Experiment: Trans-2-methyl-2-butenoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS06315 and ABZR87_RS06320 are separated by 158 nucleotides ABZR87_RS06320 and ABZR87_RS06325 are separated by 176 nucleotides ABZR87_RS06325 and ABZR87_RS06330 are separated by 184 nucleotides
ABZR87_RS06315: ABZR87_RS06315 - bifunctional isocitrate dehydrogenase kinase/phosphatase, at 1,348,705 to 1,350,540
_RS06315
ABZR87_RS06320: ABZR87_RS06320 - isovaleryl-CoA dehydrogenase, at 1,350,699 to 1,351,880
_RS06320
ABZR87_RS06325: ABZR87_RS06325 - MerR family DNA-binding transcriptional regulator, at 1,352,057 to 1,352,554
_RS06325
ABZR87_RS06330: ABZR87_RS06330 - MBL fold metallo-hydrolase, at 1,352,739 to 1,353,818
_RS06330
Position (kb) 1350
1351
1352 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1349.703 kb on - strand, within ABZR87_RS06315 at 1349.703 kb on - strand, within ABZR87_RS06315 at 1349.735 kb on + strand, within ABZR87_RS06315 at 1349.736 kb on - strand, within ABZR87_RS06315 at 1349.880 kb on - strand, within ABZR87_RS06315 at 1349.981 kb on + strand, within ABZR87_RS06315 at 1349.981 kb on + strand, within ABZR87_RS06315 at 1350.080 kb on + strand, within ABZR87_RS06315 at 1350.083 kb on + strand, within ABZR87_RS06315 at 1350.083 kb on + strand, within ABZR87_RS06315 at 1350.084 kb on - strand, within ABZR87_RS06315 at 1350.084 kb on - strand, within ABZR87_RS06315 at 1350.084 kb on - strand, within ABZR87_RS06315 at 1350.084 kb on - strand, within ABZR87_RS06315 at 1350.084 kb on - strand, within ABZR87_RS06315 at 1350.092 kb on + strand, within ABZR87_RS06315 at 1350.093 kb on - strand, within ABZR87_RS06315 at 1350.093 kb on - strand, within ABZR87_RS06315 at 1350.128 kb on + strand, within ABZR87_RS06315 at 1350.128 kb on + strand, within ABZR87_RS06315 at 1350.129 kb on - strand, within ABZR87_RS06315 at 1350.129 kb on - strand, within ABZR87_RS06315 at 1350.129 kb on - strand, within ABZR87_RS06315 at 1350.176 kb on + strand, within ABZR87_RS06315 at 1350.176 kb on + strand, within ABZR87_RS06315 at 1350.177 kb on - strand, within ABZR87_RS06315 at 1350.177 kb on - strand, within ABZR87_RS06315 at 1350.177 kb on - strand, within ABZR87_RS06315 at 1350.177 kb on - strand, within ABZR87_RS06315 at 1350.263 kb on + strand, within ABZR87_RS06315 at 1350.263 kb on + strand, within ABZR87_RS06315 at 1350.263 kb on + strand, within ABZR87_RS06315 at 1350.264 kb on - strand, within ABZR87_RS06315 at 1350.264 kb on - strand, within ABZR87_RS06315 at 1350.264 kb on - strand, within ABZR87_RS06315 at 1350.314 kb on + strand, within ABZR87_RS06315 at 1350.315 kb on - strand, within ABZR87_RS06315 at 1350.359 kb on + strand at 1350.362 kb on + strand at 1350.363 kb on - strand at 1350.458 kb on + strand at 1350.458 kb on + strand at 1350.459 kb on - strand at 1350.459 kb on - strand at 1350.595 kb on + strand at 1350.595 kb on + strand at 1350.595 kb on + strand at 1350.595 kb on + strand at 1350.595 kb on + strand at 1350.595 kb on + strand at 1350.596 kb on - strand at 1350.596 kb on - strand at 1350.602 kb on + strand at 1350.603 kb on - strand at 1350.603 kb on - strand at 1350.603 kb on - strand at 1350.603 kb on - strand at 1350.603 kb on - strand at 1350.603 kb on - strand at 1350.650 kb on - strand at 1350.700 kb on + strand at 1350.700 kb on + strand at 1350.700 kb on + strand at 1350.700 kb on + strand at 1350.701 kb on - strand at 1350.701 kb on - strand at 1350.701 kb on - strand at 1350.701 kb on - strand at 1350.701 kb on - strand at 1350.715 kb on + strand at 1350.715 kb on + strand at 1350.716 kb on - strand at 1350.716 kb on - strand at 1350.772 kb on + strand at 1350.820 kb on + strand, within ABZR87_RS06320 at 1350.820 kb on + strand, within ABZR87_RS06320 at 1350.821 kb on - strand, within ABZR87_RS06320 at 1350.883 kb on + strand, within ABZR87_RS06320 at 1350.884 kb on - strand, within ABZR87_RS06320 at 1350.884 kb on - strand, within ABZR87_RS06320 at 1350.964 kb on + strand, within ABZR87_RS06320 at 1350.964 kb on + strand, within ABZR87_RS06320 at 1350.964 kb on + strand, within ABZR87_RS06320 at 1350.964 kb on + strand, within ABZR87_RS06320 at 1350.965 kb on - strand, within ABZR87_RS06320 at 1350.965 kb on - strand, within ABZR87_RS06320 at 1350.965 kb on - strand, within ABZR87_RS06320 at 1350.965 kb on - strand, within ABZR87_RS06320 at 1350.965 kb on - strand, within ABZR87_RS06320 at 1350.970 kb on + strand, within ABZR87_RS06320 at 1350.970 kb on + strand, within ABZR87_RS06320 at 1350.970 kb on + strand, within ABZR87_RS06320 at 1350.970 kb on + strand, within ABZR87_RS06320 at 1350.970 kb on + strand, within ABZR87_RS06320 at 1350.971 kb on - strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.997 kb on + strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1350.998 kb on - strand, within ABZR87_RS06320 at 1351.071 kb on - strand, within ABZR87_RS06320 at 1351.071 kb on - strand, within ABZR87_RS06320 at 1351.071 kb on - strand, within ABZR87_RS06320 at 1351.071 kb on - strand, within ABZR87_RS06320 at 1351.072 kb on + strand, within ABZR87_RS06320 at 1351.073 kb on - strand, within ABZR87_RS06320 at 1351.073 kb on - strand, within ABZR87_RS06320 at 1351.073 kb on - strand, within ABZR87_RS06320 at 1351.073 kb on - strand, within ABZR87_RS06320 at 1351.357 kb on + strand, within ABZR87_RS06320 at 1351.420 kb on + strand, within ABZR87_RS06320 at 1351.420 kb on + strand, within ABZR87_RS06320 at 1351.421 kb on - strand, within ABZR87_RS06320 at 1351.421 kb on - strand, within ABZR87_RS06320 at 1351.534 kb on + strand, within ABZR87_RS06320 at 1351.534 kb on + strand, within ABZR87_RS06320 at 1351.534 kb on + strand, within ABZR87_RS06320 at 1351.534 kb on + strand, within ABZR87_RS06320 at 1351.535 kb on - strand, within ABZR87_RS06320 at 1351.535 kb on - strand, within ABZR87_RS06320 at 1351.535 kb on - strand, within ABZR87_RS06320 at 1351.535 kb on - strand, within ABZR87_RS06320 at 1351.535 kb on - strand, within ABZR87_RS06320 at 1351.603 kb on + strand, within ABZR87_RS06320 at 1351.603 kb on + strand, within ABZR87_RS06320 at 1351.603 kb on + strand, within ABZR87_RS06320 at 1351.604 kb on - strand, within ABZR87_RS06320 at 1351.604 kb on - strand, within ABZR87_RS06320 at 1351.604 kb on - strand, within ABZR87_RS06320 at 1351.666 kb on + strand, within ABZR87_RS06320 at 1351.687 kb on + strand, within ABZR87_RS06320 at 1351.687 kb on + strand, within ABZR87_RS06320 at 1351.688 kb on - strand, within ABZR87_RS06320 at 1351.922 kb on + strand at 1351.922 kb on + strand at 1351.922 kb on + strand at 1351.923 kb on - strand at 1351.923 kb on - strand at 1351.923 kb on - strand at 1351.955 kb on + strand at 1351.955 kb on + strand at 1351.955 kb on + strand at 1351.956 kb on - strand at 1351.956 kb on - strand at 1352.001 kb on - strand at 1352.001 kb on - strand at 1352.041 kb on + strand at 1352.042 kb on - strand at 1352.042 kb on - strand at 1352.045 kb on + strand at 1352.045 kb on + strand at 1352.045 kb on + strand at 1352.047 kb on - strand at 1352.259 kb on + strand, within ABZR87_RS06325 at 1352.259 kb on + strand, within ABZR87_RS06325 at 1352.259 kb on + strand, within ABZR87_RS06325 at 1352.259 kb on + strand, within ABZR87_RS06325 at 1352.260 kb on - strand, within ABZR87_RS06325 at 1352.260 kb on - strand, within ABZR87_RS06325 at 1352.260 kb on - strand, within ABZR87_RS06325 at 1352.260 kb on - strand, within ABZR87_RS06325 at 1352.260 kb on - strand, within ABZR87_RS06325 at 1352.352 kb on + strand, within ABZR87_RS06325 at 1352.352 kb on + strand, within ABZR87_RS06325 at 1352.412 kb on + strand, within ABZR87_RS06325 at 1352.412 kb on + strand, within ABZR87_RS06325 at 1352.412 kb on + strand, within ABZR87_RS06325 at 1352.413 kb on - strand, within ABZR87_RS06325 at 1352.413 kb on - strand, within ABZR87_RS06325 at 1352.413 kb on - strand, within ABZR87_RS06325 at 1352.413 kb on - strand, within ABZR87_RS06325 at 1352.468 kb on - strand, within ABZR87_RS06325 at 1352.542 kb on + strand at 1352.542 kb on + strand at 1352.543 kb on - strand at 1352.543 kb on - strand at 1352.543 kb on - strand at 1352.554 kb on + strand at 1352.554 kb on + strand at 1352.554 kb on + strand at 1352.555 kb on - strand at 1352.555 kb on - strand at 1352.555 kb on - strand at 1352.555 kb on - strand at 1352.555 kb on - strand at 1352.571 kb on - strand at 1352.571 kb on - strand at 1352.614 kb on + strand at 1352.614 kb on + strand at 1352.614 kb on + strand at 1352.615 kb on - strand at 1352.615 kb on - strand at 1352.626 kb on + strand at 1352.627 kb on - strand at 1352.658 kb on - strand at 1352.766 kb on + strand at 1352.767 kb on - strand at 1352.767 kb on - strand at 1352.767 kb on - strand at 1352.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trans-2-methyl-2-butenoic (C) remove 1,349,703 - ABZR87_RS06315 0.54 -3.0 1,349,703 - ABZR87_RS06315 0.54 -1.7 1,349,735 + ABZR87_RS06315 0.56 -2.2 1,349,736 - ABZR87_RS06315 0.56 -4.0 1,349,880 - ABZR87_RS06315 0.64 -2.2 1,349,981 + ABZR87_RS06315 0.69 -2.5 1,349,981 + ABZR87_RS06315 0.69 -4.3 1,350,080 + ABZR87_RS06315 0.75 -3.1 1,350,083 + ABZR87_RS06315 0.75 -2.9 1,350,083 + ABZR87_RS06315 0.75 -4.9 1,350,084 - ABZR87_RS06315 0.75 -3.4 1,350,084 - ABZR87_RS06315 0.75 -0.9 1,350,084 - ABZR87_RS06315 0.75 -1.7 1,350,084 - ABZR87_RS06315 0.75 -3.9 1,350,084 - ABZR87_RS06315 0.75 -2.7 1,350,092 + ABZR87_RS06315 0.76 -1.6 1,350,093 - ABZR87_RS06315 0.76 -2.0 1,350,093 - ABZR87_RS06315 0.76 -2.0 1,350,128 + ABZR87_RS06315 0.78 -1.7 1,350,128 + ABZR87_RS06315 0.78 -2.0 1,350,129 - ABZR87_RS06315 0.78 -3.0 1,350,129 - ABZR87_RS06315 0.78 -0.9 1,350,129 - ABZR87_RS06315 0.78 +0.5 1,350,176 + ABZR87_RS06315 0.80 -2.0 1,350,176 + ABZR87_RS06315 0.80 -0.4 1,350,177 - ABZR87_RS06315 0.80 -3.7 1,350,177 - ABZR87_RS06315 0.80 -1.8 1,350,177 - ABZR87_RS06315 0.80 +0.6 1,350,177 - ABZR87_RS06315 0.80 -2.9 1,350,263 + ABZR87_RS06315 0.85 -1.9 1,350,263 + ABZR87_RS06315 0.85 -2.3 1,350,263 + ABZR87_RS06315 0.85 -2.9 1,350,264 - ABZR87_RS06315 0.85 -1.0 1,350,264 - ABZR87_RS06315 0.85 +0.5 1,350,264 - ABZR87_RS06315 0.85 -2.5 1,350,314 + ABZR87_RS06315 0.88 +1.1 1,350,315 - ABZR87_RS06315 0.88 -2.2 1,350,359 + -0.9 1,350,362 + -1.8 1,350,363 - -2.7 1,350,458 + -1.6 1,350,458 + -2.9 1,350,459 - -2.5 1,350,459 - -1.3 1,350,595 + -2.6 1,350,595 + -1.2 1,350,595 + -1.5 1,350,595 + -2.2 1,350,595 + -1.2 1,350,595 + -2.0 1,350,596 - -1.0 1,350,596 - -2.7 1,350,602 + -3.3 1,350,603 - +0.6 1,350,603 - +0.2 1,350,603 - -2.5 1,350,603 - -1.1 1,350,603 - -0.6 1,350,603 - +0.2 1,350,650 - -1.5 1,350,700 + -2.3 1,350,700 + -3.2 1,350,700 + -2.0 1,350,700 + -1.5 1,350,701 - -0.6 1,350,701 - +0.7 1,350,701 - -0.2 1,350,701 - -0.6 1,350,701 - +0.2 1,350,715 + -2.4 1,350,715 + -2.8 1,350,716 - -0.6 1,350,716 - -0.7 1,350,772 + -1.3 1,350,820 + ABZR87_RS06320 0.10 -1.7 1,350,820 + ABZR87_RS06320 0.10 -1.7 1,350,821 - ABZR87_RS06320 0.10 -1.4 1,350,883 + ABZR87_RS06320 0.16 -2.9 1,350,884 - ABZR87_RS06320 0.16 -1.6 1,350,884 - ABZR87_RS06320 0.16 -2.9 1,350,964 + ABZR87_RS06320 0.22 -2.8 1,350,964 + ABZR87_RS06320 0.22 -0.1 1,350,964 + ABZR87_RS06320 0.22 -3.5 1,350,964 + ABZR87_RS06320 0.22 -3.8 1,350,965 - ABZR87_RS06320 0.23 -1.5 1,350,965 - ABZR87_RS06320 0.23 -0.2 1,350,965 - ABZR87_RS06320 0.23 -0.0 1,350,965 - ABZR87_RS06320 0.23 -2.6 1,350,965 - ABZR87_RS06320 0.23 -0.5 1,350,970 + ABZR87_RS06320 0.23 -3.0 1,350,970 + ABZR87_RS06320 0.23 -2.8 1,350,970 + ABZR87_RS06320 0.23 -2.5 1,350,970 + ABZR87_RS06320 0.23 -2.8 1,350,970 + ABZR87_RS06320 0.23 -0.6 1,350,971 - ABZR87_RS06320 0.23 +0.6 1,350,997 + ABZR87_RS06320 0.25 -3.2 1,350,997 + ABZR87_RS06320 0.25 -3.1 1,350,997 + ABZR87_RS06320 0.25 -1.9 1,350,997 + ABZR87_RS06320 0.25 -3.0 1,350,997 + ABZR87_RS06320 0.25 -2.6 1,350,997 + ABZR87_RS06320 0.25 -2.3 1,350,997 + ABZR87_RS06320 0.25 -2.2 1,350,997 + ABZR87_RS06320 0.25 -0.9 1,350,997 + ABZR87_RS06320 0.25 -2.3 1,350,997 + ABZR87_RS06320 0.25 -4.1 1,350,997 + ABZR87_RS06320 0.25 -3.8 1,350,998 - ABZR87_RS06320 0.25 -1.2 1,350,998 - ABZR87_RS06320 0.25 -2.3 1,350,998 - ABZR87_RS06320 0.25 -1.7 1,350,998 - ABZR87_RS06320 0.25 -2.1 1,350,998 - ABZR87_RS06320 0.25 -2.0 1,350,998 - ABZR87_RS06320 0.25 -1.5 1,350,998 - ABZR87_RS06320 0.25 -0.1 1,350,998 - ABZR87_RS06320 0.25 +0.1 1,351,071 - ABZR87_RS06320 0.31 -1.7 1,351,071 - ABZR87_RS06320 0.31 -1.1 1,351,071 - ABZR87_RS06320 0.31 -3.2 1,351,071 - ABZR87_RS06320 0.31 -1.5 1,351,072 + ABZR87_RS06320 0.32 -1.5 1,351,073 - ABZR87_RS06320 0.32 -1.6 1,351,073 - ABZR87_RS06320 0.32 -0.4 1,351,073 - ABZR87_RS06320 0.32 -1.0 1,351,073 - ABZR87_RS06320 0.32 -1.3 1,351,357 + ABZR87_RS06320 0.56 -3.8 1,351,420 + ABZR87_RS06320 0.61 -2.6 1,351,420 + ABZR87_RS06320 0.61 -3.1 1,351,421 - ABZR87_RS06320 0.61 -0.6 1,351,421 - ABZR87_RS06320 0.61 -2.9 1,351,534 + ABZR87_RS06320 0.71 -3.6 1,351,534 + ABZR87_RS06320 0.71 -2.5 1,351,534 + ABZR87_RS06320 0.71 -3.4 1,351,534 + ABZR87_RS06320 0.71 -2.6 1,351,535 - ABZR87_RS06320 0.71 -2.3 1,351,535 - ABZR87_RS06320 0.71 -3.3 1,351,535 - ABZR87_RS06320 0.71 -0.3 1,351,535 - ABZR87_RS06320 0.71 -2.6 1,351,535 - ABZR87_RS06320 0.71 +0.1 1,351,603 + ABZR87_RS06320 0.76 -3.0 1,351,603 + ABZR87_RS06320 0.76 -3.4 1,351,603 + ABZR87_RS06320 0.76 -3.4 1,351,604 - ABZR87_RS06320 0.77 -3.7 1,351,604 - ABZR87_RS06320 0.77 -2.2 1,351,604 - ABZR87_RS06320 0.77 -1.1 1,351,666 + ABZR87_RS06320 0.82 -1.6 1,351,687 + ABZR87_RS06320 0.84 -3.7 1,351,687 + ABZR87_RS06320 0.84 -2.1 1,351,688 - ABZR87_RS06320 0.84 -3.7 1,351,922 + -3.2 1,351,922 + -1.6 1,351,922 + -2.1 1,351,923 - -1.0 1,351,923 - -0.8 1,351,923 - -0.9 1,351,955 + -3.6 1,351,955 + -3.5 1,351,955 + -3.3 1,351,956 - -1.2 1,351,956 - -2.0 1,352,001 - -1.9 1,352,001 - -1.3 1,352,041 + -2.9 1,352,042 - +0.5 1,352,042 - -0.1 1,352,045 + -1.1 1,352,045 + -1.9 1,352,045 + -0.9 1,352,047 - -0.4 1,352,259 + ABZR87_RS06325 0.41 +0.9 1,352,259 + ABZR87_RS06325 0.41 -0.1 1,352,259 + ABZR87_RS06325 0.41 -0.5 1,352,259 + ABZR87_RS06325 0.41 -0.9 1,352,260 - ABZR87_RS06325 0.41 +0.6 1,352,260 - ABZR87_RS06325 0.41 +0.5 1,352,260 - ABZR87_RS06325 0.41 -0.2 1,352,260 - ABZR87_RS06325 0.41 +0.2 1,352,260 - ABZR87_RS06325 0.41 +0.2 1,352,352 + ABZR87_RS06325 0.59 -0.6 1,352,352 + ABZR87_RS06325 0.59 -0.4 1,352,412 + ABZR87_RS06325 0.71 +0.2 1,352,412 + ABZR87_RS06325 0.71 +0.0 1,352,412 + ABZR87_RS06325 0.71 -0.6 1,352,413 - ABZR87_RS06325 0.71 +0.1 1,352,413 - ABZR87_RS06325 0.71 +1.1 1,352,413 - ABZR87_RS06325 0.71 +0.5 1,352,413 - ABZR87_RS06325 0.71 -0.9 1,352,468 - ABZR87_RS06325 0.83 -0.8 1,352,542 + +1.3 1,352,542 + -1.8 1,352,543 - +1.7 1,352,543 - +0.0 1,352,543 - -1.5 1,352,554 + -0.3 1,352,554 + +0.1 1,352,554 + +0.8 1,352,555 - -0.8 1,352,555 - +0.4 1,352,555 - -0.1 1,352,555 - +0.2 1,352,555 - -0.0 1,352,571 - +0.1 1,352,571 - -0.3 1,352,614 + +1.2 1,352,614 + -0.1 1,352,614 + -0.5 1,352,615 - -0.4 1,352,615 - +0.0 1,352,626 + -0.3 1,352,627 - +0.2 1,352,658 - -0.5 1,352,766 + -0.3 1,352,767 - -1.3 1,352,767 - -0.9 1,352,767 - +0.5 1,352,827 - +0.3
Or see this region's nucleotide sequence