Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2652 and Shew_2653 are separated by 44 nucleotides Shew_2653 and queF are separated by 27 nucleotides queF and Shew_2655 are separated by 322 nucleotides
Shew_2652: Shew_2652 - hypothetical protein (RefSeq), at 3,125,582 to 3,126,355
_2652
Shew_2653: Shew_2653 - SecY interacting protein Syd (RefSeq), at 3,126,400 to 3,127,062
_2653
Shew_2654: queF - 7-cyano-7-deazaguanine reductase (RefSeq), at 3,127,090 to 3,127,980
queF
Shew_2655: Shew_2655 - hypothetical protein (RefSeq), at 3,128,303 to 3,129,493
_2655
Position (kb)
3127
3128 Strain fitness (log2 ratio)
-1
0
1 at 3126.246 kb on - strand, within Shew_2652 at 3126.875 kb on - strand, within Shew_2653 at 3127.427 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.684 kb on - strand, within queF at 3128.051 kb on + strand at 3128.059 kb on - strand at 3128.059 kb on - strand at 3128.069 kb on - strand at 3128.071 kb on - strand at 3128.442 kb on + strand, within Shew_2655 at 3128.500 kb on - strand, within Shew_2655 at 3128.636 kb on + strand, within Shew_2655 at 3128.643 kb on - strand, within Shew_2655 at 3128.752 kb on - strand, within Shew_2655 at 3128.754 kb on - strand, within Shew_2655 at 3128.960 kb on - strand, within Shew_2655
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,126,246 - Shew_2652 0.86 -0.1 3,126,875 - Shew_2653 0.72 +0.1 3,127,427 + queF Shew_2654 0.38 +1.6 3,127,676 + queF Shew_2654 0.66 +0.4 3,127,676 + queF Shew_2654 0.66 +0.6 3,127,684 - queF Shew_2654 0.67 +1.2 3,128,051 + +0.2 3,128,059 - -0.2 3,128,059 - +0.9 3,128,069 - +0.1 3,128,071 - -0.2 3,128,442 + Shew_2655 0.12 -0.2 3,128,500 - Shew_2655 0.17 +0.2 3,128,636 + Shew_2655 0.28 -0.7 3,128,643 - Shew_2655 0.29 +0.3 3,128,752 - Shew_2655 0.38 -0.1 3,128,754 - Shew_2655 0.38 -0.3 3,128,960 - Shew_2655 0.55 -0.2
Or see this region's nucleotide sequence