Experiment: Benzalkonium Chloride 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3647 and Pf6N2E2_3648 are separated by 103 nucleotides Pf6N2E2_3648 and Pf6N2E2_3649 are separated by 11 nucleotides Pf6N2E2_3649 and Pf6N2E2_3650 are separated by 60 nucleotides
Pf6N2E2_3647 - LysR family transcriptional regulator PA4989, at 4,248,765 to 4,249,655
_3647
Pf6N2E2_3648 - Ethidium bromide-methyl viologen resistance protein EmrE, at 4,249,759 to 4,250,091
_3648
Pf6N2E2_3649 - hypothetical protein, at 4,250,103 to 4,250,252
_3649
Pf6N2E2_3650 - Oxidoreductase, FAD-binding, at 4,250,313 to 4,251,488
_3650
Position (kb)
4249
4250
4251 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4248.985 kb on + strand, within Pf6N2E2_3647 at 4248.985 kb on + strand, within Pf6N2E2_3647 at 4248.985 kb on + strand, within Pf6N2E2_3647 at 4248.985 kb on + strand, within Pf6N2E2_3647 at 4248.986 kb on - strand, within Pf6N2E2_3647 at 4248.986 kb on - strand, within Pf6N2E2_3647 at 4248.986 kb on - strand, within Pf6N2E2_3647 at 4248.986 kb on - strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.099 kb on + strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.100 kb on - strand, within Pf6N2E2_3647 at 4249.198 kb on + strand, within Pf6N2E2_3647 at 4249.199 kb on - strand, within Pf6N2E2_3647 at 4249.199 kb on - strand, within Pf6N2E2_3647 at 4249.333 kb on + strand, within Pf6N2E2_3647 at 4249.333 kb on + strand, within Pf6N2E2_3647 at 4249.681 kb on - strand at 4249.707 kb on + strand at 4249.714 kb on - strand at 4249.714 kb on - strand at 4249.714 kb on - strand at 4249.714 kb on - strand at 4249.758 kb on + strand at 4249.759 kb on - strand at 4249.768 kb on + strand at 4249.768 kb on + strand at 4249.768 kb on + strand at 4249.769 kb on - strand at 4249.769 kb on - strand at 4249.769 kb on - strand at 4249.769 kb on - strand at 4249.769 kb on - strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.771 kb on + strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.772 kb on - strand at 4249.774 kb on + strand at 4249.775 kb on - strand at 4249.936 kb on + strand, within Pf6N2E2_3648 at 4249.936 kb on + strand, within Pf6N2E2_3648 at 4249.993 kb on + strand, within Pf6N2E2_3648 at 4249.993 kb on + strand, within Pf6N2E2_3648 at 4249.993 kb on + strand, within Pf6N2E2_3648 at 4249.993 kb on + strand, within Pf6N2E2_3648 at 4249.994 kb on - strand, within Pf6N2E2_3648 at 4249.994 kb on - strand, within Pf6N2E2_3648 at 4249.994 kb on - strand, within Pf6N2E2_3648 at 4249.994 kb on - strand, within Pf6N2E2_3648 at 4250.092 kb on + strand at 4250.092 kb on + strand at 4250.093 kb on - strand at 4250.278 kb on - strand at 4250.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.016 mg/ml remove 4,248,985 + Pf6N2E2_3647 0.25 -4.1 4,248,985 + Pf6N2E2_3647 0.25 -2.2 4,248,985 + Pf6N2E2_3647 0.25 -1.6 4,248,985 + Pf6N2E2_3647 0.25 -1.6 4,248,986 - Pf6N2E2_3647 0.25 -2.3 4,248,986 - Pf6N2E2_3647 0.25 -2.7 4,248,986 - Pf6N2E2_3647 0.25 -1.4 4,248,986 - Pf6N2E2_3647 0.25 -2.0 4,249,099 + Pf6N2E2_3647 0.37 -3.0 4,249,099 + Pf6N2E2_3647 0.37 -1.3 4,249,099 + Pf6N2E2_3647 0.37 -1.7 4,249,099 + Pf6N2E2_3647 0.37 -2.7 4,249,099 + Pf6N2E2_3647 0.37 -2.3 4,249,099 + Pf6N2E2_3647 0.37 -2.5 4,249,099 + Pf6N2E2_3647 0.37 -1.7 4,249,099 + Pf6N2E2_3647 0.37 -0.5 4,249,099 + Pf6N2E2_3647 0.37 -2.3 4,249,100 - Pf6N2E2_3647 0.38 -1.9 4,249,100 - Pf6N2E2_3647 0.38 -3.0 4,249,100 - Pf6N2E2_3647 0.38 -2.4 4,249,100 - Pf6N2E2_3647 0.38 -3.9 4,249,100 - Pf6N2E2_3647 0.38 -2.4 4,249,100 - Pf6N2E2_3647 0.38 -3.7 4,249,100 - Pf6N2E2_3647 0.38 -1.4 4,249,100 - Pf6N2E2_3647 0.38 -3.1 4,249,198 + Pf6N2E2_3647 0.49 -1.1 4,249,199 - Pf6N2E2_3647 0.49 -2.2 4,249,199 - Pf6N2E2_3647 0.49 -1.4 4,249,333 + Pf6N2E2_3647 0.64 -1.8 4,249,333 + Pf6N2E2_3647 0.64 -1.3 4,249,681 - -0.1 4,249,707 + -0.0 4,249,714 - -1.5 4,249,714 - -3.0 4,249,714 - -2.7 4,249,714 - -2.3 4,249,758 + -3.0 4,249,759 - -3.3 4,249,768 + -2.2 4,249,768 + -3.7 4,249,768 + -2.3 4,249,769 - -4.6 4,249,769 - -1.9 4,249,769 - -2.2 4,249,769 - -2.2 4,249,769 - -3.0 4,249,771 + -3.5 4,249,771 + -3.3 4,249,771 + -3.3 4,249,771 + -2.5 4,249,771 + -3.5 4,249,771 + -2.9 4,249,771 + -0.9 4,249,771 + -3.5 4,249,772 - -5.4 4,249,772 - -3.7 4,249,772 - -2.5 4,249,772 - -2.1 4,249,772 - -2.1 4,249,772 - +1.1 4,249,772 - -4.9 4,249,774 + -0.9 4,249,775 - +0.1 4,249,936 + Pf6N2E2_3648 0.53 -1.6 4,249,936 + Pf6N2E2_3648 0.53 -3.9 4,249,993 + Pf6N2E2_3648 0.70 -4.0 4,249,993 + Pf6N2E2_3648 0.70 -2.8 4,249,993 + Pf6N2E2_3648 0.70 -2.5 4,249,993 + Pf6N2E2_3648 0.70 -2.7 4,249,994 - Pf6N2E2_3648 0.71 -2.1 4,249,994 - Pf6N2E2_3648 0.71 -1.7 4,249,994 - Pf6N2E2_3648 0.71 -0.9 4,249,994 - Pf6N2E2_3648 0.71 -3.1 4,250,092 + -2.1 4,250,092 + -0.7 4,250,093 - -2.1 4,250,278 - -1.0 4,250,278 - -0.7
Or see this region's nucleotide sequence