Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2722 and Shew_2723 are separated by 46 nucleotides Shew_2723 and Shew_2724 are separated by 94 nucleotides Shew_2724 and Shew_2725 overlap by 7 nucleotides
Shew_2722: Shew_2722 - hypothetical protein (RefSeq), at 3,222,236 to 3,222,943
_2722
Shew_2723: Shew_2723 - NUDIX hydrolase (RefSeq), at 3,222,990 to 3,223,526
_2723
Shew_2724: Shew_2724 - indigoidine synthase A family protein (RefSeq), at 3,223,621 to 3,224,535
_2724
Shew_2725: Shew_2725 - ribokinase-like domain-containing protein (RefSeq), at 3,224,529 to 3,225,620
_2725
Position (kb)
3223
3224
3225 Strain fitness (log2 ratio)
-1
0
1 at 3222.625 kb on + strand, within Shew_2722 at 3222.633 kb on - strand, within Shew_2722 at 3223.193 kb on + strand, within Shew_2723 at 3223.247 kb on + strand, within Shew_2723 at 3223.911 kb on + strand, within Shew_2724 at 3223.911 kb on + strand, within Shew_2724 at 3223.919 kb on - strand, within Shew_2724 at 3224.291 kb on + strand, within Shew_2724 at 3224.299 kb on - strand, within Shew_2724 at 3224.402 kb on - strand, within Shew_2724 at 3224.458 kb on + strand at 3224.875 kb on + strand, within Shew_2725 at 3225.205 kb on - strand, within Shew_2725 at 3225.347 kb on + strand, within Shew_2725 at 3225.469 kb on - strand, within Shew_2725
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,222,625 + Shew_2722 0.55 +0.2 3,222,633 - Shew_2722 0.56 +0.3 3,223,193 + Shew_2723 0.38 +0.4 3,223,247 + Shew_2723 0.48 +0.7 3,223,911 + Shew_2724 0.32 +1.4 3,223,911 + Shew_2724 0.32 +1.4 3,223,919 - Shew_2724 0.33 -0.2 3,224,291 + Shew_2724 0.73 +1.1 3,224,299 - Shew_2724 0.74 +0.4 3,224,402 - Shew_2724 0.85 +0.8 3,224,458 + +0.4 3,224,875 + Shew_2725 0.32 -1.7 3,225,205 - Shew_2725 0.62 +0.3 3,225,347 + Shew_2725 0.75 +0.5 3,225,469 - Shew_2725 0.86 +0.0
Or see this region's nucleotide sequence