Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10545 and BBR_RS10550 are separated by 534 nucleotides BBR_RS10550 and BBR_RS10555 are separated by 133 nucleotides BBR_RS10555 and BBR_RS10560 are separated by 212 nucleotides
BBR_RS10545: BBR_RS10545 - amidohydrolase, at 82,549 to 84,168
_RS10545
BBR_RS10550: BBR_RS10550 - tryptophan--tRNA ligase, at 84,703 to 85,794
_RS10550
BBR_RS10555: BBR_RS10555 - DUF3073 domain-containing protein, at 85,928 to 86,311
_RS10555
BBR_RS10560: BBR_RS10560 - hypothetical protein, at 86,524 to 87,093
_RS10560
Position (kb) 84
85
86 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 83.720 kb on + strand, within BBR_RS10545 at 83.720 kb on + strand, within BBR_RS10545 at 83.720 kb on - strand, within BBR_RS10545 at 83.720 kb on - strand, within BBR_RS10545 at 83.720 kb on - strand, within BBR_RS10545 at 83.720 kb on - strand, within BBR_RS10545 at 83.720 kb on - strand, within BBR_RS10545 at 83.723 kb on + strand, within BBR_RS10545 at 83.731 kb on - strand, within BBR_RS10545 at 83.754 kb on - strand, within BBR_RS10545 at 83.762 kb on + strand, within BBR_RS10545 at 83.778 kb on + strand, within BBR_RS10545 at 83.779 kb on + strand, within BBR_RS10545 at 83.781 kb on - strand, within BBR_RS10545 at 83.788 kb on - strand, within BBR_RS10545 at 83.788 kb on - strand, within BBR_RS10545 at 83.796 kb on + strand, within BBR_RS10545 at 83.805 kb on - strand, within BBR_RS10545 at 83.805 kb on - strand, within BBR_RS10545 at 83.805 kb on - strand, within BBR_RS10545 at 83.816 kb on - strand, within BBR_RS10545 at 83.816 kb on - strand, within BBR_RS10545 at 83.832 kb on + strand, within BBR_RS10545 at 83.840 kb on - strand, within BBR_RS10545 at 83.840 kb on - strand, within BBR_RS10545 at 83.849 kb on - strand, within BBR_RS10545 at 83.849 kb on - strand, within BBR_RS10545 at 83.859 kb on - strand, within BBR_RS10545 at 83.859 kb on - strand, within BBR_RS10545 at 83.864 kb on + strand, within BBR_RS10545 at 83.872 kb on - strand, within BBR_RS10545 at 83.874 kb on + strand, within BBR_RS10545 at 83.874 kb on + strand, within BBR_RS10545 at 83.881 kb on + strand, within BBR_RS10545 at 83.893 kb on + strand, within BBR_RS10545 at 83.893 kb on + strand, within BBR_RS10545 at 83.893 kb on + strand, within BBR_RS10545 at 83.893 kb on + strand, within BBR_RS10545 at 83.898 kb on + strand, within BBR_RS10545 at 83.901 kb on - strand, within BBR_RS10545 at 83.901 kb on - strand, within BBR_RS10545 at 83.903 kb on + strand, within BBR_RS10545 at 83.910 kb on - strand, within BBR_RS10545 at 83.911 kb on - strand, within BBR_RS10545 at 83.917 kb on - strand, within BBR_RS10545 at 83.935 kb on + strand, within BBR_RS10545 at 83.967 kb on + strand, within BBR_RS10545 at 83.967 kb on + strand, within BBR_RS10545 at 83.967 kb on + strand, within BBR_RS10545 at 83.967 kb on + strand, within BBR_RS10545 at 83.972 kb on + strand, within BBR_RS10545 at 83.975 kb on - strand, within BBR_RS10545 at 83.975 kb on - strand, within BBR_RS10545 at 83.975 kb on - strand, within BBR_RS10545 at 83.982 kb on + strand, within BBR_RS10545 at 83.984 kb on + strand, within BBR_RS10545 at 83.984 kb on + strand, within BBR_RS10545 at 83.984 kb on + strand, within BBR_RS10545 at 83.984 kb on + strand, within BBR_RS10545 at 83.992 kb on + strand, within BBR_RS10545 at 83.992 kb on + strand, within BBR_RS10545 at 83.992 kb on - strand, within BBR_RS10545 at 83.992 kb on - strand, within BBR_RS10545 at 83.999 kb on + strand, within BBR_RS10545 at 83.999 kb on + strand, within BBR_RS10545 at 83.999 kb on + strand, within BBR_RS10545 at 84.000 kb on - strand, within BBR_RS10545 at 84.007 kb on - strand at 84.009 kb on + strand at 84.009 kb on + strand at 84.017 kb on + strand at 84.026 kb on + strand at 84.026 kb on + strand at 84.026 kb on + strand at 84.034 kb on - strand at 84.064 kb on + strand at 84.071 kb on - strand at 84.072 kb on - strand at 84.074 kb on + strand at 84.077 kb on - strand at 84.077 kb on - strand at 84.079 kb on + strand at 84.081 kb on + strand at 84.081 kb on + strand at 84.082 kb on - strand at 84.082 kb on - strand at 84.082 kb on - strand at 84.087 kb on - strand at 84.087 kb on - strand at 84.087 kb on - strand at 84.089 kb on - strand at 84.089 kb on - strand at 84.089 kb on - strand at 84.093 kb on + strand at 84.100 kb on + strand at 84.100 kb on + strand at 84.100 kb on + strand at 84.106 kb on + strand at 84.106 kb on - strand at 84.106 kb on - strand at 84.106 kb on - strand at 84.106 kb on - strand at 84.108 kb on - strand at 84.157 kb on + strand at 84.157 kb on + strand at 84.165 kb on - strand at 84.165 kb on - strand at 84.165 kb on - strand at 84.168 kb on + strand at 84.168 kb on + strand at 84.170 kb on - strand at 84.181 kb on + strand at 84.199 kb on + strand at 84.210 kb on + strand at 84.305 kb on + strand at 84.313 kb on - strand at 84.313 kb on - strand at 84.323 kb on + strand at 84.323 kb on - strand at 84.336 kb on + strand at 84.336 kb on + strand at 84.341 kb on + strand at 84.348 kb on + strand at 84.356 kb on - strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.360 kb on - strand at 84.360 kb on - strand at 84.362 kb on + strand at 84.362 kb on + strand at 84.362 kb on + strand at 84.365 kb on - strand at 84.365 kb on - strand at 84.370 kb on - strand at 84.392 kb on + strand at 84.392 kb on + strand at 84.392 kb on + strand at 84.394 kb on + strand at 84.414 kb on + strand at 84.414 kb on + strand at 84.421 kb on - strand at 84.450 kb on - strand at 84.463 kb on - strand at 84.491 kb on - strand at 84.505 kb on - strand at 84.546 kb on + strand at 84.554 kb on - strand at 84.554 kb on - strand at 84.554 kb on - strand at 84.564 kb on + strand at 84.567 kb on - strand at 84.571 kb on - strand at 84.572 kb on - strand at 84.588 kb on - strand at 84.592 kb on + strand at 84.592 kb on + strand at 84.599 kb on + strand at 84.601 kb on + strand at 84.661 kb on + strand at 84.671 kb on - strand at 84.671 kb on - strand at 84.686 kb on + strand at 84.686 kb on - strand at 84.709 kb on - strand at 84.714 kb on + strand at 85.777 kb on - strand at 85.810 kb on - strand at 85.810 kb on - strand at 85.867 kb on + strand at 85.873 kb on + strand at 85.875 kb on - strand at 85.875 kb on - strand at 85.879 kb on + strand at 85.881 kb on - strand at 85.904 kb on + strand at 85.920 kb on + strand at 86.052 kb on + strand, within BBR_RS10555 at 86.235 kb on + strand, within BBR_RS10555 at 86.275 kb on + strand at 86.275 kb on + strand at 86.283 kb on - strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.315 kb on - strand at 86.315 kb on - strand at 86.317 kb on + strand at 86.325 kb on - strand at 86.351 kb on - strand at 86.352 kb on + strand at 86.361 kb on + strand at 86.361 kb on + strand at 86.361 kb on - strand at 86.369 kb on - strand at 86.373 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.425 kb on - strand at 86.425 kb on - strand at 86.440 kb on - strand at 86.447 kb on + strand at 86.475 kb on + strand at 86.493 kb on + strand at 86.520 kb on - strand at 86.530 kb on - strand at 86.535 kb on - strand at 86.536 kb on - strand at 86.559 kb on + strand at 86.567 kb on + strand at 86.572 kb on + strand at 86.575 kb on - strand at 86.580 kb on - strand at 86.580 kb on - strand at 86.586 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.600 kb on + strand, within BBR_RS10560 at 86.606 kb on - strand, within BBR_RS10560 at 86.608 kb on - strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.617 kb on + strand, within BBR_RS10560 at 86.619 kb on - strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.664 kb on + strand, within BBR_RS10560 at 86.664 kb on - strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.669 kb on + strand, within BBR_RS10560 at 86.670 kb on + strand, within BBR_RS10560 at 86.672 kb on - strand, within BBR_RS10560 at 86.673 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.685 kb on + strand, within BBR_RS10560 at 86.687 kb on - strand, within BBR_RS10560 at 86.719 kb on - strand, within BBR_RS10560 at 86.724 kb on - strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.734 kb on - strand, within BBR_RS10560 at 86.741 kb on - strand, within BBR_RS10560 at 86.747 kb on - strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.789 kb on + strand, within BBR_RS10560 at 86.794 kb on + strand, within BBR_RS10560
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 83,720 + BBR_RS10545 0.72 -0.7 83,720 + BBR_RS10545 0.72 +0.4 83,720 - BBR_RS10545 0.72 -0.2 83,720 - BBR_RS10545 0.72 +0.2 83,720 - BBR_RS10545 0.72 -1.0 83,720 - BBR_RS10545 0.72 -0.3 83,720 - BBR_RS10545 0.72 +0.3 83,723 + BBR_RS10545 0.72 -0.4 83,731 - BBR_RS10545 0.73 -0.0 83,754 - BBR_RS10545 0.74 -0.5 83,762 + BBR_RS10545 0.75 -0.1 83,778 + BBR_RS10545 0.76 -0.3 83,779 + BBR_RS10545 0.76 +0.2 83,781 - BBR_RS10545 0.76 +0.4 83,788 - BBR_RS10545 0.76 -0.3 83,788 - BBR_RS10545 0.76 -0.3 83,796 + BBR_RS10545 0.77 +0.3 83,805 - BBR_RS10545 0.78 -0.3 83,805 - BBR_RS10545 0.78 -0.5 83,805 - BBR_RS10545 0.78 +0.4 83,816 - BBR_RS10545 0.78 -0.3 83,816 - BBR_RS10545 0.78 -0.1 83,832 + BBR_RS10545 0.79 -0.8 83,840 - BBR_RS10545 0.80 +0.5 83,840 - BBR_RS10545 0.80 -0.7 83,849 - BBR_RS10545 0.80 +0.0 83,849 - BBR_RS10545 0.80 -0.8 83,859 - BBR_RS10545 0.81 -1.3 83,859 - BBR_RS10545 0.81 -0.6 83,864 + BBR_RS10545 0.81 -1.7 83,872 - BBR_RS10545 0.82 +0.5 83,874 + BBR_RS10545 0.82 +0.8 83,874 + BBR_RS10545 0.82 -0.3 83,881 + BBR_RS10545 0.82 -1.4 83,893 + BBR_RS10545 0.83 +0.9 83,893 + BBR_RS10545 0.83 -1.6 83,893 + BBR_RS10545 0.83 -0.5 83,893 + BBR_RS10545 0.83 +0.7 83,898 + BBR_RS10545 0.83 -1.0 83,901 - BBR_RS10545 0.83 -1.0 83,901 - BBR_RS10545 0.83 +0.0 83,903 + BBR_RS10545 0.84 -0.6 83,910 - BBR_RS10545 0.84 +0.3 83,911 - BBR_RS10545 0.84 -0.7 83,917 - BBR_RS10545 0.84 +0.4 83,935 + BBR_RS10545 0.86 -0.7 83,967 + BBR_RS10545 0.88 +0.0 83,967 + BBR_RS10545 0.88 -0.5 83,967 + BBR_RS10545 0.88 -1.3 83,967 + BBR_RS10545 0.88 -0.8 83,972 + BBR_RS10545 0.88 +0.3 83,975 - BBR_RS10545 0.88 -0.5 83,975 - BBR_RS10545 0.88 -0.6 83,975 - BBR_RS10545 0.88 +0.1 83,982 + BBR_RS10545 0.88 -0.1 83,984 + BBR_RS10545 0.89 -0.3 83,984 + BBR_RS10545 0.89 -1.0 83,984 + BBR_RS10545 0.89 -0.6 83,984 + BBR_RS10545 0.89 -0.2 83,992 + BBR_RS10545 0.89 +0.3 83,992 + BBR_RS10545 0.89 +0.1 83,992 - BBR_RS10545 0.89 -0.3 83,992 - BBR_RS10545 0.89 -0.8 83,999 + BBR_RS10545 0.90 -0.1 83,999 + BBR_RS10545 0.90 -0.1 83,999 + BBR_RS10545 0.90 -1.3 84,000 - BBR_RS10545 0.90 -0.1 84,007 - +0.1 84,009 + -0.2 84,009 + -0.5 84,017 + -0.3 84,026 + -0.8 84,026 + +0.0 84,026 + +1.5 84,034 - -0.6 84,064 + -0.0 84,071 - +0.3 84,072 - +0.8 84,074 + -0.1 84,077 - -0.0 84,077 - -0.4 84,079 + -2.5 84,081 + +0.7 84,081 + +0.9 84,082 - -0.2 84,082 - -0.7 84,082 - +0.2 84,087 - +0.4 84,087 - -0.8 84,087 - +0.1 84,089 - +0.5 84,089 - -0.1 84,089 - -0.8 84,093 + +1.0 84,100 + +0.9 84,100 + +0.2 84,100 + -0.1 84,106 + -0.1 84,106 - +0.8 84,106 - -1.2 84,106 - +0.6 84,106 - -0.5 84,108 - -0.2 84,157 + -0.2 84,157 + -0.4 84,165 - +0.7 84,165 - -0.7 84,165 - +0.5 84,168 + +0.1 84,168 + -0.1 84,170 - +0.0 84,181 + -0.4 84,199 + +0.4 84,210 + -0.3 84,305 + -2.5 84,313 - -1.4 84,313 - -0.9 84,323 + -1.4 84,323 - -0.7 84,336 + -1.1 84,336 + +0.2 84,341 + +0.3 84,348 + -1.4 84,356 - -0.9 84,357 + -1.9 84,357 + -0.7 84,360 - -0.2 84,360 - -0.2 84,362 + -0.7 84,362 + -1.0 84,362 + -0.2 84,365 - -0.1 84,365 - -1.1 84,370 - -0.7 84,392 + -1.3 84,392 + -0.5 84,392 + -1.1 84,394 + -0.6 84,414 + -1.7 84,414 + +0.1 84,421 - -1.4 84,450 - -1.1 84,463 - +0.2 84,491 - +0.3 84,505 - +1.3 84,546 + +0.1 84,554 - -0.2 84,554 - +0.3 84,554 - +0.1 84,564 + -0.7 84,567 - -0.5 84,571 - -1.1 84,572 - +0.1 84,588 - +0.1 84,592 + +0.0 84,592 + -0.8 84,599 + -1.8 84,601 + -0.2 84,661 + -0.4 84,671 - +0.0 84,671 - +0.1 84,686 + -1.3 84,686 - +0.0 84,709 - +0.6 84,714 + -3.4 85,777 - -0.0 85,810 - -0.2 85,810 - -0.1 85,867 + +0.2 85,873 + -3.5 85,875 - -1.9 85,875 - -4.0 85,879 + -2.5 85,881 - -0.7 85,904 + -2.8 85,920 + -0.5 86,052 + BBR_RS10555 0.32 -3.4 86,235 + BBR_RS10555 0.80 -4.6 86,275 + -1.9 86,275 + -2.1 86,283 - -1.7 86,296 + -4.2 86,296 + -3.8 86,296 + -3.4 86,315 - -1.0 86,315 - -1.3 86,317 + -0.1 86,325 - -1.2 86,351 - -0.5 86,352 + -0.0 86,361 + -0.9 86,361 + -0.3 86,361 - -0.7 86,369 - -2.0 86,373 + -1.4 86,401 + -0.7 86,401 + +0.1 86,401 + -0.7 86,425 - -1.4 86,425 - -0.7 86,440 - -0.6 86,447 + +0.1 86,475 + -0.7 86,493 + -0.4 86,520 - -1.7 86,530 - -1.8 86,535 - +0.5 86,536 - -1.0 86,559 + -0.8 86,567 + +0.6 86,572 + +0.3 86,575 - -1.4 86,580 - -1.8 86,580 - -2.0 86,586 + BBR_RS10560 0.11 -0.8 86,598 + BBR_RS10560 0.13 -1.1 86,598 + BBR_RS10560 0.13 -0.5 86,600 + BBR_RS10560 0.13 -0.8 86,606 - BBR_RS10560 0.14 -1.2 86,608 - BBR_RS10560 0.15 -2.3 86,612 + BBR_RS10560 0.15 +0.4 86,612 + BBR_RS10560 0.15 +0.1 86,617 + BBR_RS10560 0.16 -1.9 86,619 - BBR_RS10560 0.17 -0.7 86,655 + BBR_RS10560 0.23 -0.7 86,655 + BBR_RS10560 0.23 -0.6 86,663 - BBR_RS10560 0.24 -1.9 86,663 - BBR_RS10560 0.24 +0.0 86,663 - BBR_RS10560 0.24 -0.9 86,664 + BBR_RS10560 0.25 -1.8 86,664 - BBR_RS10560 0.25 -1.3 86,665 + BBR_RS10560 0.25 -0.8 86,665 + BBR_RS10560 0.25 -0.8 86,669 + BBR_RS10560 0.25 -0.6 86,670 + BBR_RS10560 0.26 +0.5 86,672 - BBR_RS10560 0.26 -2.9 86,673 - BBR_RS10560 0.26 -1.2 86,678 - BBR_RS10560 0.27 -2.5 86,678 - BBR_RS10560 0.27 -1.3 86,685 + BBR_RS10560 0.28 -0.5 86,687 - BBR_RS10560 0.29 -2.5 86,719 - BBR_RS10560 0.34 -1.1 86,724 - BBR_RS10560 0.35 -1.4 86,728 + BBR_RS10560 0.36 -1.7 86,728 + BBR_RS10560 0.36 -0.6 86,733 + BBR_RS10560 0.37 -0.3 86,733 + BBR_RS10560 0.37 -2.5 86,734 - BBR_RS10560 0.37 -1.7 86,741 - BBR_RS10560 0.38 +0.9 86,747 - BBR_RS10560 0.39 -1.7 86,760 + BBR_RS10560 0.41 -0.7 86,760 + BBR_RS10560 0.41 -0.2 86,785 - BBR_RS10560 0.46 -2.1 86,785 - BBR_RS10560 0.46 -1.6 86,789 + BBR_RS10560 0.46 -0.4 86,794 + BBR_RS10560 0.47 -1.4
Or see this region's nucleotide sequence