Experiment: Thallium(I) acetate 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Position (kb) 416
417 Strain fitness (log2 ratio) -1
0
1 at 415.927 kb on + strand, within Shewana3_0375 at 416.057 kb on + strand at 416.148 kb on - strand at 417.114 kb on + strand at 417.461 kb on + strand, within Shewana3_0376 at 417.463 kb on + strand, within Shewana3_0376 at 417.505 kb on - strand, within Shewana3_0376 at 417.597 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.830 kb on - strand, within Shewana3_0376
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.064 mg/ml remove 415,927 + Shewana3_0375 0.88 +0.1 416,057 + -0.3 416,148 - +0.9 417,114 + -0.6 417,461 + Shewana3_0376 0.20 -0.4 417,463 + Shewana3_0376 0.21 -0.5 417,505 - Shewana3_0376 0.23 +0.9 417,597 + Shewana3_0376 0.28 -1.3 417,618 + Shewana3_0376 0.29 +0.1 417,618 + Shewana3_0376 0.29 -0.3 417,618 + Shewana3_0376 0.29 -1.7 417,830 - Shewana3_0376 0.40 +0.3
Or see this region's nucleotide sequence