Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1677 and Shew_1678 are separated by 72 nucleotides Shew_1678 and Shew_1679 are separated by 60 nucleotides Shew_1679 and Shew_1680 are separated by 128 nucleotides Shew_1680 and Shew_1681 are separated by 218 nucleotides
Shew_1677: Shew_1677 - leucyl aminopeptidase (RefSeq), at 1,928,104 to 1,929,465
_1677
Shew_1678: Shew_1678 - hypothetical protein (RefSeq), at 1,929,538 to 1,929,966
_1678
Shew_1679: Shew_1679 - TetR family transcriptional regulator (RefSeq), at 1,930,027 to 1,930,584
_1679
Shew_1680: Shew_1680 - HPP family protein+B94 (RefSeq), at 1,930,713 to 1,931,183
_1680
Shew_1681: Shew_1681 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 1,931,402 to 1,932,892
_1681
Position (kb) 1930
1931 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1929.031 kb on - strand, within Shew_1677 at 1929.379 kb on - strand at 1929.379 kb on - strand at 1929.420 kb on + strand at 1929.656 kb on - strand, within Shew_1678 at 1930.090 kb on - strand, within Shew_1679 at 1930.370 kb on + strand, within Shew_1679 at 1930.689 kb on + strand at 1931.364 kb on + strand at 1931.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 1,929,031 - Shew_1677 0.68 +0.1 1,929,379 - +0.8 1,929,379 - -0.3 1,929,420 + +0.1 1,929,656 - Shew_1678 0.28 +0.9 1,930,090 - Shew_1679 0.11 -2.8 1,930,370 + Shew_1679 0.61 -3.1 1,930,689 + +0.4 1,931,364 + -0.6 1,931,420 - +0.8
Or see this region's nucleotide sequence