Experiment: Benzalkonium Chloride 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_111 and Pf6N2E2_112 are separated by 5 nucleotides Pf6N2E2_112 and Pf6N2E2_113 overlap by 4 nucleotides Pf6N2E2_113 and Pf6N2E2_114 are separated by 104 nucleotides
Pf6N2E2_111 - FIG00954746: hypothetical protein, at 156,445 to 157,620
_111
Pf6N2E2_112 - hypothetical protein, at 157,626 to 158,315
_112
Pf6N2E2_113 - FIG00963512: hypothetical protein, at 158,312 to 160,225
_113
Pf6N2E2_114 - Polysaccharide biosynthesis protein, at 160,330 to 161,634
_114
Position (kb)
158
159
160
161 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.463 kb on - strand, within Pf6N2E2_111 at 157.559 kb on + strand at 157.664 kb on + strand at 157.665 kb on - strand at 157.694 kb on - strand at 157.736 kb on - strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.779 kb on - strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.807 kb on - strand, within Pf6N2E2_112 at 157.934 kb on - strand, within Pf6N2E2_112 at 157.984 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.039 kb on - strand, within Pf6N2E2_112 at 158.053 kb on + strand, within Pf6N2E2_112 at 158.171 kb on + strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.236 kb on + strand, within Pf6N2E2_112 at 158.237 kb on - strand, within Pf6N2E2_112 at 158.461 kb on - strand at 158.576 kb on + strand, within Pf6N2E2_113 at 158.576 kb on + strand, within Pf6N2E2_113 at 158.577 kb on - strand, within Pf6N2E2_113 at 158.943 kb on + strand, within Pf6N2E2_113 at 159.166 kb on + strand, within Pf6N2E2_113 at 159.166 kb on + strand, within Pf6N2E2_113 at 159.167 kb on - strand, within Pf6N2E2_113 at 159.167 kb on - strand, within Pf6N2E2_113 at 159.201 kb on + strand, within Pf6N2E2_113 at 159.202 kb on - strand, within Pf6N2E2_113 at 159.235 kb on - strand, within Pf6N2E2_113 at 159.241 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.507 kb on + strand, within Pf6N2E2_113 at 159.521 kb on - strand, within Pf6N2E2_113 at 159.735 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.955 kb on - strand, within Pf6N2E2_113 at 160.093 kb on - strand at 160.150 kb on - strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.297 kb on + strand at 160.387 kb on - strand at 160.427 kb on + strand at 160.428 kb on - strand at 160.460 kb on + strand at 160.461 kb on - strand, within Pf6N2E2_114 at 160.461 kb on - strand, within Pf6N2E2_114
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.016 mg/ml remove 157,462 + Pf6N2E2_111 0.86 +0.9 157,462 + Pf6N2E2_111 0.86 -0.9 157,462 + Pf6N2E2_111 0.86 -1.1 157,462 + Pf6N2E2_111 0.86 +0.2 157,463 - Pf6N2E2_111 0.87 -0.7 157,559 + -0.0 157,664 + +0.1 157,665 - +0.4 157,694 - -0.3 157,736 - Pf6N2E2_112 0.16 +1.5 157,776 + Pf6N2E2_112 0.22 -0.1 157,776 + Pf6N2E2_112 0.22 +1.5 157,776 + Pf6N2E2_112 0.22 +0.2 157,776 + Pf6N2E2_112 0.22 -0.8 157,776 + Pf6N2E2_112 0.22 -0.8 157,776 + Pf6N2E2_112 0.22 -0.7 157,776 + Pf6N2E2_112 0.22 +0.0 157,776 + Pf6N2E2_112 0.22 +0.2 157,777 - Pf6N2E2_112 0.22 -0.2 157,777 - Pf6N2E2_112 0.22 -0.4 157,777 - Pf6N2E2_112 0.22 +0.3 157,777 - Pf6N2E2_112 0.22 -0.1 157,777 - Pf6N2E2_112 0.22 +0.6 157,777 - Pf6N2E2_112 0.22 -0.6 157,777 - Pf6N2E2_112 0.22 +0.1 157,777 - Pf6N2E2_112 0.22 +1.1 157,778 + Pf6N2E2_112 0.22 +0.5 157,778 + Pf6N2E2_112 0.22 -0.3 157,779 - Pf6N2E2_112 0.22 +0.5 157,806 + Pf6N2E2_112 0.26 -1.0 157,806 + Pf6N2E2_112 0.26 +0.3 157,806 + Pf6N2E2_112 0.26 +0.3 157,807 - Pf6N2E2_112 0.26 +0.4 157,934 - Pf6N2E2_112 0.45 -0.9 157,984 + Pf6N2E2_112 0.52 +0.5 158,038 + Pf6N2E2_112 0.60 +0.1 158,038 + Pf6N2E2_112 0.60 +0.4 158,039 - Pf6N2E2_112 0.60 -0.2 158,053 + Pf6N2E2_112 0.62 +0.9 158,171 + Pf6N2E2_112 0.79 +0.2 158,212 - Pf6N2E2_112 0.85 +0.4 158,212 - Pf6N2E2_112 0.85 +0.5 158,236 + Pf6N2E2_112 0.88 +0.6 158,237 - Pf6N2E2_112 0.89 -1.8 158,461 - +0.2 158,576 + Pf6N2E2_113 0.14 +0.2 158,576 + Pf6N2E2_113 0.14 -1.7 158,577 - Pf6N2E2_113 0.14 +0.4 158,943 + Pf6N2E2_113 0.33 -0.3 159,166 + Pf6N2E2_113 0.45 +0.6 159,166 + Pf6N2E2_113 0.45 -0.5 159,167 - Pf6N2E2_113 0.45 -0.9 159,167 - Pf6N2E2_113 0.45 -0.9 159,201 + Pf6N2E2_113 0.46 -0.2 159,202 - Pf6N2E2_113 0.46 +0.7 159,235 - Pf6N2E2_113 0.48 -0.2 159,241 + Pf6N2E2_113 0.49 -0.4 159,441 + Pf6N2E2_113 0.59 -0.5 159,441 + Pf6N2E2_113 0.59 -0.2 159,441 + Pf6N2E2_113 0.59 +0.2 159,444 + Pf6N2E2_113 0.59 +1.1 159,444 + Pf6N2E2_113 0.59 +0.3 159,444 + Pf6N2E2_113 0.59 +1.2 159,444 + Pf6N2E2_113 0.59 -0.0 159,445 - Pf6N2E2_113 0.59 +0.7 159,445 - Pf6N2E2_113 0.59 -0.5 159,445 - Pf6N2E2_113 0.59 +1.9 159,507 + Pf6N2E2_113 0.62 +0.1 159,521 - Pf6N2E2_113 0.63 +0.1 159,735 + Pf6N2E2_113 0.74 +0.7 159,864 + Pf6N2E2_113 0.81 -0.4 159,864 + Pf6N2E2_113 0.81 -2.1 159,865 - Pf6N2E2_113 0.81 +0.4 159,865 - Pf6N2E2_113 0.81 -0.8 159,865 - Pf6N2E2_113 0.81 +0.1 159,865 - Pf6N2E2_113 0.81 +0.2 159,955 - Pf6N2E2_113 0.86 -0.1 160,093 - -0.9 160,150 - +0.8 160,173 + -0.5 160,173 + +0.4 160,173 + +0.3 160,297 + -0.5 160,387 - +0.3 160,427 + -0.5 160,428 - -0.6 160,460 + +0.5 160,461 - Pf6N2E2_114 0.10 -0.0 160,461 - Pf6N2E2_114 0.10 -0.5
Or see this region's nucleotide sequence