Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08585 and MMP_RS08590 are separated by 32 nucleotides MMP_RS08590 and MMP_RS08595 are separated by 10 nucleotides MMP_RS08595 and MMP_RS08600 overlap by 11 nucleotides MMP_RS08600 and MMP_RS08605 are separated by 27 nucleotides
MMP_RS08585: MMP_RS08585 - flagellin, at 1,610,636 to 1,611,286
_RS08585
MMP_RS08590: MMP_RS08590 - flagellin, at 1,611,319 to 1,611,951
_RS08590
MMP_RS08595: MMP_RS08595 - flagellar protein C, at 1,611,962 to 1,612,522
_RS08595
MMP_RS08600: MMP_RS08600 - flagellar protein D, at 1,612,512 to 1,613,480
_RS08600
MMP_RS08605: MMP_RS08605 - flagellar protein E, at 1,613,508 to 1,613,915
_RS08605
Position (kb)
1611
1612
1613 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1610.974 kb on - strand, within MMP_RS08585 at 1610.975 kb on + strand, within MMP_RS08585 at 1610.979 kb on + strand, within MMP_RS08585 at 1610.994 kb on + strand, within MMP_RS08585 at 1611.003 kb on - strand, within MMP_RS08585 at 1611.023 kb on - strand, within MMP_RS08585 at 1611.031 kb on - strand, within MMP_RS08585 at 1611.078 kb on - strand, within MMP_RS08585 at 1611.145 kb on + strand, within MMP_RS08585 at 1611.160 kb on + strand, within MMP_RS08585 at 1611.233 kb on + strand at 1611.279 kb on - strand at 1611.280 kb on + strand at 1611.284 kb on - strand at 1611.298 kb on + strand at 1611.424 kb on + strand, within MMP_RS08590 at 1611.500 kb on - strand, within MMP_RS08590 at 1611.541 kb on + strand, within MMP_RS08590 at 1611.699 kb on - strand, within MMP_RS08590 at 1611.816 kb on + strand, within MMP_RS08590 at 1611.818 kb on + strand, within MMP_RS08590 at 1611.822 kb on - strand, within MMP_RS08590 at 1611.920 kb on - strand at 1611.958 kb on - strand at 1611.962 kb on + strand at 1611.994 kb on + strand at 1612.250 kb on + strand, within MMP_RS08595 at 1612.426 kb on - strand, within MMP_RS08595 at 1612.854 kb on - strand, within MMP_RS08600 at 1612.855 kb on + strand, within MMP_RS08600 at 1613.230 kb on + strand, within MMP_RS08600 at 1613.362 kb on - strand, within MMP_RS08600 at 1613.438 kb on + strand at 1613.447 kb on + strand at 1613.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,610,974 - MMP_RS08585 0.52 -0.3 1,610,975 + MMP_RS08585 0.52 -0.1 1,610,979 + MMP_RS08585 0.53 +0.3 1,610,994 + MMP_RS08585 0.55 +0.3 1,611,003 - MMP_RS08585 0.56 -1.7 1,611,023 - MMP_RS08585 0.59 -0.9 1,611,031 - MMP_RS08585 0.61 +0.0 1,611,078 - MMP_RS08585 0.68 -4.2 1,611,145 + MMP_RS08585 0.78 -0.2 1,611,160 + MMP_RS08585 0.80 -1.7 1,611,233 + -0.0 1,611,279 - -2.4 1,611,280 + -2.1 1,611,284 - -1.5 1,611,298 + -0.4 1,611,424 + MMP_RS08590 0.17 -2.5 1,611,500 - MMP_RS08590 0.29 -2.5 1,611,541 + MMP_RS08590 0.35 -1.4 1,611,699 - MMP_RS08590 0.60 -2.1 1,611,816 + MMP_RS08590 0.79 -1.5 1,611,818 + MMP_RS08590 0.79 -1.0 1,611,822 - MMP_RS08590 0.79 -0.7 1,611,920 - -1.3 1,611,958 - -1.3 1,611,962 + -1.5 1,611,994 + -0.5 1,612,250 + MMP_RS08595 0.51 -2.4 1,612,426 - MMP_RS08595 0.83 -1.0 1,612,854 - MMP_RS08600 0.35 -1.8 1,612,855 + MMP_RS08600 0.35 -2.2 1,613,230 + MMP_RS08600 0.74 -2.0 1,613,362 - MMP_RS08600 0.88 -1.7 1,613,438 + -1.6 1,613,447 + -1.8 1,613,492 + -1.1
Or see this region's nucleotide sequence