Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_697

Experiment: Benzalkonium Chloride 0.016 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_695 and Pf6N2E2_696 overlap by 4 nucleotidesPf6N2E2_696 and Pf6N2E2_697 are separated by 40 nucleotidesPf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotidesPf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotidesPf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011 _695 Pf6N2E2_696 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 803,008 to 803,850 _696 Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069 _697 Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634 _698 Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052 _699 Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557 _700 Position (kb) 803 804 805 806Strain fitness (log2 ratio) -2 -1 0 1at 802.917 kb on + strandat 802.917 kb on + strandat 802.917 kb on + strandat 802.917 kb on + strandat 802.918 kb on - strandat 802.918 kb on - strandat 802.982 kb on + strandat 802.982 kb on + strandat 802.983 kb on - strandat 802.985 kb on + strandat 802.985 kb on + strandat 802.985 kb on + strandat 802.986 kb on - strandat 802.986 kb on - strandat 802.986 kb on - strandat 803.232 kb on + strand, within Pf6N2E2_696at 803.232 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.429 kb on + strand, within Pf6N2E2_696at 803.429 kb on + strand, within Pf6N2E2_696at 803.430 kb on - strand, within Pf6N2E2_696at 803.430 kb on - strand, within Pf6N2E2_696at 803.499 kb on - strand, within Pf6N2E2_696at 803.646 kb on - strand, within Pf6N2E2_696at 803.705 kb on + strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.191 kb on - strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.486 kb on - strand, within Pf6N2E2_697at 804.493 kb on - strand, within Pf6N2E2_697at 804.681 kb on + strand, within Pf6N2E2_697at 804.682 kb on - strand, within Pf6N2E2_697at 804.682 kb on - strand, within Pf6N2E2_697at 804.777 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.931 kb on - strand, within Pf6N2E2_697at 805.070 kb on - strandat 805.070 kb on - strandat 805.070 kb on - strandat 805.070 kb on - strandat 805.210 kb on + strand, within Pf6N2E2_698at 805.211 kb on - strand, within Pf6N2E2_698at 805.623 kb on - strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.769 kb on + strand, within Pf6N2E2_699at 805.769 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.907 kb on - strand, within Pf6N2E2_699

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.016 mg/ml
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802,917 + +0.8
802,917 + -0.6
802,917 + +0.4
802,917 + +1.3
802,918 - +0.2
802,918 - +0.2
802,982 + -1.1
802,982 + +1.1
802,983 - +0.4
802,985 + -0.9
802,985 + +0.7
802,985 + -0.0
802,986 - +0.1
802,986 - -2.6
802,986 - +0.7
803,232 + Pf6N2E2_696 0.27 +0.3
803,232 + Pf6N2E2_696 0.27 +0.2
803,297 + Pf6N2E2_696 0.34 +0.4
803,297 + Pf6N2E2_696 0.34 +0.4
803,297 + Pf6N2E2_696 0.34 -0.5
803,297 + Pf6N2E2_696 0.34 +0.5
803,429 + Pf6N2E2_696 0.50 -0.8
803,429 + Pf6N2E2_696 0.50 +0.9
803,430 - Pf6N2E2_696 0.50 +0.4
803,430 - Pf6N2E2_696 0.50 -0.4
803,499 - Pf6N2E2_696 0.58 -0.5
803,646 - Pf6N2E2_696 0.76 -0.8
803,705 + Pf6N2E2_696 0.83 +0.2
803,706 - Pf6N2E2_696 0.83 +0.5
803,706 - Pf6N2E2_696 0.83 -0.2
803,706 - Pf6N2E2_696 0.83 +0.1
804,165 + Pf6N2E2_697 0.23 -1.3
804,165 + Pf6N2E2_697 0.23 -0.3
804,165 + Pf6N2E2_697 0.23 +0.2
804,165 + Pf6N2E2_697 0.23 -0.8
804,191 - Pf6N2E2_697 0.25 -0.6
804,456 + Pf6N2E2_697 0.48 +1.1
804,456 + Pf6N2E2_697 0.48 +0.8
804,456 + Pf6N2E2_697 0.48 -0.6
804,457 - Pf6N2E2_697 0.48 -0.6
804,457 - Pf6N2E2_697 0.48 +0.7
804,457 - Pf6N2E2_697 0.48 -0.4
804,457 - Pf6N2E2_697 0.48 -1.1
804,486 - Pf6N2E2_697 0.50 -0.5
804,493 - Pf6N2E2_697 0.51 -0.9
804,681 + Pf6N2E2_697 0.67 -1.0
804,682 - Pf6N2E2_697 0.67 -0.5
804,682 - Pf6N2E2_697 0.67 +0.1
804,777 + Pf6N2E2_697 0.75 -0.8
804,930 + Pf6N2E2_697 0.88 -1.1
804,930 + Pf6N2E2_697 0.88 +0.1
804,930 + Pf6N2E2_697 0.88 -0.9
804,930 + Pf6N2E2_697 0.88 -0.6
804,930 + Pf6N2E2_697 0.88 -0.5
804,930 + Pf6N2E2_697 0.88 +0.4
804,931 - Pf6N2E2_697 0.88 +0.3
805,070 - +0.3
805,070 - +0.4
805,070 - -1.2
805,070 - -0.5
805,210 + Pf6N2E2_698 0.21 -0.5
805,211 - Pf6N2E2_698 0.21 -0.3
805,623 - +1.2
805,637 + -0.4
805,637 + +1.4
805,637 + +0.2
805,637 + -0.0
805,637 + -0.5
805,637 + -0.6
805,637 + -0.1
805,637 + -0.5
805,638 - -2.2
805,638 - +0.5
805,638 - +0.1
805,638 - +0.8
805,638 - -1.0
805,638 - -0.2
805,638 - +0.7
805,638 - +0.0
805,638 - -0.2
805,638 - -0.0
805,769 + Pf6N2E2_699 0.36 +0.6
805,769 + Pf6N2E2_699 0.36 -1.6
805,906 + Pf6N2E2_699 0.67 -0.6
805,906 + Pf6N2E2_699 0.67 +0.6
805,907 - Pf6N2E2_699 0.67 -0.6

Or see this region's nucleotide sequence