Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03415 and MMP_RS03420 are separated by 13 nucleotides MMP_RS03420 and MMP_RS03425 are separated by 506 nucleotides MMP_RS03425 and MMP_RS03430 are separated by 21 nucleotides
MMP_RS03415: MMP_RS03415 - acetolactate synthase large subunit, at 641,377 to 643,140
_RS03415
MMP_RS03420: MMP_RS03420 - acetolactate synthase small subunit, at 643,154 to 643,663
_RS03420
MMP_RS03425: MMP_RS03425 - hypothetical protein, at 644,170 to 644,622
_RS03425
MMP_RS03430: MMP_RS03430 - bile acid:sodium symporter family protein, at 644,644 to 645,609
_RS03430
Position (kb) 643
644 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 642.510 kb on - strand at 642.942 kb on - strand at 643.031 kb on - strand at 643.031 kb on - strand at 643.031 kb on - strand at 643.056 kb on - strand at 643.056 kb on - strand at 643.057 kb on + strand at 643.062 kb on + strand at 643.062 kb on + strand at 643.122 kb on + strand at 643.132 kb on + strand at 643.302 kb on - strand, within MMP_RS03420 at 643.327 kb on + strand, within MMP_RS03420 at 643.576 kb on + strand, within MMP_RS03420 at 643.615 kb on - strand at 643.616 kb on + strand at 643.655 kb on + strand at 643.765 kb on + strand at 643.903 kb on + strand at 643.972 kb on - strand at 643.987 kb on - strand at 643.988 kb on + strand at 644.071 kb on + strand at 644.090 kb on + strand at 644.090 kb on + strand at 644.129 kb on - strand at 644.145 kb on + strand at 644.147 kb on + strand at 644.262 kb on - strand, within MMP_RS03425 at 644.268 kb on - strand, within MMP_RS03425 at 644.307 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.354 kb on + strand, within MMP_RS03425 at 644.419 kb on - strand, within MMP_RS03425 at 644.431 kb on + strand, within MMP_RS03425 at 644.485 kb on + strand, within MMP_RS03425 at 644.495 kb on + strand, within MMP_RS03425 at 644.562 kb on - strand, within MMP_RS03425 at 644.569 kb on - strand, within MMP_RS03425 at 644.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 642,510 - -4.0 642,942 - -5.3 643,031 - -3.4 643,031 - -5.5 643,031 - -4.5 643,056 - -6.1 643,056 - -2.9 643,057 + -4.8 643,062 + -5.1 643,062 + -4.6 643,122 + -4.8 643,132 + -3.9 643,302 - MMP_RS03420 0.29 -1.5 643,327 + MMP_RS03420 0.34 -2.5 643,576 + MMP_RS03420 0.83 -3.3 643,615 - -3.8 643,616 + -2.5 643,655 + +0.2 643,765 + +0.1 643,903 + +0.2 643,972 - -0.2 643,987 - +1.2 643,988 + -0.1 644,071 + +0.3 644,090 + -0.0 644,090 + +0.1 644,129 - -0.5 644,145 + +0.2 644,147 + +0.4 644,262 - MMP_RS03425 0.20 +0.2 644,268 - MMP_RS03425 0.22 +0.3 644,307 - MMP_RS03425 0.30 -0.0 644,324 - MMP_RS03425 0.34 +0.0 644,324 - MMP_RS03425 0.34 +0.2 644,354 + MMP_RS03425 0.41 -0.1 644,419 - MMP_RS03425 0.55 -0.2 644,431 + MMP_RS03425 0.58 +0.1 644,485 + MMP_RS03425 0.70 -0.1 644,495 + MMP_RS03425 0.72 -0.0 644,562 - MMP_RS03425 0.87 -0.3 644,569 - MMP_RS03425 0.88 +0.1 644,621 + -0.3
Or see this region's nucleotide sequence