Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1311 and Shew_1312 are separated by 3 nucleotides Shew_1312 and Shew_1313 are separated by 142 nucleotides Shew_1313 and Shew_1314 are separated by 213 nucleotides
Shew_1311: Shew_1311 - acriflavin resistance protein (RefSeq), at 1,520,737 to 1,523,808
_1311
Shew_1312: Shew_1312 - RND family efflux transporter MFP subunit (RefSeq), at 1,523,812 to 1,524,927
_1312
Shew_1313: Shew_1313 - TetR family transcriptional regulator (RefSeq), at 1,525,070 to 1,525,681
_1313
Shew_1314: Shew_1314 - hypothetical protein (RefSeq), at 1,525,895 to 1,526,809
_1314
Position (kb)
1523
1524
1525 Strain fitness (log2 ratio)
-1
0
1 at 1522.833 kb on - strand, within Shew_1311 at 1523.014 kb on - strand, within Shew_1311 at 1523.045 kb on + strand, within Shew_1311 at 1523.121 kb on - strand, within Shew_1311 at 1523.871 kb on + strand at 1523.872 kb on + strand at 1523.971 kb on + strand, within Shew_1312 at 1524.097 kb on - strand, within Shew_1312 at 1524.582 kb on - strand, within Shew_1312 at 1524.870 kb on + strand at 1524.870 kb on + strand at 1525.008 kb on + strand at 1525.082 kb on + strand at 1525.084 kb on - strand at 1525.205 kb on - strand, within Shew_1313 at 1525.282 kb on + strand, within Shew_1313 at 1525.868 kb on - strand at 1525.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 1,522,833 - Shew_1311 0.68 +0.5 1,523,014 - Shew_1311 0.74 +0.2 1,523,045 + Shew_1311 0.75 +1.1 1,523,121 - Shew_1311 0.78 +0.1 1,523,871 + +0.8 1,523,872 + -0.4 1,523,971 + Shew_1312 0.14 +0.6 1,524,097 - Shew_1312 0.26 +0.3 1,524,582 - Shew_1312 0.69 +0.4 1,524,870 + +0.9 1,524,870 + +0.4 1,525,008 + -0.1 1,525,082 + +1.1 1,525,084 - +0.9 1,525,205 - Shew_1313 0.22 +0.5 1,525,282 + Shew_1313 0.35 +0.3 1,525,868 - +0.5 1,525,887 - -0.0
Or see this region's nucleotide sequence