS12845 0.72 -0.8 631,669 + BBR_RS12845 0.72 -0.7 631,670 - BBR_RS12845 0.72 -1.7 631,677 - BBR_RS12845 0.73 -1.4 631,677 - BBR_RS12845 0.73 +0.1 631,677 - BBR_RS12845 0.73 -1.2 631,677 - BBR_RS12845 0.73 -2.3 631,677 - BBR_RS12845 0.73 -0.9 631,684 + BBR_RS12845 0.74 +0.2 631,698 + BBR_RS12845 0.75 +0.4 631,706 + BBR_RS12845 0.76 -0.6 631,714 - BBR_RS12845 0.77 -0.1 631,716 + BBR_RS12845 0.78 +0.2 631,736 - BBR_RS12845 0.80 +0.0 631,763 - BBR_RS12845 0.83 -1.1 631,775 + BBR_RS12845 0.85 -0.8 631,830 + +0.5 631,838 - +0.9 631,846 + -0.0 631,848 + +0.6 631,848 + -0.8 631,856 - -0.0 631,856 - -1.3 631,856 - -0.7 631,881 + +0.9 631,889 - -0.7 631,889 - +0.3 631,889 - -1.9 631,894 + +2.1 631,897 + -0.1 631,918 + +0.6
Or see this region's nucleotide sequence