Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_829 and Pf6N2E2_830 are separated by 51 nucleotides Pf6N2E2_830 and Pf6N2E2_831 are separated by 57 nucleotides Pf6N2E2_831 and Pf6N2E2_832 are separated by 48 nucleotides
Pf6N2E2_829 - TesB-like acyl-CoA thioesterase 1, at 967,218 to 967,997
_829
Pf6N2E2_830 - Long-chain fatty acid transport protein, at 968,049 to 969,320
_830
Pf6N2E2_831 - Ketoacyl reductase hetN (EC 1.3.1.-), at 969,378 to 970,163
_831
Pf6N2E2_832 - Outer membrane component of tripartite multidrug resistance system, at 970,212 to 971,675
_832
Position (kb) 968
969
970 Strain fitness (log2 ratio) -2
-1
0
1
2 at 967.077 kb on - strand at 967.142 kb on + strand at 967.215 kb on + strand at 967.216 kb on - strand at 967.216 kb on - strand at 967.216 kb on - strand at 967.399 kb on + strand, within Pf6N2E2_829 at 967.399 kb on + strand, within Pf6N2E2_829 at 967.399 kb on + strand, within Pf6N2E2_829 at 967.400 kb on - strand, within Pf6N2E2_829 at 967.400 kb on - strand, within Pf6N2E2_829 at 967.928 kb on + strand at 967.991 kb on + strand at 967.992 kb on - strand at 967.992 kb on - strand at 967.995 kb on + strand at 967.996 kb on - strand at 967.998 kb on + strand at 967.998 kb on + strand at 967.999 kb on - strand at 968.036 kb on + strand at 968.036 kb on + strand at 968.036 kb on + strand at 968.037 kb on - strand at 968.037 kb on - strand at 968.037 kb on - strand at 968.037 kb on - strand at 968.037 kb on - strand at 968.044 kb on + strand at 968.044 kb on + strand at 968.045 kb on - strand at 968.045 kb on - strand at 968.045 kb on - strand at 968.047 kb on + strand at 968.047 kb on + strand at 968.047 kb on + strand at 968.048 kb on - strand at 968.144 kb on + strand at 968.144 kb on + strand at 968.145 kb on - strand at 968.145 kb on - strand at 968.145 kb on - strand at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.367 kb on + strand, within Pf6N2E2_830 at 968.368 kb on - strand, within Pf6N2E2_830 at 968.412 kb on + strand, within Pf6N2E2_830 at 968.413 kb on - strand, within Pf6N2E2_830 at 968.466 kb on + strand, within Pf6N2E2_830 at 968.517 kb on + strand, within Pf6N2E2_830 at 968.517 kb on + strand, within Pf6N2E2_830 at 968.518 kb on - strand, within Pf6N2E2_830 at 968.518 kb on - strand, within Pf6N2E2_830 at 968.518 kb on - strand, within Pf6N2E2_830 at 968.718 kb on + strand, within Pf6N2E2_830 at 968.718 kb on + strand, within Pf6N2E2_830 at 968.718 kb on + strand, within Pf6N2E2_830 at 968.736 kb on + strand, within Pf6N2E2_830 at 968.737 kb on - strand, within Pf6N2E2_830 at 968.737 kb on - strand, within Pf6N2E2_830 at 968.805 kb on + strand, within Pf6N2E2_830 at 968.806 kb on - strand, within Pf6N2E2_830 at 968.806 kb on - strand, within Pf6N2E2_830 at 968.886 kb on + strand, within Pf6N2E2_830 at 968.886 kb on + strand, within Pf6N2E2_830 at 968.887 kb on - strand, within Pf6N2E2_830 at 968.887 kb on - strand, within Pf6N2E2_830 at 968.898 kb on - strand, within Pf6N2E2_830 at 969.037 kb on - strand, within Pf6N2E2_830 at 969.037 kb on - strand, within Pf6N2E2_830 at 969.038 kb on + strand, within Pf6N2E2_830 at 969.198 kb on + strand at 969.204 kb on + strand at 969.204 kb on + strand at 969.204 kb on + strand at 969.204 kb on + strand at 969.205 kb on - strand at 969.205 kb on - strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.259 kb on - strand at 969.259 kb on - strand at 969.296 kb on + strand at 969.297 kb on - strand at 969.680 kb on - strand, within Pf6N2E2_831 at 969.697 kb on + strand, within Pf6N2E2_831 at 969.697 kb on + strand, within Pf6N2E2_831 at 969.698 kb on - strand, within Pf6N2E2_831 at 969.725 kb on - strand, within Pf6N2E2_831 at 970.087 kb on + strand at 970.195 kb on + strand at 970.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 967,077 - +0.2 967,142 + +0.5 967,215 + -0.2 967,216 - +0.5 967,216 - -0.0 967,216 - +0.7 967,399 + Pf6N2E2_829 0.23 -0.2 967,399 + Pf6N2E2_829 0.23 +0.2 967,399 + Pf6N2E2_829 0.23 +0.7 967,400 - Pf6N2E2_829 0.23 +0.3 967,400 - Pf6N2E2_829 0.23 -1.7 967,928 + +0.6 967,991 + -0.5 967,992 - +1.3 967,992 - +0.5 967,995 + -0.6 967,996 - +0.7 967,998 + -1.6 967,998 + +0.3 967,999 - -0.3 968,036 + -0.8 968,036 + -0.5 968,036 + +0.1 968,037 - -0.3 968,037 - +1.2 968,037 - -0.7 968,037 - +0.0 968,037 - -0.4 968,044 + +0.6 968,044 + -0.3 968,045 - +0.8 968,045 - +0.7 968,045 - +0.0 968,047 + +0.7 968,047 + -0.3 968,047 + -0.1 968,048 - -0.1 968,144 + +0.8 968,144 + -0.4 968,145 - -0.1 968,145 - +1.3 968,145 - +0.6 968,367 + Pf6N2E2_830 0.25 -0.6 968,367 + Pf6N2E2_830 0.25 -1.9 968,367 + Pf6N2E2_830 0.25 +0.6 968,367 + Pf6N2E2_830 0.25 -0.6 968,367 + Pf6N2E2_830 0.25 +0.2 968,367 + Pf6N2E2_830 0.25 -0.7 968,367 + Pf6N2E2_830 0.25 +0.7 968,367 + Pf6N2E2_830 0.25 +0.0 968,368 - Pf6N2E2_830 0.25 -0.9 968,412 + Pf6N2E2_830 0.29 -0.1 968,413 - Pf6N2E2_830 0.29 +1.7 968,466 + Pf6N2E2_830 0.33 +0.2 968,517 + Pf6N2E2_830 0.37 +0.3 968,517 + Pf6N2E2_830 0.37 -1.4 968,518 - Pf6N2E2_830 0.37 -0.3 968,518 - Pf6N2E2_830 0.37 +0.7 968,518 - Pf6N2E2_830 0.37 -0.7 968,718 + Pf6N2E2_830 0.53 +0.7 968,718 + Pf6N2E2_830 0.53 -0.3 968,718 + Pf6N2E2_830 0.53 -0.2 968,736 + Pf6N2E2_830 0.54 +0.3 968,737 - Pf6N2E2_830 0.54 -0.4 968,737 - Pf6N2E2_830 0.54 -0.5 968,805 + Pf6N2E2_830 0.59 -0.7 968,806 - Pf6N2E2_830 0.60 -1.4 968,806 - Pf6N2E2_830 0.60 -0.5 968,886 + Pf6N2E2_830 0.66 -0.9 968,886 + Pf6N2E2_830 0.66 +0.0 968,887 - Pf6N2E2_830 0.66 -0.3 968,887 - Pf6N2E2_830 0.66 +1.1 968,898 - Pf6N2E2_830 0.67 -0.9 969,037 - Pf6N2E2_830 0.78 +0.3 969,037 - Pf6N2E2_830 0.78 -0.3 969,038 + Pf6N2E2_830 0.78 -0.3 969,198 + +1.1 969,204 + +0.6 969,204 + +1.0 969,204 + +0.1 969,204 + -0.0 969,205 - -1.1 969,205 - -1.1 969,258 + +0.1 969,258 + -0.6 969,258 + -0.3 969,258 + +0.3 969,258 + +0.3 969,259 - +0.6 969,259 - +0.1 969,296 + -0.7 969,297 - -0.3 969,680 - Pf6N2E2_831 0.38 -0.5 969,697 + Pf6N2E2_831 0.41 -1.4 969,697 + Pf6N2E2_831 0.41 -0.1 969,698 - Pf6N2E2_831 0.41 -0.3 969,725 - Pf6N2E2_831 0.44 +2.0 970,087 + +0.3 970,195 + +0.5 970,196 - -0.5
Or see this region's nucleotide sequence