Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07180 and MMP_RS07185 are separated by 156 nucleotides MMP_RS07185 and MMP_RS07190 are separated by 377 nucleotides
MMP_RS07180: MMP_RS07180 - DEAD/DEAH box helicase, at 1,373,639 to 1,375,897
_RS07180
MMP_RS07185: MMP_RS07185 - pyridoxal phosphate-dependent aminotransferase, at 1,376,054 to 1,377,358
_RS07185
MMP_RS07190: MMP_RS07190 - chromosome segregation protein SMC, at 1,377,736 to 1,381,305
_RS07190
Position (kb)
1376
1377
1378 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1375.299 kb on - strand, within MMP_RS07180 at 1375.299 kb on - strand, within MMP_RS07180 at 1375.300 kb on + strand, within MMP_RS07180 at 1375.454 kb on + strand, within MMP_RS07180 at 1375.565 kb on - strand, within MMP_RS07180 at 1375.676 kb on + strand at 1375.689 kb on + strand at 1375.802 kb on - strand at 1375.852 kb on - strand at 1375.889 kb on - strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.946 kb on - strand at 1375.962 kb on + strand at 1375.965 kb on - strand at 1375.966 kb on + strand at 1375.969 kb on - strand at 1376.007 kb on + strand at 1376.013 kb on + strand at 1376.013 kb on + strand at 1376.016 kb on - strand at 1376.221 kb on + strand, within MMP_RS07185 at 1376.222 kb on - strand, within MMP_RS07185 at 1376.233 kb on - strand, within MMP_RS07185 at 1376.234 kb on + strand, within MMP_RS07185 at 1376.234 kb on + strand, within MMP_RS07185 at 1376.234 kb on + strand, within MMP_RS07185 at 1376.284 kb on - strand, within MMP_RS07185 at 1376.331 kb on + strand, within MMP_RS07185 at 1376.336 kb on - strand, within MMP_RS07185 at 1376.337 kb on + strand, within MMP_RS07185 at 1376.346 kb on - strand, within MMP_RS07185 at 1376.347 kb on + strand, within MMP_RS07185 at 1376.347 kb on + strand, within MMP_RS07185 at 1376.375 kb on - strand, within MMP_RS07185 at 1376.404 kb on + strand, within MMP_RS07185 at 1376.404 kb on + strand, within MMP_RS07185 at 1376.428 kb on + strand, within MMP_RS07185 at 1376.451 kb on - strand, within MMP_RS07185 at 1376.460 kb on - strand, within MMP_RS07185 at 1376.477 kb on - strand, within MMP_RS07185 at 1376.553 kb on - strand, within MMP_RS07185 at 1376.670 kb on + strand, within MMP_RS07185 at 1376.682 kb on + strand, within MMP_RS07185 at 1376.685 kb on - strand, within MMP_RS07185 at 1376.685 kb on - strand, within MMP_RS07185 at 1376.686 kb on + strand, within MMP_RS07185 at 1376.686 kb on + strand, within MMP_RS07185 at 1376.706 kb on - strand, within MMP_RS07185 at 1376.707 kb on + strand, within MMP_RS07185 at 1376.722 kb on + strand, within MMP_RS07185 at 1376.724 kb on + strand, within MMP_RS07185 at 1376.724 kb on + strand, within MMP_RS07185 at 1377.393 kb on + strand at 1377.581 kb on + strand at 1377.590 kb on - strand at 1377.611 kb on + strand at 1377.624 kb on - strand at 1377.651 kb on - strand at 1377.652 kb on + strand at 1377.652 kb on + strand at 1377.654 kb on - strand at 1377.655 kb on + strand at 1377.655 kb on + strand at 1377.656 kb on - strand at 1377.733 kb on + strand at 1377.866 kb on - strand at 1377.867 kb on + strand at 1377.931 kb on + strand at 1378.017 kb on - strand at 1378.113 kb on + strand, within MMP_RS07190
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,375,299 - MMP_RS07180 0.73 +0.2 1,375,299 - MMP_RS07180 0.73 +0.4 1,375,300 + MMP_RS07180 0.74 +0.0 1,375,454 + MMP_RS07180 0.80 -0.3 1,375,565 - MMP_RS07180 0.85 +0.7 1,375,676 + -0.4 1,375,689 + +0.2 1,375,802 - +0.0 1,375,852 - +0.4 1,375,889 - -0.3 1,375,890 + -0.0 1,375,890 + -0.4 1,375,946 - +0.2 1,375,962 + +0.2 1,375,965 - +1.0 1,375,966 + +0.6 1,375,969 - +0.2 1,376,007 + +0.1 1,376,013 + +1.0 1,376,013 + -0.7 1,376,016 - +0.3 1,376,221 + MMP_RS07185 0.13 -4.6 1,376,222 - MMP_RS07185 0.13 -5.5 1,376,233 - MMP_RS07185 0.14 -5.1 1,376,234 + MMP_RS07185 0.14 -5.9 1,376,234 + MMP_RS07185 0.14 -5.8 1,376,234 + MMP_RS07185 0.14 -5.3 1,376,284 - MMP_RS07185 0.18 -6.0 1,376,331 + MMP_RS07185 0.21 -6.3 1,376,336 - MMP_RS07185 0.22 -4.1 1,376,337 + MMP_RS07185 0.22 -4.3 1,376,346 - MMP_RS07185 0.22 -6.3 1,376,347 + MMP_RS07185 0.22 -5.7 1,376,347 + MMP_RS07185 0.22 -5.8 1,376,375 - MMP_RS07185 0.25 -5.2 1,376,404 + MMP_RS07185 0.27 -6.2 1,376,404 + MMP_RS07185 0.27 -5.0 1,376,428 + MMP_RS07185 0.29 -5.0 1,376,451 - MMP_RS07185 0.30 -4.4 1,376,460 - MMP_RS07185 0.31 -6.1 1,376,477 - MMP_RS07185 0.32 -5.1 1,376,553 - MMP_RS07185 0.38 -4.8 1,376,670 + MMP_RS07185 0.47 -5.1 1,376,682 + MMP_RS07185 0.48 -5.5 1,376,685 - MMP_RS07185 0.48 -6.5 1,376,685 - MMP_RS07185 0.48 -6.3 1,376,686 + MMP_RS07185 0.48 -5.0 1,376,686 + MMP_RS07185 0.48 -5.2 1,376,706 - MMP_RS07185 0.50 -3.1 1,376,707 + MMP_RS07185 0.50 -5.4 1,376,722 + MMP_RS07185 0.51 -4.0 1,376,724 + MMP_RS07185 0.51 -5.5 1,376,724 + MMP_RS07185 0.51 -4.0 1,377,393 + -4.9 1,377,581 + +0.5 1,377,590 - +0.4 1,377,611 + +0.6 1,377,624 - +0.2 1,377,651 - +0.5 1,377,652 + +0.4 1,377,652 + +0.5 1,377,654 - -0.4 1,377,655 + +0.1 1,377,655 + +2.9 1,377,656 - -0.4 1,377,733 + -1.7 1,377,866 - -1.6 1,377,867 + +0.4 1,377,931 + -0.9 1,378,017 - -0.9 1,378,113 + MMP_RS07190 0.11 -1.6
Or see this region's nucleotide sequence