Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_971

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_970 and Pf6N2E2_971 are separated by 293 nucleotidesPf6N2E2_971 and Pf6N2E2_972 are separated by 43 nucleotides Pf6N2E2_970 - Transcriptional regulator, XRE family, at 1,139,218 to 1,139,475 _970 Pf6N2E2_971 - hypothetical protein, at 1,139,769 to 1,140,026 _971 Pf6N2E2_972 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 1,140,070 to 1,142,037 _972 Position (kb) 1139 1140 1141Strain fitness (log2 ratio) -1 0 1 2 3at 1138.786 kb on - strandat 1138.828 kb on - strandat 1138.828 kb on - strandat 1138.871 kb on + strandat 1138.871 kb on + strandat 1138.875 kb on + strandat 1139.220 kb on - strandat 1139.220 kb on - strandat 1139.493 kb on + strandat 1139.550 kb on + strandat 1139.550 kb on + strandat 1139.550 kb on + strandat 1139.651 kb on + strandat 1139.653 kb on + strandat 1139.743 kb on - strandat 1139.935 kb on + strand, within Pf6N2E2_971at 1139.935 kb on + strand, within Pf6N2E2_971at 1139.935 kb on + strand, within Pf6N2E2_971at 1140.033 kb on + strandat 1140.286 kb on - strand, within Pf6N2E2_972at 1140.681 kb on + strand, within Pf6N2E2_972at 1140.682 kb on - strand, within Pf6N2E2_972at 1140.851 kb on + strand, within Pf6N2E2_972at 1140.852 kb on - strand, within Pf6N2E2_972

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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1,138,786 - -0.0
1,138,828 - +0.2
1,138,828 - -0.1
1,138,871 + +0.4
1,138,871 + +1.0
1,138,875 + +0.1
1,139,220 - -0.4
1,139,220 - -0.3
1,139,493 + -0.6
1,139,550 + -0.9
1,139,550 + +0.0
1,139,550 + -0.0
1,139,651 + -0.4
1,139,653 + +1.1
1,139,743 - +1.7
1,139,935 + Pf6N2E2_971 0.64 -1.1
1,139,935 + Pf6N2E2_971 0.64 -0.4
1,139,935 + Pf6N2E2_971 0.64 -0.2
1,140,033 + +3.1
1,140,286 - Pf6N2E2_972 0.11 +0.1
1,140,681 + Pf6N2E2_972 0.31 +0.3
1,140,682 - Pf6N2E2_972 0.31 +0.4
1,140,851 + Pf6N2E2_972 0.40 -0.8
1,140,852 - Pf6N2E2_972 0.40 -1.1

Or see this region's nucleotide sequence