Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03435 and MMP_RS03440 are separated by 57 nucleotides MMP_RS03440 and MMP_RS03445 are separated by 75 nucleotides MMP_RS03445 and MMP_RS03450 are separated by 74 nucleotides MMP_RS03450 and MMP_RS03455 are separated by 97 nucleotides
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973
_RS03440
MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600
_RS03445
MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS <small>(from data)</small>, at 647,675 to 647,947
_RS03450
MMP_RS03455: MMP_RS03455 - radical SAM protein, at 648,045 to 648,938
_RS03455
Position (kb) 647
648 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 646.615 kb on - strand, within MMP_RS03435 at 646.660 kb on - strand at 646.720 kb on + strand at 646.870 kb on - strand, within MMP_RS03440 at 646.979 kb on - strand at 646.983 kb on - strand at 646.984 kb on + strand at 646.987 kb on - strand at 646.997 kb on + strand at 647.001 kb on - strand at 647.002 kb on + strand at 647.002 kb on + strand at 647.004 kb on + strand at 647.018 kb on - strand at 647.055 kb on - strand at 647.407 kb on - strand, within MMP_RS03445 at 647.695 kb on - strand at 647.797 kb on - strand, within MMP_RS03450 at 647.797 kb on - strand, within MMP_RS03450 at 647.852 kb on - strand, within MMP_RS03450 at 647.951 kb on - strand at 647.951 kb on - strand at 648.008 kb on - strand at 648.008 kb on - strand at 648.009 kb on + strand at 648.015 kb on - strand at 648.086 kb on - strand at 648.087 kb on + strand at 648.117 kb on - strand at 648.186 kb on + strand at 648.261 kb on - strand at 648.262 kb on + strand at 648.262 kb on + strand at 648.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 646,615 - MMP_RS03435 0.89 -2.6 646,660 - -4.9 646,720 + +0.1 646,870 - MMP_RS03440 0.47 -0.5 646,979 - -0.1 646,983 - +0.3 646,984 + -0.2 646,987 - -3.2 646,997 + -1.1 647,001 - -2.1 647,002 + -1.0 647,002 + -1.1 647,004 + -1.2 647,018 - -4.0 647,055 - -3.2 647,407 - MMP_RS03445 0.65 -4.1 647,695 - -4.1 647,797 - MMP_RS03450 0.45 -4.4 647,797 - MMP_RS03450 0.45 -5.9 647,852 - MMP_RS03450 0.65 -5.7 647,951 - -5.3 647,951 - -4.3 648,008 - -5.7 648,008 - -6.5 648,009 + -6.1 648,015 - -4.9 648,086 - +0.1 648,087 + -0.0 648,117 - -0.1 648,186 + -4.2 648,261 - -3.8 648,262 + -2.8 648,262 + -4.3 648,508 + -3.3
Or see this region's nucleotide sequence