Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17465

Experiment: Trans-2-methyl-2-butenoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17460 and ABZR87_RS17465 are separated by 61 nucleotidesABZR87_RS17465 and ABZR87_RS17470 are separated by 72 nucleotides ABZR87_RS17460: ABZR87_RS17460 - acyl-CoA dehydrogenase, at 276,058 to 277,191 _RS17460 ABZR87_RS17465: ABZR87_RS17465 - AMP-binding protein, at 277,253 to 278,947 _RS17465 ABZR87_RS17470: ABZR87_RS17470 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 279,020 to 280,567 _RS17470 Position (kb) 277 278 279Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 276.254 kb on - strand, within ABZR87_RS17460at 276.263 kb on - strand, within ABZR87_RS17460at 276.263 kb on - strand, within ABZR87_RS17460at 276.386 kb on - strand, within ABZR87_RS17460at 276.386 kb on - strand, within ABZR87_RS17460at 276.848 kb on - strand, within ABZR87_RS17460at 277.021 kb on + strand, within ABZR87_RS17460at 277.022 kb on - strand, within ABZR87_RS17460at 277.022 kb on - strand, within ABZR87_RS17460at 277.084 kb on + strandat 277.096 kb on + strandat 277.141 kb on + strandat 277.141 kb on + strandat 277.142 kb on - strandat 277.142 kb on - strandat 277.142 kb on - strandat 277.189 kb on + strandat 277.190 kb on - strandat 277.190 kb on - strandat 277.190 kb on - strandat 277.267 kb on - strandat 277.267 kb on - strandat 277.389 kb on - strandat 277.389 kb on - strandat 277.717 kb on + strand, within ABZR87_RS17465at 277.718 kb on - strand, within ABZR87_RS17465at 277.718 kb on - strand, within ABZR87_RS17465at 277.800 kb on - strand, within ABZR87_RS17465at 277.862 kb on + strand, within ABZR87_RS17465at 277.863 kb on - strand, within ABZR87_RS17465at 277.947 kb on - strand, within ABZR87_RS17465at 278.064 kb on - strand, within ABZR87_RS17465at 278.070 kb on - strand, within ABZR87_RS17465at 278.070 kb on - strand, within ABZR87_RS17465at 278.116 kb on + strand, within ABZR87_RS17465at 278.183 kb on + strand, within ABZR87_RS17465at 278.184 kb on - strand, within ABZR87_RS17465at 278.513 kb on + strand, within ABZR87_RS17465at 278.513 kb on + strand, within ABZR87_RS17465at 278.516 kb on + strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.564 kb on + strand, within ABZR87_RS17465at 278.565 kb on - strand, within ABZR87_RS17465at 278.565 kb on - strand, within ABZR87_RS17465at 278.798 kb on + strandat 278.799 kb on - strandat 278.925 kb on - strandat 279.474 kb on - strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470

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Per-strain Table

Position Strand Gene LocusTag Fraction Trans-2-methyl-2-butenoic (C)
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276,254 - ABZR87_RS17460 0.17 -2.2
276,263 - ABZR87_RS17460 0.18 -3.5
276,263 - ABZR87_RS17460 0.18 -2.2
276,386 - ABZR87_RS17460 0.29 -2.7
276,386 - ABZR87_RS17460 0.29 -0.8
276,848 - ABZR87_RS17460 0.70 -1.8
277,021 + ABZR87_RS17460 0.85 -1.1
277,022 - ABZR87_RS17460 0.85 -2.3
277,022 - ABZR87_RS17460 0.85 +0.6
277,084 + -0.8
277,096 + -0.3
277,141 + -0.3
277,141 + +0.3
277,142 - -0.6
277,142 - -0.6
277,142 - -3.0
277,189 + -2.9
277,190 - -0.7
277,190 - -1.0
277,190 - -2.0
277,267 - -3.4
277,267 - -1.7
277,389 - -3.8
277,389 - +0.2
277,717 + ABZR87_RS17465 0.27 -2.6
277,718 - ABZR87_RS17465 0.27 -0.9
277,718 - ABZR87_RS17465 0.27 -3.3
277,800 - ABZR87_RS17465 0.32 -2.7
277,862 + ABZR87_RS17465 0.36 -1.2
277,863 - ABZR87_RS17465 0.36 -0.8
277,947 - ABZR87_RS17465 0.41 -0.9
278,064 - ABZR87_RS17465 0.48 -1.4
278,070 - ABZR87_RS17465 0.48 -1.2
278,070 - ABZR87_RS17465 0.48 -1.6
278,116 + ABZR87_RS17465 0.51 -0.4
278,183 + ABZR87_RS17465 0.55 +0.5
278,184 - ABZR87_RS17465 0.55 -3.4
278,513 + ABZR87_RS17465 0.74 -2.1
278,513 + ABZR87_RS17465 0.74 -1.4
278,516 + ABZR87_RS17465 0.75 +0.6
278,517 - ABZR87_RS17465 0.75 -2.9
278,517 - ABZR87_RS17465 0.75 -3.1
278,517 - ABZR87_RS17465 0.75 -1.1
278,564 + ABZR87_RS17465 0.77 -1.5
278,565 - ABZR87_RS17465 0.77 -3.2
278,565 - ABZR87_RS17465 0.77 -1.8
278,798 + -3.0
278,799 - -3.8
278,925 - -1.5
279,474 - ABZR87_RS17470 0.29 -2.0
279,773 + ABZR87_RS17470 0.49 -1.3
279,773 + ABZR87_RS17470 0.49 +0.3
279,773 + ABZR87_RS17470 0.49 -0.3
279,774 - ABZR87_RS17470 0.49 -0.5
279,774 - ABZR87_RS17470 0.49 -1.4

Or see this region's nucleotide sequence