Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS15545

Experiment: CDM1 without Uracil

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS15540 and BBR_RS15545 are separated by 63 nucleotidesBBR_RS15545 and BBR_RS15550 are separated by 91 nucleotides BBR_RS15540: BBR_RS15540 - triose-phosphate isomerase, at 1,260,738 to 1,261,541 _RS15540 BBR_RS15545: BBR_RS15545 - preprotein translocase subunit SecG, at 1,261,605 to 1,261,853 _RS15545 BBR_RS15550: BBR_RS15550 - L-lactate dehydrogenase, at 1,261,945 to 1,262,895 _RS15550 Position (kb) 1261 1262Strain fitness (log2 ratio) -3 -2 -1 0 1at 1260.635 kb on + strandat 1260.700 kb on - strandat 1261.940 kb on + strandat 1261.948 kb on - strandat 1261.987 kb on + strandat 1262.008 kb on + strandat 1262.024 kb on + strandat 1262.149 kb on - strand, within BBR_RS15550at 1262.260 kb on + strand, within BBR_RS15550at 1262.369 kb on - strand, within BBR_RS15550at 1262.409 kb on + strand, within BBR_RS15550at 1262.578 kb on - strand, within BBR_RS15550at 1262.680 kb on + strand, within BBR_RS15550

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Per-strain Table

Position Strand Gene LocusTag Fraction CDM1 without Uracil
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1,260,635 + -0.1
1,260,700 - +1.6
1,261,940 + +0.4
1,261,948 - +0.5
1,261,987 + +1.4
1,262,008 + +0.0
1,262,024 + -1.8
1,262,149 - BBR_RS15550 0.21 -2.9
1,262,260 + BBR_RS15550 0.33 -0.8
1,262,369 - BBR_RS15550 0.45 -0.8
1,262,409 + BBR_RS15550 0.49 -0.8
1,262,578 - BBR_RS15550 0.67 -2.4
1,262,680 + BBR_RS15550 0.77 -0.8

Or see this region's nucleotide sequence